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Influence of an enriched environment and cortical grafting on functional outcome in brain infarcts of adult rats

Grabowski, Martin LU ; Sorensen, Jens Christian; Mattsson, Bengt LU ; Zimmer, Jens and Johansson, Barbro LU (1995) In Experimental Neurology 133(1). p.96-102
Abstract
The purpose of this work was to study if enriched housing conditions and fetal neocortical transplantation could enhance the functional outcome after focal brain ischemia in adult rats. The right middle cerebral artery (MCA) was ligated in 34 inbred, spontaneously hypertensive male rats, which were then randomly divided into three groups. Groups A and B were transferred to an enriched environment, i.e., a large cage with opportunities for various activities but not forcing the rats to do any particular tasks; group C was kept in standard laboratory cages. Three weeks after the MCA occlusion blocks of fetal neocortical tissue (Embryonic Day 17) were transplanted to the infarct cavity in groups B and C. Rats in group A (n = 11) and group B... (More)
The purpose of this work was to study if enriched housing conditions and fetal neocortical transplantation could enhance the functional outcome after focal brain ischemia in adult rats. The right middle cerebral artery (MCA) was ligated in 34 inbred, spontaneously hypertensive male rats, which were then randomly divided into three groups. Groups A and B were transferred to an enriched environment, i.e., a large cage with opportunities for various activities but not forcing the rats to do any particular tasks; group C was kept in standard laboratory cages. Three weeks after the MCA occlusion blocks of fetal neocortical tissue (Embryonic Day 17) were transplanted to the infarct cavity in groups B and C. Rats in group A (n = 11) and group B (n = 11) performed equally well and significantly better than rats in group C (n = 10) when placed on an inclined plane and when traversing a rotating pole 6 and 9 weeks after the MCA occlusion and in a leg placement test at 9, but not 6 and 12 weeks. Skilled forelimb function did not differ between the groups. Infarct size and thalamic atrophy did not differ between the groups and graft size was similar in group B and C. There was no correlation between infarct size and motor function in any of the tests in rats housed in an enriched environment. Since the environment can significantly alter functional outcome without reducing infarct size we suggest that more attention should be given to the role of the laboratory environment and to long term behavioral outcome in experimental stroke. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Neurology
volume
133
issue
1
pages
96 - 102
publisher
Academic Press
external identifiers
  • pmid:7601267
  • scopus:0029070781
ISSN
0014-4886
DOI
10.1006/exnr.1995.1011
language
English
LU publication?
yes
id
d7a5f01a-9c64-4fd8-8461-a52195fe26ba (old id 1109337)
date added to LUP
2008-07-28 10:04:30
date last changed
2017-08-06 03:41:03
@article{d7a5f01a-9c64-4fd8-8461-a52195fe26ba,
  abstract     = {The purpose of this work was to study if enriched housing conditions and fetal neocortical transplantation could enhance the functional outcome after focal brain ischemia in adult rats. The right middle cerebral artery (MCA) was ligated in 34 inbred, spontaneously hypertensive male rats, which were then randomly divided into three groups. Groups A and B were transferred to an enriched environment, i.e., a large cage with opportunities for various activities but not forcing the rats to do any particular tasks; group C was kept in standard laboratory cages. Three weeks after the MCA occlusion blocks of fetal neocortical tissue (Embryonic Day 17) were transplanted to the infarct cavity in groups B and C. Rats in group A (n = 11) and group B (n = 11) performed equally well and significantly better than rats in group C (n = 10) when placed on an inclined plane and when traversing a rotating pole 6 and 9 weeks after the MCA occlusion and in a leg placement test at 9, but not 6 and 12 weeks. Skilled forelimb function did not differ between the groups. Infarct size and thalamic atrophy did not differ between the groups and graft size was similar in group B and C. There was no correlation between infarct size and motor function in any of the tests in rats housed in an enriched environment. Since the environment can significantly alter functional outcome without reducing infarct size we suggest that more attention should be given to the role of the laboratory environment and to long term behavioral outcome in experimental stroke.},
  author       = {Grabowski, Martin and Sorensen, Jens Christian and Mattsson, Bengt and Zimmer, Jens and Johansson, Barbro},
  issn         = {0014-4886},
  language     = {eng},
  number       = {1},
  pages        = {96--102},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Influence of an enriched environment and cortical grafting on functional outcome in brain infarcts of adult rats},
  url          = {http://dx.doi.org/10.1006/exnr.1995.1011},
  volume       = {133},
  year         = {1995},
}