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Involvement of CCL25 (TECK) in the generation of the murine small-intestinal CD8alpha alpha+CD3+ intraepithelial lymphocyte compartment.

Marsal, Jan LU ; Svensson Frej, Marcus LU ; Ericsson, Anna LU ; Feridani, Amir LU ; Carramolino, Laura; Márquez, Gabriel and Agace, William LU (2002) In European Journal of Immunology 32(12). p.3488-3497
Abstract
The CC chemokine CCL25 (TECK) is selectively expressed in the thymus and small intestine, indicating a potential role in T lymphocyte development. In the present study we examined the role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte (IEL) compartment. CCL25 mRNA expression in the murine small intestine increased at three weeks of age and corresponded with the appearance of CD8alpha alpha(+)CD3(+) lymphocytes in the small-intestinal epithelium. Administration of monoclonal neutralizing anti-CCL25 antibody to two-week-old mice led to a approximately 50% reduction in the total number of CD8alpha alpha(+)TCRgamma delta(+) and CD8alpha alpha(+)TCRalpha beta(+) IEL at four weeks of age.... (More)
The CC chemokine CCL25 (TECK) is selectively expressed in the thymus and small intestine, indicating a potential role in T lymphocyte development. In the present study we examined the role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte (IEL) compartment. CCL25 mRNA expression in the murine small intestine increased at three weeks of age and corresponded with the appearance of CD8alpha alpha(+)CD3(+) lymphocytes in the small-intestinal epithelium. Administration of monoclonal neutralizing anti-CCL25 antibody to two-week-old mice led to a approximately 50% reduction in the total number of CD8alpha alpha(+)TCRgamma delta(+) and CD8alpha alpha(+)TCRalpha beta(+) IEL at four weeks of age. Freshly isolated murine CD8alpha alpha(+)CD3(+) IEL migrated in response to CCL25 and expressed the CCL25 receptor, CCR9. Analysis of CCR9 expression on putative IEL precursor populations demonstrated the presence of both CCR9(-) and CCR9(+) cells and indicated that up-regulation of this receptor occurred during IEL precursor differentiation. Finally, data from wild-type and RAG(-/-) mice suggested that the reduction in CD8alpha alpha(+)CD3(+) IEL in anti-CCL25 antibody treated mice resulted primarily from defective maintenance and/or development of IEL precursors rather than a direct effect on mature CD8alpha alpha(+)CD3(+) IEL. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chemokine, Mucosa, Intraepithelial lymphocyte
in
European Journal of Immunology
volume
32
issue
12
pages
3488 - 3497
publisher
John Wiley & Sons
external identifiers
  • wos:000179907000017
  • pmid:12442331
  • scopus:0036910773
ISSN
1521-4141
DOI
10.1002/1521-4141(200212)32:12<3488::AID-IMMU3488>3.0.CO;2-E
language
English
LU publication?
yes
id
b1e5dcfb-5023-4b3d-ab4f-5d16fecd72a9 (old id 111089)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12442331&dopt=Abstract
date added to LUP
2007-07-17 13:54:41
date last changed
2017-08-27 04:19:25
@article{b1e5dcfb-5023-4b3d-ab4f-5d16fecd72a9,
  abstract     = {The CC chemokine CCL25 (TECK) is selectively expressed in the thymus and small intestine, indicating a potential role in T lymphocyte development. In the present study we examined the role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte (IEL) compartment. CCL25 mRNA expression in the murine small intestine increased at three weeks of age and corresponded with the appearance of CD8alpha alpha(+)CD3(+) lymphocytes in the small-intestinal epithelium. Administration of monoclonal neutralizing anti-CCL25 antibody to two-week-old mice led to a approximately 50% reduction in the total number of CD8alpha alpha(+)TCRgamma delta(+) and CD8alpha alpha(+)TCRalpha beta(+) IEL at four weeks of age. Freshly isolated murine CD8alpha alpha(+)CD3(+) IEL migrated in response to CCL25 and expressed the CCL25 receptor, CCR9. Analysis of CCR9 expression on putative IEL precursor populations demonstrated the presence of both CCR9(-) and CCR9(+) cells and indicated that up-regulation of this receptor occurred during IEL precursor differentiation. Finally, data from wild-type and RAG(-/-) mice suggested that the reduction in CD8alpha alpha(+)CD3(+) IEL in anti-CCL25 antibody treated mice resulted primarily from defective maintenance and/or development of IEL precursors rather than a direct effect on mature CD8alpha alpha(+)CD3(+) IEL.},
  author       = {Marsal, Jan and Svensson Frej, Marcus and Ericsson, Anna and Feridani, Amir and Carramolino, Laura and Márquez, Gabriel and Agace, William},
  issn         = {1521-4141},
  keyword      = {Chemokine,Mucosa,Intraepithelial lymphocyte},
  language     = {eng},
  number       = {12},
  pages        = {3488--3497},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {Involvement of CCL25 (TECK) in the generation of the murine small-intestinal CD8alpha alpha+CD3+ intraepithelial lymphocyte compartment.},
  url          = {http://dx.doi.org/10.1002/1521-4141(200212)32:12<3488::AID-IMMU3488>3.0.CO;2-E},
  volume       = {32},
  year         = {2002},
}