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Bone graft proteins influence osteoconduction. A titanium chamber study in rats

Aspenberg, Per LU ; Tägil, Magnus LU ; Kristensson, Carina and Lidin, Sven (1996) In Acta Orthopaedica Scandinavica 67(4). p.377-382
Abstract
Although it is often emphasized that the matrix of bone grafts contains several growth factors, it is not known if these factors become activated and play a role in bone grafting. We therefore compared ground defatted bone which had or had not been deproteinized by heating with water to 270 degrees C at an autogenic pressure of 55 bar. This is a careful ceramic procedure which leaves the mineral unchanged. Deproteinized and non-deproteinized bone granulae derived from cortical rat bone were placed in titanium bone conduction chambers implanted bilaterally in rat tibiae. Ingrowing bone could enter the cylindrical interior of the chamber only at one end. It then penetrated the material in the chamber, but due to the length of the cylinder,... (More)
Although it is often emphasized that the matrix of bone grafts contains several growth factors, it is not known if these factors become activated and play a role in bone grafting. We therefore compared ground defatted bone which had or had not been deproteinized by heating with water to 270 degrees C at an autogenic pressure of 55 bar. This is a careful ceramic procedure which leaves the mineral unchanged. Deproteinized and non-deproteinized bone granulae derived from cortical rat bone were placed in titanium bone conduction chambers implanted bilaterally in rat tibiae. Ingrowing bone could enter the cylindrical interior of the chamber only at one end. It then penetrated the material in the chamber, but due to the length of the cylinder, it never reached the other end. The mean distance which the ingrown bone had reached in the material was then measured on histological slides. The bone formation activity was measured by TcMDP scintimetry. With the protein-containing granulae, the mean bone ingrowth distance and the scintimetric activity were increased by 41% and 31%, respectively (p < 0.01). We conclude that there is a limited favourable effect of proteins in a graft. Our grounded material had a large fracture surface area with no osteoid lining. The leakage of growth factors from such areas may explain the extraordinarily good clinical incorporation of morselized compacted allografts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Orthopaedica Scandinavica
volume
67
issue
4
pages
377 - 382
publisher
Taylor & Francis
external identifiers
  • pmid:8792743
  • scopus:0029825176
ISSN
0001-6470
DOI
10.3109/17453679609002335
language
English
LU publication?
yes
id
90d3346c-b7c5-4aa4-a299-a76bc1460d80 (old id 1110294)
date added to LUP
2008-07-21 10:44:06
date last changed
2017-01-01 07:01:54
@article{90d3346c-b7c5-4aa4-a299-a76bc1460d80,
  abstract     = {Although it is often emphasized that the matrix of bone grafts contains several growth factors, it is not known if these factors become activated and play a role in bone grafting. We therefore compared ground defatted bone which had or had not been deproteinized by heating with water to 270 degrees C at an autogenic pressure of 55 bar. This is a careful ceramic procedure which leaves the mineral unchanged. Deproteinized and non-deproteinized bone granulae derived from cortical rat bone were placed in titanium bone conduction chambers implanted bilaterally in rat tibiae. Ingrowing bone could enter the cylindrical interior of the chamber only at one end. It then penetrated the material in the chamber, but due to the length of the cylinder, it never reached the other end. The mean distance which the ingrown bone had reached in the material was then measured on histological slides. The bone formation activity was measured by TcMDP scintimetry. With the protein-containing granulae, the mean bone ingrowth distance and the scintimetric activity were increased by 41% and 31%, respectively (p &lt; 0.01). We conclude that there is a limited favourable effect of proteins in a graft. Our grounded material had a large fracture surface area with no osteoid lining. The leakage of growth factors from such areas may explain the extraordinarily good clinical incorporation of morselized compacted allografts.},
  author       = {Aspenberg, Per and Tägil, Magnus and Kristensson, Carina and Lidin, Sven},
  issn         = {0001-6470},
  language     = {eng},
  number       = {4},
  pages        = {377--382},
  publisher    = {Taylor & Francis},
  series       = {Acta Orthopaedica Scandinavica},
  title        = {Bone graft proteins influence osteoconduction. A titanium chamber study in rats},
  url          = {http://dx.doi.org/10.3109/17453679609002335},
  volume       = {67},
  year         = {1996},
}