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Neuropeptide Y potentiates noradrenaline-induced contraction through the neuropeptide Y Y1 receptor

Bergdahl, Anders; Nilsson, Torun; Cantera, Leonor; Nilsson, Leif; Sun, Xiang-Ying; Hedner, Tomas; Erlinge, David LU ; Valdemarson, Stig and Edvinsson, Lars LU (1996) In European Journal of Pharmacology 316(1). p.59-64
Abstract
To elucidate which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation of the noradrenaline-evoked contraction in human omental arteries we used antisense oligodeoxynucleotide (Antisense), the new selective neuropeptide Y Y1 receptor antagonist, BIBP3226 {(R)-N2-(diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine-amide} and the reverse transcriptase-polymerase chain reaction (RT-PCR). Neuropeptide Y significantly potentiated the noradrenaline-induced contraction in non-incubated vessels (pEC50 6.4 +/- 0.2 vs. 5.9 +/- 0.2) and in vessels incubated with 1 microM Sense oligodeoxynucleotide (Sense) (pEC50 6.0 +/- 0.1 vs. 5.6 +/- 0.2). In vessels incubated with 1 microM Antisense the potentiating... (More)
To elucidate which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation of the noradrenaline-evoked contraction in human omental arteries we used antisense oligodeoxynucleotide (Antisense), the new selective neuropeptide Y Y1 receptor antagonist, BIBP3226 {(R)-N2-(diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine-amide} and the reverse transcriptase-polymerase chain reaction (RT-PCR). Neuropeptide Y significantly potentiated the noradrenaline-induced contraction in non-incubated vessels (pEC50 6.4 +/- 0.2 vs. 5.9 +/- 0.2) and in vessels incubated with 1 microM Sense oligodeoxynucleotide (Sense) (pEC50 6.0 +/- 0.1 vs. 5.6 +/- 0.2). In vessels incubated with 1 microM Antisense the potentiating effect of neuropeptide Y was completely abolished. BIBP3226 (1 microM) inhibited the neuropeptide Y-induced potentiation in human omental arteries (pEC50 5.8 +/- 0.3 vs. 6.4 +/- 0.2). Finally, messenger RNA for the neuropeptide Y Y1 receptor was detected using RT-PCR. On the basis of our results we conclude that the neuropeptide Y-induced potentiation of the noradrenaline-induced contraction is mediated by the neuropeptide Y Y1 receptor. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Artery, human, Noradrenaline, Neuropeptide Y, Antisense oligodeoxynucleotide, BIBP3226, Reverse transcriptase-polymerase chain reaction
in
European Journal of Pharmacology
volume
316
issue
1
pages
59 - 64
publisher
Elsevier
external identifiers
  • pmid:8982651
  • scopus:0030605216
ISSN
1879-0712
DOI
10.1016/S0014-2999(96)00636-X
language
English
LU publication?
yes
id
c2c43d90-2ce2-401a-b8a0-a21abb49b4c3 (old id 1110302)
date added to LUP
2008-07-21 11:19:46
date last changed
2017-01-01 04:46:00
@article{c2c43d90-2ce2-401a-b8a0-a21abb49b4c3,
  abstract     = {To elucidate which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation of the noradrenaline-evoked contraction in human omental arteries we used antisense oligodeoxynucleotide (Antisense), the new selective neuropeptide Y Y1 receptor antagonist, BIBP3226 {(R)-N2-(diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine-amide} and the reverse transcriptase-polymerase chain reaction (RT-PCR). Neuropeptide Y significantly potentiated the noradrenaline-induced contraction in non-incubated vessels (pEC50 6.4 +/- 0.2 vs. 5.9 +/- 0.2) and in vessels incubated with 1 microM Sense oligodeoxynucleotide (Sense) (pEC50 6.0 +/- 0.1 vs. 5.6 +/- 0.2). In vessels incubated with 1 microM Antisense the potentiating effect of neuropeptide Y was completely abolished. BIBP3226 (1 microM) inhibited the neuropeptide Y-induced potentiation in human omental arteries (pEC50 5.8 +/- 0.3 vs. 6.4 +/- 0.2). Finally, messenger RNA for the neuropeptide Y Y1 receptor was detected using RT-PCR. On the basis of our results we conclude that the neuropeptide Y-induced potentiation of the noradrenaline-induced contraction is mediated by the neuropeptide Y Y1 receptor.},
  author       = {Bergdahl, Anders and Nilsson, Torun and Cantera, Leonor and Nilsson, Leif and Sun, Xiang-Ying and Hedner, Tomas and Erlinge, David and Valdemarson, Stig and Edvinsson, Lars},
  issn         = {1879-0712},
  keyword      = {Artery,human,Noradrenaline,Neuropeptide Y,Antisense oligodeoxynucleotide,BIBP3226,Reverse transcriptase-polymerase chain reaction},
  language     = {eng},
  number       = {1},
  pages        = {59--64},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Neuropeptide Y potentiates noradrenaline-induced contraction through the neuropeptide Y Y1 receptor},
  url          = {http://dx.doi.org/10.1016/S0014-2999(96)00636-X},
  volume       = {316},
  year         = {1996},
}