Muscle blood flow in cats: comparison of microdialysis ethanol technique with direct measurement
Hickner, R C ; Ekelund, Ulf LU
; Mellander, Stefan
LU
; Ungerstedt, U
and Henriksson, J
(1995)
In Journal of Applied Physiology
79(2).
p.638-647
- Abstract
- A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0... (More)
- A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0 microliter/min were employed. However, a linear decrease was found between 4 and approximately 45 ml.100 g-1.min-1 (r = -0.92 to -0.99). The lower outflow-to-inflow ratio was at 4 ml.100 g-1.min-1 (i.e., due to a low probe perfusion flow rate or a large dialysis membrane), the greater the sensitivity of the method was. It is concluded that this nonradioactive technique provides a simple and valid method for determining nutritive blood flow in skeletal muscle. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1109080
- author
- Hickner, R C
; Ekelund, Ulf
LU
; Mellander, Stefan
LU
; Ungerstedt, U
and Henriksson, J
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Applied Physiology
- volume
- 79
- issue
- 2
- pages
- 638 - 647
- publisher
- American Physiological Society
- external identifiers
-
- pmid:7592229
- scopus:0029147411
- ISSN
- 1522-1601
- language
- English
- LU publication?
- yes
- id
- dd8f4a30-2af1-42bf-909e-3050ebf0b908 (old id 1109080)
- alternative location
- http://jap.physiology.org/cgi/reprint/79/2/638
- date added to LUP
- 2016-04-01 12:05:46
- date last changed
- 2021-01-03 05:07:05
@article{dd8f4a30-2af1-42bf-909e-3050ebf0b908,
abstract = {{A quantitative validation of the microdialysis ethanol technique was performed in cat gastrocnemius muscle. Six to eight microdialysis probes were inserted into the isolated muscle preparation and perfused (0.5-10.0 microliters/min) with Krebs-Henseleit buffer containing between 5 and 1,000 mmol/l ethanol. Skeletal muscle blood flow was held constant in the range of 4-99 ml.100 g-1.min-1 by a servo-controlled roller pump and was determined with the microdialysis ethanol technique as well as by timed collection of venous outflow. The ethanol concentration outflow-to-inflow ratio ([ethanol]collected dialysate/[ethanol]infused perfusion medium) decreased in a nonlinear fashion when microdialysis perfusion flow rates of 0.5 and 1.0 microliter/min were employed. However, a linear decrease was found between 4 and approximately 45 ml.100 g-1.min-1 (r = -0.92 to -0.99). The lower outflow-to-inflow ratio was at 4 ml.100 g-1.min-1 (i.e., due to a low probe perfusion flow rate or a large dialysis membrane), the greater the sensitivity of the method was. It is concluded that this nonradioactive technique provides a simple and valid method for determining nutritive blood flow in skeletal muscle.}},
author = {{Hickner, R C and Ekelund, Ulf and Mellander, Stefan and Ungerstedt, U and Henriksson, J}},
issn = {{1522-1601}},
language = {{eng}},
number = {{2}},
pages = {{638--647}},
publisher = {{American Physiological Society}},
series = {{Journal of Applied Physiology}},
title = {{Muscle blood flow in cats: comparison of microdialysis ethanol technique with direct measurement}},
url = {{http://jap.physiology.org/cgi/reprint/79/2/638}},
volume = {{79}},
year = {{1995}},
}