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Muscle blood flow in cats: comparison of microdialysis ethanol technique with direct measurement

Hickner, R C; Ekelund, Ulf LU ; Mellander, Stefan LU ; Ungerstedt, U and Henriksson, J (1995) In Journal of Applied Physiology 79(2). p.638-647
Abstract
A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0... (More)
A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0 microliter/min were employed. However, a linear decrease was found between 4 and approximately 45 ml.100 g-1.min-1 (r = -0.92 to -0.99). The lower outflow-to-inflow ratio was at 4 ml.100 g-1.min-1 (i.e., due to a low probe perfusion flow rate or a large dialysis membrane), the greater the sensitivity of the method was. It is concluded that this nonradioactive technique provides a simple and valid method for determining nutritive blood flow in skeletal muscle. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Applied Physiology
volume
79
issue
2
pages
638 - 647
publisher
American Physiological Society
external identifiers
  • pmid:7592229
  • scopus:0029147411
ISSN
1522-1601
language
English
LU publication?
yes
id
dd8f4a30-2af1-42bf-909e-3050ebf0b908 (old id 1109080)
alternative location
http://jap.physiology.org/cgi/reprint/79/2/638
date added to LUP
2008-07-25 13:17:48
date last changed
2017-01-01 04:49:55
@article{dd8f4a30-2af1-42bf-909e-3050ebf0b908,
  abstract     = {A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0 microliter/min were employed. However, a linear decrease was found between 4 and approximately 45 ml.100 g-1.min-1 (r = -0.92 to -0.99). The lower outflow-to-inflow ratio was at 4 ml.100 g-1.min-1 (i.e., due to a low probe perfusion flow rate or a large dialysis membrane), the greater the sensitivity of the method was. It is concluded that this nonradioactive technique provides a simple and valid method for determining nutritive blood flow in skeletal muscle.},
  author       = {Hickner, R C and Ekelund, Ulf and Mellander, Stefan and Ungerstedt, U and Henriksson, J},
  issn         = {1522-1601},
  language     = {eng},
  number       = {2},
  pages        = {638--647},
  publisher    = {American Physiological Society},
  series       = {Journal of Applied Physiology},
  title        = {Muscle blood flow in cats: comparison of microdialysis ethanol technique with direct measurement},
  volume       = {79},
  year         = {1995},
}