Epithelial pathways for luminal entry of bulk plasma
(1995) In Clinical and Experimental Allergy 25(2). p.187-195- Abstract
- Inflammatory challenges of the airway mucosa cause luminal entry of bulk plasma. Extravasation of plasma is well described but the routes for epithelial passage of plasma are largely unknown. Using colloidal gold (5 nm) as tracer we have now examined the fate of extravasated plasma in the airways. The tracer was given intravenously to anaesthetized, ovalbumin-sensitized guinea-pigs 2min prior to airway mucosal challenge with 12pmol ovalbumin (the dose was selected from a separate dose-response study). Tissue specimens were collected 30s, 3 and 6 min after end of challenge (separate time course experiments suggested that the peak rate of entry of plasma occurred at about 5 min). The colloidal gold particles were visualized by... (More)
- Inflammatory challenges of the airway mucosa cause luminal entry of bulk plasma. Extravasation of plasma is well described but the routes for epithelial passage of plasma are largely unknown. Using colloidal gold (5 nm) as tracer we have now examined the fate of extravasated plasma in the airways. The tracer was given intravenously to anaesthetized, ovalbumin-sensitized guinea-pigs 2min prior to airway mucosal challenge with 12pmol ovalbumin (the dose was selected from a separate dose-response study). Tissue specimens were collected 30s, 3 and 6 min after end of challenge (separate time course experiments suggested that the peak rate of entry of plasma occurred at about 5 min). The colloidal gold particles were visualized by autometallographic silver intensification. The gold produced no circulatory disturbance and had a uniform vascular distribution with negligible adherence to vascular endothelium. After challenge gold was first widely distributed in the lamina propria. At 3 and 6 min the tracer was also in the epithelium and airway lumen. It appeared that plasma was moved distinctly between and all around each epithelial cell. Bright field-, scanning-, and transmission electron-microscopy indicated that the luminal entry of plasma did not affect the integrity of the epithelial lining. This study demonstrates that the plasma exudate moves across an intact epithelial layer through ubiquitous paracellular pathways. Even at a pronounced acute plasma exudation response exceedingly small amounts of plasma may pass around a single cell explaining the non-injurious nature of mucosal exudation of bulk plasma in health and disease. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1109760
- author
- Erjefält, Jonas LU ; Erjefalt, Ingrid ; Sundler, Frank LU and Persson, Carl LU
- organization
- publishing date
- 1995
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical and Experimental Allergy
- volume
- 25
- issue
- 2
- pages
- 187 - 195
- publisher
- Wiley
- external identifiers
-
- pmid:7750011
- scopus:0028967957
- ISSN
- 1365-2222
- DOI
- 10.1111/j.1365-2222.1995.tb01025.x
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Clinical Chemistry and Pharmacology (013250300), Neuroendocrine Cell Biology (013212008), Airway Inflammation and Immunology (013212038)
- id
- 49b2bb06-78e8-462e-82a4-9720d520c10f (old id 1109760)
- date added to LUP
- 2016-04-01 11:49:58
- date last changed
- 2025-04-04 13:55:24
@article{49b2bb06-78e8-462e-82a4-9720d520c10f,
abstract = {{Inflammatory challenges of the airway mucosa cause luminal entry of bulk plasma. Extravasation of plasma is well described but the routes for epithelial passage of plasma are largely unknown. Using colloidal gold (5 nm) as tracer we have now examined the fate of extravasated plasma in the airways. The tracer was given intravenously to anaesthetized, ovalbumin-sensitized guinea-pigs 2min prior to airway mucosal challenge with 12pmol ovalbumin (the dose was selected from a separate dose-response study). Tissue specimens were collected 30s, 3 and 6 min after end of challenge (separate time course experiments suggested that the peak rate of entry of plasma occurred at about 5 min). The colloidal gold particles were visualized by autometallographic silver intensification. The gold produced no circulatory disturbance and had a uniform vascular distribution with negligible adherence to vascular endothelium. After challenge gold was first widely distributed in the lamina propria. At 3 and 6 min the tracer was also in the epithelium and airway lumen. It appeared that plasma was moved distinctly between and all around each epithelial cell. Bright field-, scanning-, and transmission electron-microscopy indicated that the luminal entry of plasma did not affect the integrity of the epithelial lining. This study demonstrates that the plasma exudate moves across an intact epithelial layer through ubiquitous paracellular pathways. Even at a pronounced acute plasma exudation response exceedingly small amounts of plasma may pass around a single cell explaining the non-injurious nature of mucosal exudation of bulk plasma in health and disease.}},
author = {{Erjefält, Jonas and Erjefalt, Ingrid and Sundler, Frank and Persson, Carl}},
issn = {{1365-2222}},
language = {{eng}},
number = {{2}},
pages = {{187--195}},
publisher = {{Wiley}},
series = {{Clinical and Experimental Allergy}},
title = {{Epithelial pathways for luminal entry of bulk plasma}},
url = {{http://dx.doi.org/10.1111/j.1365-2222.1995.tb01025.x}},
doi = {{10.1111/j.1365-2222.1995.tb01025.x}},
volume = {{25}},
year = {{1995}},
}