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Long-term preservation of vascular endothelium and smooth muscle

Ingemansson, Richard LU ; Sjöberg, Trygve LU ; Massa, Giorgio and Steen, Stig LU (1995) In Annals of Thoracic Surgery 59(5). p.1177-1181
Abstract
This study was performed in organ baths on 400 ring segments of infrarenal aorta taken from 40 Sprague-Dawley rats that had been randomized into five groups. Contractility was tested with the thromboxane analogue U-46619. Acetylcholine was used to elicit endothelium-dependent relaxing factor (EDRF). The results obtained from vessels preserved at 4 degrees C for 6, 12, 24, and 36 hours were compared with those from autologous vessels studied immediately after harvesting. Vessels preserved in Euro-Collins solution showed a 46% (p < 0.01) decrease in contractility after 12 hours of storage; after 24 hours only weak contractions could be elicited, and after 36 hours they had lost their ability to contract. The EDRF function was slightly... (More)
This study was performed in organ baths on 400 ring segments of infrarenal aorta taken from 40 Sprague-Dawley rats that had been randomized into five groups. Contractility was tested with the thromboxane analogue U-46619. Acetylcholine was used to elicit endothelium-dependent relaxing factor (EDRF). The results obtained from vessels preserved at 4 degrees C for 6, 12, 24, and 36 hours were compared with those from autologous vessels studied immediately after harvesting. Vessels preserved in Euro-Collins solution showed a 46% (p < 0.01) decrease in contractility after 12 hours of storage; after 24 hours only weak contractions could be elicited, and after 36 hours they had lost their ability to contract. The EDRF function was slightly reduced after 12 hours and could not be investigated after 24 and 36 hours. With the University of Wisconsin solution (UW) and the low-potassium-dextran-glucose solution Perfadex no decrease in contractility was seen in the first 24 hours, but at 36 hours the vessels preserved in UW had lost 40% (p < 0.01) and those preserved in Perfadex 30% (p < 0.05) of their contractility. The EDRF function was significantly reduced by about 15% after 6, 12, and 24 hours in both the UW and the Perfadex groups. At 36 hours, vessels stored in Perfadex had lost 41% (p < 0.001) and those stored in UW 17% (p < 0.01) of their EDRF function.(ABSTRACT TRUNCATED AT 250 WORDS) (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Annals of Thoracic Surgery
volume
59
issue
5
pages
1177 - 1181
publisher
Elsevier
external identifiers
  • pmid:7733716
  • scopus:0028908398
ISSN
1552-6259
DOI
10.1016/0003-4975(95)00126-6
language
English
LU publication?
yes
id
80e756d2-86a4-45be-9558-10b390660a9d (old id 1109678)
date added to LUP
2008-07-29 13:05:35
date last changed
2017-01-01 04:48:05
@article{80e756d2-86a4-45be-9558-10b390660a9d,
  abstract     = {This study was performed in organ baths on 400 ring segments of infrarenal aorta taken from 40 Sprague-Dawley rats that had been randomized into five groups. Contractility was tested with the thromboxane analogue U-46619. Acetylcholine was used to elicit endothelium-dependent relaxing factor (EDRF). The results obtained from vessels preserved at 4 degrees C for 6, 12, 24, and 36 hours were compared with those from autologous vessels studied immediately after harvesting. Vessels preserved in Euro-Collins solution showed a 46% (p &lt; 0.01) decrease in contractility after 12 hours of storage; after 24 hours only weak contractions could be elicited, and after 36 hours they had lost their ability to contract. The EDRF function was slightly reduced after 12 hours and could not be investigated after 24 and 36 hours. With the University of Wisconsin solution (UW) and the low-potassium-dextran-glucose solution Perfadex no decrease in contractility was seen in the first 24 hours, but at 36 hours the vessels preserved in UW had lost 40% (p &lt; 0.01) and those preserved in Perfadex 30% (p &lt; 0.05) of their contractility. The EDRF function was significantly reduced by about 15% after 6, 12, and 24 hours in both the UW and the Perfadex groups. At 36 hours, vessels stored in Perfadex had lost 41% (p &lt; 0.001) and those stored in UW 17% (p &lt; 0.01) of their EDRF function.(ABSTRACT TRUNCATED AT 250 WORDS)},
  author       = {Ingemansson, Richard and Sjöberg, Trygve and Massa, Giorgio and Steen, Stig},
  issn         = {1552-6259},
  language     = {eng},
  number       = {5},
  pages        = {1177--1181},
  publisher    = {Elsevier},
  series       = {Annals of Thoracic Surgery},
  title        = {Long-term preservation of vascular endothelium and smooth muscle},
  url          = {http://dx.doi.org/10.1016/0003-4975(95)00126-6},
  volume       = {59},
  year         = {1995},
}