Advanced

Effects of in vitro addition of captopril on copper-induced low density lipoprotein oxidation

Zieden, B; Wuttge, Dirk LU ; Karlberg, B E and Olsson, A G (1995) In British Journal of Clinical Pharmacology 39(2). p.201-203
Abstract
Low density lipoprotein (LDL) was incubated with 20 microM of the angiotensin converting enzyme (ACE) inhibitors captopril, fosinopril and quinapril or ethanol. Oxidation of LDL was initiated by addition of CuSO4 and monitored for production of conjugated dienes, thiobarbituric acid reactive substances (TBARS) and lipid peroxides. The inhibition of production of conjugated dienes was expressed as the lag phase in minutes. The lag phase for control samples was 55.2 +/- 6.1 (mean +/- s.e. mean) min and for captopril 86.4 +/- 7.0 min (P = 0.0058). Quinapril had a small but nonsignificant effect, fosinopril and ethanol had no effect. LDL incubated with captopril showed only 13.8% of TBARS and 22.7% of lipid peroxides produced by control (100%)... (More)
Low density lipoprotein (LDL) was incubated with 20 microM of the angiotensin converting enzyme (ACE) inhibitors captopril, fosinopril and quinapril or ethanol. Oxidation of LDL was initiated by addition of CuSO4 and monitored for production of conjugated dienes, thiobarbituric acid reactive substances (TBARS) and lipid peroxides. The inhibition of production of conjugated dienes was expressed as the lag phase in minutes. The lag phase for control samples was 55.2 +/- 6.1 (mean +/- s.e. mean) min and for captopril 86.4 +/- 7.0 min (P = 0.0058). Quinapril had a small but nonsignificant effect, fosinopril and ethanol had no effect. LDL incubated with captopril showed only 13.8% of TBARS and 22.7% of lipid peroxides produced by control (100%) after 1 h. Increasing concentrations of captopril showed a linear increase in the lag phase. We conclude that captopril increases the resistance of LDL to copper-induced oxidation. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
British Journal of Clinical Pharmacology
volume
39
issue
2
pages
201 - 203
publisher
John Wiley & Sons
external identifiers
  • pmid:7742164
  • scopus:0028948530
ISSN
1365-2125
language
English
LU publication?
no
id
606401cc-6c1f-4ad2-bb97-9d9ea43a3883 (old id 1109333)
alternative location
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1364963
date added to LUP
2008-07-28 10:02:07
date last changed
2017-01-01 04:40:56
@article{606401cc-6c1f-4ad2-bb97-9d9ea43a3883,
  abstract     = {Low density lipoprotein (LDL) was incubated with 20 microM of the angiotensin converting enzyme (ACE) inhibitors captopril, fosinopril and quinapril or ethanol. Oxidation of LDL was initiated by addition of CuSO4 and monitored for production of conjugated dienes, thiobarbituric acid reactive substances (TBARS) and lipid peroxides. The inhibition of production of conjugated dienes was expressed as the lag phase in minutes. The lag phase for control samples was 55.2 +/- 6.1 (mean +/- s.e. mean) min and for captopril 86.4 +/- 7.0 min (P = 0.0058). Quinapril had a small but nonsignificant effect, fosinopril and ethanol had no effect. LDL incubated with captopril showed only 13.8% of TBARS and 22.7% of lipid peroxides produced by control (100%) after 1 h. Increasing concentrations of captopril showed a linear increase in the lag phase. We conclude that captopril increases the resistance of LDL to copper-induced oxidation.},
  author       = {Zieden, B and Wuttge, Dirk and Karlberg, B E and Olsson, A G},
  issn         = {1365-2125},
  language     = {eng},
  number       = {2},
  pages        = {201--203},
  publisher    = {John Wiley & Sons},
  series       = {British Journal of Clinical Pharmacology},
  title        = {Effects of in vitro addition of captopril on copper-induced low density lipoprotein oxidation},
  volume       = {39},
  year         = {1995},
}