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Early B-Cell Factor, E2A, and Pax-5 Cooperate To Activate the Early B Cell-Specific mb-1 Promoter.

Sigvardsson, Mikael LU ; Clark, Dawn R; Fitzsimmons, Daniel; Doyle, Michelle; Åkerblad, Peter; Breslin, Thomas; Bilke, Sven; Li, Ronggui; Yeamans, Carmen and Zhang, Gongyi, et al. (2002) In Molecular and Cellular Biology 22(24). p.8539-8551
Abstract
Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets... (More)
Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets ternary complex partners) are required for promoter function in all mb-1-expressing cells. Using DNA microarrays, we found that expression of endogenous mb-1 transcripts correlates most closely with EBF expression and negatively with Id1, an inhibitor of E2A protein function, further linking regulation of the mb-1 gene with EBF and E2A. Together, our studies demonstrate the complexity of factors regulating tissue-specific transcription and support the concept that EBF, E2A, and Pax-5 cooperate to activate target genes in early B-cell development. (Less)
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Molecular and Cellular Biology
volume
22
issue
24
pages
8539 - 8551
publisher
American Society for Microbiology
external identifiers
  • wos:000179527400015
  • scopus:18744388827
ISSN
0270-7306
DOI
10.1128/MCB.22.24.8539-8551.2002
language
English
LU publication?
yes
id
ed504acb-3db1-44b2-bf75-600d26aa2d00 (old id 110995)
date added to LUP
2007-07-20 12:50:28
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2017-11-19 03:34:37
@article{ed504acb-3db1-44b2-bf75-600d26aa2d00,
  abstract     = {Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets ternary complex partners) are required for promoter function in all mb-1-expressing cells. Using DNA microarrays, we found that expression of endogenous mb-1 transcripts correlates most closely with EBF expression and negatively with Id1, an inhibitor of E2A protein function, further linking regulation of the mb-1 gene with EBF and E2A. Together, our studies demonstrate the complexity of factors regulating tissue-specific transcription and support the concept that EBF, E2A, and Pax-5 cooperate to activate target genes in early B-cell development.},
  author       = {Sigvardsson, Mikael and Clark, Dawn R and Fitzsimmons, Daniel and Doyle, Michelle and Åkerblad, Peter and Breslin, Thomas and Bilke, Sven and Li, Ronggui and Yeamans, Carmen and Zhang, Gongyi and Hagman, James},
  issn         = {0270-7306},
  language     = {eng},
  number       = {24},
  pages        = {8539--8551},
  publisher    = {American Society for Microbiology},
  series       = {Molecular and Cellular Biology},
  title        = {Early B-Cell Factor, E2A, and Pax-5 Cooperate To Activate the Early B Cell-Specific mb-1 Promoter.},
  url          = {http://dx.doi.org/10.1128/MCB.22.24.8539-8551.2002},
  volume       = {22},
  year         = {2002},
}