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Structural organization, expression and chromosomal mapping of the mouse cystatin C gene (Cst3)

Huh, Changgoo; Nagle, James W; Kozak, Christine A; Abrahamson, Magnus LU and Karlsson, Stefan (1995) In Gene 152(2). p.221-226
Abstract
Cystatin C (CstC) is a potent cysteine-proteinase inhibitor. The structure of the mouse CstC-encoding gene (Cst3) was examined by sequencing a 6.1-kb genomic DNA containing the entire gene, as well as 0.9 kb of 5′ flanking and 1.7 kb of its 3′ flanking region. The sequence revealed that the overall organization of the gene is very similar to those of the genes encoding human CstC and other type-2 Cst, with two introns at positions identical to those in the human gene. The promoter area does not contain typical TATA or CAAT boxes. Two copies of a Spl-binding motif, GGGCGG, are present in the 5′ flanking region within 300 bp upstream from the initiation codon. A hexa-nucleotide, TGTTCT, which is a core sequence of the androgen-responsive... (More)
Cystatin C (CstC) is a potent cysteine-proteinase inhibitor. The structure of the mouse CstC-encoding gene (Cst3) was examined by sequencing a 6.1-kb genomic DNA containing the entire gene, as well as 0.9 kb of 5′ flanking and 1.7 kb of its 3′ flanking region. The sequence revealed that the overall organization of the gene is very similar to those of the genes encoding human CstC and other type-2 Cst, with two introns at positions identical to those in the human gene. The promoter area does not contain typical TATA or CAAT boxes. Two copies of a Spl-binding motif, GGGCGG, are present in the 5′ flanking region within 300 bp upstream from the initiation codon. A hexa-nucleotide, TGTTCT, which is a core sequence of the androgen-responsive element (ARE), is found in the promoter region. This region also contains a 21-nucleotide sequence, 5′-AGACTAGCAGCTGACTGAAGC, which contains two potential binding sites for the transcription factor, AP-1. The mouse Cst3 mRNA was detected in all of thirteen tissues examined by Northern blot analysis. Cst3 was mapped in the mouse to a position on distal chromosome 2. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Recombinant genomic DNA, gene mapping, exon, intron, promoter sequence, proteinase inhibitor, multigene family
in
Gene
volume
152
issue
2
pages
221 - 226
publisher
Elsevier
external identifiers
  • scopus:0028939368
ISSN
1879-0038
DOI
10.1016/0378-1119(94)00728-B
language
English
LU publication?
yes
id
e78b3eeb-d0e6-4eae-b2ea-bdf79529282d (old id 1109715)
date added to LUP
2008-07-29 14:24:30
date last changed
2017-06-11 04:34:31
@article{e78b3eeb-d0e6-4eae-b2ea-bdf79529282d,
  abstract     = {Cystatin C (CstC) is a potent cysteine-proteinase inhibitor. The structure of the mouse CstC-encoding gene (Cst3) was examined by sequencing a 6.1-kb genomic DNA containing the entire gene, as well as 0.9 kb of 5′ flanking and 1.7 kb of its 3′ flanking region. The sequence revealed that the overall organization of the gene is very similar to those of the genes encoding human CstC and other type-2 Cst, with two introns at positions identical to those in the human gene. The promoter area does not contain typical TATA or CAAT boxes. Two copies of a Spl-binding motif, GGGCGG, are present in the 5′ flanking region within 300 bp upstream from the initiation codon. A hexa-nucleotide, TGTTCT, which is a core sequence of the androgen-responsive element (ARE), is found in the promoter region. This region also contains a 21-nucleotide sequence, 5′-AGACTAGCAGCTGACTGAAGC, which contains two potential binding sites for the transcription factor, AP-1. The mouse Cst3 mRNA was detected in all of thirteen tissues examined by Northern blot analysis. Cst3 was mapped in the mouse to a position on distal chromosome 2.},
  author       = {Huh, Changgoo and Nagle, James W and Kozak, Christine A and Abrahamson, Magnus and Karlsson, Stefan},
  issn         = {1879-0038},
  keyword      = {Recombinant genomic DNA,gene mapping,exon,intron,promoter sequence,proteinase inhibitor,multigene family},
  language     = {eng},
  number       = {2},
  pages        = {221--226},
  publisher    = {Elsevier},
  series       = {Gene},
  title        = {Structural organization, expression and chromosomal mapping of the mouse cystatin C gene (Cst3)},
  url          = {http://dx.doi.org/10.1016/0378-1119(94)00728-B},
  volume       = {152},
  year         = {1995},
}