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Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method

Garkavij, Michael LU ; Tennvall, Jan LU ; Strand, Sven-Erik LU ; Nilsson, Rune LU ; Lindgren, Lars; Chen, Jangin; Isaksson, Martin; Eriksson, Håkan and Sjögren, Hans-Olof (1996) In Acta Oncologica 35(3). p.309-312
Abstract
This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any... (More)
This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Oncologica
volume
35
issue
3
pages
309 - 312
publisher
Taylor & Francis
external identifiers
  • pmid:8679261
  • scopus:0029894616
ISSN
1651-226X
DOI
10.3109/02841869609101646
language
English
LU publication?
yes
id
10c55935-bd9c-4c15-a855-ac5c41dcf476 (old id 1110285)
date added to LUP
2008-07-23 10:09:40
date last changed
2017-01-01 06:54:54
@article{10c55935-bd9c-4c15-a855-ac5c41dcf476,
  abstract     = {This study of 36 rats with rat colon adenocarcinoma transplants was carried out to investigate the efficacy of a new method of whole blood immunoadsorption (WBIA) in removing biotinylated monoclonal antibodies (MAbs) directly from unseparated blood, in order to increase 'the tumor/normal-tissue uptake ratio', as compared with extracorporeal immunoadsorption (ECIA) of antibodies from plasma. Compared with the ECIA system, the overall volume of the WBIA system (comprising only a pump, an adsorption column, a drop-chamber and tubings) was less (3.6 vs. 6.2 ml), and procedure duration 2 h less. The 17 rats undergoing the WBIA procedure, started 12 h after i.v. injection of 4.0-4.5 MBq 125I-BR96-biotin, manifested neither hemolysis nor any other complication; no signs of organ edema were found at dissection; whole body and blood radioactivity values were reduced by 51% and 89.5%, respectively. The WBIA method was as effective as ECIA, but technically simpler, safer and more reliable.},
  author       = {Garkavij, Michael and Tennvall, Jan and Strand, Sven-Erik and Nilsson, Rune and Lindgren, Lars and Chen, Jangin and Isaksson, Martin and Eriksson, Håkan and Sjögren, Hans-Olof},
  issn         = {1651-226X},
  language     = {eng},
  number       = {3},
  pages        = {309--312},
  publisher    = {Taylor & Francis},
  series       = {Acta Oncologica},
  title        = {Extracorporeal immunoadsorption from whole blood based on the avidin-biotin concept. Evaluation of a new method},
  url          = {http://dx.doi.org/10.3109/02841869609101646},
  volume       = {35},
  year         = {1996},
}