Neuropeptide Y potentiates noradrenaline-induced contraction through the neuropeptide Y Y1 receptor
(1996) In European Journal of Pharmacology 316(1). p.59-64- Abstract
- To elucidate which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation of the noradrenaline-evoked contraction in human omental arteries we used antisense oligodeoxynucleotide (Antisense), the new selective neuropeptide Y Y1 receptor antagonist, BIBP3226 {(R)-N2-(diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine-amide} and the reverse transcriptase-polymerase chain reaction (RT-PCR). Neuropeptide Y significantly potentiated the noradrenaline-induced contraction in non-incubated vessels (pEC50 6.4 +/- 0.2 vs. 5.9 +/- 0.2) and in vessels incubated with 1 microM Sense oligodeoxynucleotide (Sense) (pEC50 6.0 +/- 0.1 vs. 5.6 +/- 0.2). In vessels incubated with 1 microM Antisense the potentiating... (More)
- To elucidate which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation of the noradrenaline-evoked contraction in human omental arteries we used antisense oligodeoxynucleotide (Antisense), the new selective neuropeptide Y Y1 receptor antagonist, BIBP3226 {(R)-N2-(diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine-amide} and the reverse transcriptase-polymerase chain reaction (RT-PCR). Neuropeptide Y significantly potentiated the noradrenaline-induced contraction in non-incubated vessels (pEC50 6.4 +/- 0.2 vs. 5.9 +/- 0.2) and in vessels incubated with 1 microM Sense oligodeoxynucleotide (Sense) (pEC50 6.0 +/- 0.1 vs. 5.6 +/- 0.2). In vessels incubated with 1 microM Antisense the potentiating effect of neuropeptide Y was completely abolished. BIBP3226 (1 microM) inhibited the neuropeptide Y-induced potentiation in human omental arteries (pEC50 5.8 +/- 0.3 vs. 6.4 +/- 0.2). Finally, messenger RNA for the neuropeptide Y Y1 receptor was detected using RT-PCR. On the basis of our results we conclude that the neuropeptide Y-induced potentiation of the noradrenaline-induced contraction is mediated by the neuropeptide Y Y1 receptor. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1110302
- author
- Bergdahl, Anders ; Nilsson, Torun ; Cantera, Leonor ; Nilsson, Leif ; Sun, Xiang-Ying ; Hedner, Tomas ; Erlinge, David LU ; Valdemarson, Stig and Edvinsson, Lars LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Artery, human, Noradrenaline, Neuropeptide Y, Antisense oligodeoxynucleotide, BIBP3226, Reverse transcriptase-polymerase chain reaction
- in
- European Journal of Pharmacology
- volume
- 316
- issue
- 1
- pages
- 59 - 64
- publisher
- Elsevier
- external identifiers
-
- pmid:8982651
- scopus:0030605216
- ISSN
- 1879-0712
- DOI
- 10.1016/S0014-2999(96)00636-X
- language
- English
- LU publication?
- yes
- id
- c2c43d90-2ce2-401a-b8a0-a21abb49b4c3 (old id 1110302)
- date added to LUP
- 2016-04-01 12:01:35
- date last changed
- 2024-01-08 05:27:54
@article{c2c43d90-2ce2-401a-b8a0-a21abb49b4c3, abstract = {{To elucidate which neuropeptide Y receptor subtype is responsible for the neuropeptide Y-induced potentiation of the noradrenaline-evoked contraction in human omental arteries we used antisense oligodeoxynucleotide (Antisense), the new selective neuropeptide Y Y1 receptor antagonist, BIBP3226 {(R)-N2-(diphenylacetyl)-N-[(4-hydroxyphenyl) methyl]-D-arginine-amide} and the reverse transcriptase-polymerase chain reaction (RT-PCR). Neuropeptide Y significantly potentiated the noradrenaline-induced contraction in non-incubated vessels (pEC50 6.4 +/- 0.2 vs. 5.9 +/- 0.2) and in vessels incubated with 1 microM Sense oligodeoxynucleotide (Sense) (pEC50 6.0 +/- 0.1 vs. 5.6 +/- 0.2). In vessels incubated with 1 microM Antisense the potentiating effect of neuropeptide Y was completely abolished. BIBP3226 (1 microM) inhibited the neuropeptide Y-induced potentiation in human omental arteries (pEC50 5.8 +/- 0.3 vs. 6.4 +/- 0.2). Finally, messenger RNA for the neuropeptide Y Y1 receptor was detected using RT-PCR. On the basis of our results we conclude that the neuropeptide Y-induced potentiation of the noradrenaline-induced contraction is mediated by the neuropeptide Y Y1 receptor.}}, author = {{Bergdahl, Anders and Nilsson, Torun and Cantera, Leonor and Nilsson, Leif and Sun, Xiang-Ying and Hedner, Tomas and Erlinge, David and Valdemarson, Stig and Edvinsson, Lars}}, issn = {{1879-0712}}, keywords = {{Artery; human; Noradrenaline; Neuropeptide Y; Antisense oligodeoxynucleotide; BIBP3226; Reverse transcriptase-polymerase chain reaction}}, language = {{eng}}, number = {{1}}, pages = {{59--64}}, publisher = {{Elsevier}}, series = {{European Journal of Pharmacology}}, title = {{Neuropeptide Y potentiates noradrenaline-induced contraction through the neuropeptide Y Y1 receptor}}, url = {{http://dx.doi.org/10.1016/S0014-2999(96)00636-X}}, doi = {{10.1016/S0014-2999(96)00636-X}}, volume = {{316}}, year = {{1996}}, }