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Regulation of phospholipase D activity in neuroblastoma cells

Gustavsson, Lena; Boyano-Adanez, Maria del Carmen; Larsson, Christer LU ; Aradottir, Steina LU and Lundqvist, Cristofer (1996) In Journal of Lipid Mediators and Cell Signalling 14(1-3). p.229-235
Abstract
The regulation of phospholipase D was studied in human neuroblastoma cells using phosphatidylethanol as a marker of the enzyme activity. Carbachol induced phospholipase D activity in SH-SY5Y cells. Muscarinic antagonists inhibited the response with potencies suggesting that muscarinic M1 receptors are responsible for the activation. In permeabilized SH-SY5Y cells, both the carbachol- and GTP gamma S-induced Peth formation was inhibited by GDP beta S, indicating that both responses are mediated via a G-protein. The protein kinase C inhibitors, bisindolylmaleimide and staurosporine significantly inhibited the carbachol-induced Peth formation whereas H7 had no effect. Thus, the cholinergic activation of phospholipase D in SH-SY5Y cells is... (More)
The regulation of phospholipase D was studied in human neuroblastoma cells using phosphatidylethanol as a marker of the enzyme activity. Carbachol induced phospholipase D activity in SH-SY5Y cells. Muscarinic antagonists inhibited the response with potencies suggesting that muscarinic M1 receptors are responsible for the activation. In permeabilized SH-SY5Y cells, both the carbachol- and GTP gamma S-induced Peth formation was inhibited by GDP beta S, indicating that both responses are mediated via a G-protein. The protein kinase C inhibitors, bisindolylmaleimide and staurosporine significantly inhibited the carbachol-induced Peth formation whereas H7 had no effect. Thus, the cholinergic activation of phospholipase D in SH-SY5Y cells is probably mediated via a direct receptor-G-protein coupling but an involvement of protein kinase C cannot be excluded. Calmidazolium, a calmodulin antagonist, induced an increase in phosphatidylethanol formation in both SH-SY5Y and IMR-32 cells. This effect was inhibited by genistein and tyrphostin, indicating a tyrosine kinase dependent pathway for phospholipase D activation in neuroblastoma cells. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Calmidazolium, Muscarinic receptors, Phosphatidylethanol, Phosphatidic acid, Phospholipase D, Signal transduction
in
Journal of Lipid Mediators and Cell Signalling
volume
14
issue
1-3
pages
229 - 235
publisher
Elsevier
external identifiers
  • pmid:8906567
  • scopus:0030238946
ISSN
0929-7855
DOI
10.1016/0929-7855(96)00530-5
language
English
LU publication?
yes
id
dda7f951-3e01-48bc-993d-998f9669ea9a (old id 1109867)
date added to LUP
2008-07-23 10:49:55
date last changed
2017-01-01 06:45:36
@article{dda7f951-3e01-48bc-993d-998f9669ea9a,
  abstract     = {The regulation of phospholipase D was studied in human neuroblastoma cells using phosphatidylethanol as a marker of the enzyme activity. Carbachol induced phospholipase D activity in SH-SY5Y cells. Muscarinic antagonists inhibited the response with potencies suggesting that muscarinic M1 receptors are responsible for the activation. In permeabilized SH-SY5Y cells, both the carbachol- and GTP gamma S-induced Peth formation was inhibited by GDP beta S, indicating that both responses are mediated via a G-protein. The protein kinase C inhibitors, bisindolylmaleimide and staurosporine significantly inhibited the carbachol-induced Peth formation whereas H7 had no effect. Thus, the cholinergic activation of phospholipase D in SH-SY5Y cells is probably mediated via a direct receptor-G-protein coupling but an involvement of protein kinase C cannot be excluded. Calmidazolium, a calmodulin antagonist, induced an increase in phosphatidylethanol formation in both SH-SY5Y and IMR-32 cells. This effect was inhibited by genistein and tyrphostin, indicating a tyrosine kinase dependent pathway for phospholipase D activation in neuroblastoma cells.},
  author       = {Gustavsson, Lena and Boyano-Adanez, Maria del Carmen and Larsson, Christer and Aradottir, Steina and Lundqvist, Cristofer},
  issn         = {0929-7855},
  keyword      = {Calmidazolium,Muscarinic receptors,Phosphatidylethanol,Phosphatidic acid,Phospholipase D,Signal transduction},
  language     = {eng},
  number       = {1-3},
  pages        = {229--235},
  publisher    = {Elsevier},
  series       = {Journal of Lipid Mediators and Cell Signalling},
  title        = {Regulation of phospholipase D activity in neuroblastoma cells},
  url          = {http://dx.doi.org/10.1016/0929-7855(96)00530-5},
  volume       = {14},
  year         = {1996},
}