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Decrease in high density lipoprotein cholesterol during prolonged storage. CELL Study Group

Ekbom, Tord LU ; Lindholm, L H; Lanke, Jan LU and Nilsson-Ehle, Peter LU (1996) In Scandinavian Journal of Clinical & Laboratory Investigation 56(2). p.97-101
Abstract
Different studies on the stability of high density lipoprotein cholesterol (HDL) in frozen serum or plasma have yielded conflicting results, namely increase, decrease, or no change at all during prolonged storage under freezing conditions. As part of a major trial on lipid-lowering strategies we statistically demonstrated a time-related decrease in HDL cholesterol during storage up to 46 months at -20 degrees C. We therefore re-analysed 85 frozen samples that had been analysed fresh and then stored from 26 to 46 months, using the dextran sulphate 500/Mg2+ method. A linear regression analysis of change in HDL cholesterol on time was performed. The slope was significantly negative (p < 0.0005). The regression equation was (decrease in... (More)
Different studies on the stability of high density lipoprotein cholesterol (HDL) in frozen serum or plasma have yielded conflicting results, namely increase, decrease, or no change at all during prolonged storage under freezing conditions. As part of a major trial on lipid-lowering strategies we statistically demonstrated a time-related decrease in HDL cholesterol during storage up to 46 months at -20 degrees C. We therefore re-analysed 85 frozen samples that had been analysed fresh and then stored from 26 to 46 months, using the dextran sulphate 500/Mg2+ method. A linear regression analysis of change in HDL cholesterol on time was performed. The slope was significantly negative (p < 0.0005). The regression equation was (decrease in HDL) = 0.05 - 0.008 x (time in months), i.e. after 6 months' storage at -20 degrees C there was almost a 1% decrease in the HDL cholesterol concentration per month of storage. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
analysis, frozen specimen, high density lipoprotein, storage
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
56
issue
2
pages
97 - 101
publisher
Informa Healthcare
external identifiers
  • pmid:8743100
ISSN
1502-7686
DOI
10.3109/00365519609088594
language
English
LU publication?
yes
id
ebf81ffe-6789-45b1-9d24-eb6a8bcf9e67 (old id 1110934)
date added to LUP
2008-07-22 11:26:42
date last changed
2016-04-16 03:55:14
@article{ebf81ffe-6789-45b1-9d24-eb6a8bcf9e67,
  abstract     = {Different studies on the stability of high density lipoprotein cholesterol (HDL) in frozen serum or plasma have yielded conflicting results, namely increase, decrease, or no change at all during prolonged storage under freezing conditions. As part of a major trial on lipid-lowering strategies we statistically demonstrated a time-related decrease in HDL cholesterol during storage up to 46 months at -20 degrees C. We therefore re-analysed 85 frozen samples that had been analysed fresh and then stored from 26 to 46 months, using the dextran sulphate 500/Mg2+ method. A linear regression analysis of change in HDL cholesterol on time was performed. The slope was significantly negative (p &lt; 0.0005). The regression equation was (decrease in HDL) = 0.05 - 0.008 x (time in months), i.e. after 6 months' storage at -20 degrees C there was almost a 1% decrease in the HDL cholesterol concentration per month of storage.},
  author       = {Ekbom, Tord and Lindholm, L H and Lanke, Jan and Nilsson-Ehle, Peter},
  issn         = {1502-7686},
  keyword      = {analysis,frozen specimen,high density lipoprotein,storage},
  language     = {eng},
  number       = {2},
  pages        = {97--101},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Decrease in high density lipoprotein cholesterol during prolonged storage. CELL Study Group},
  url          = {http://dx.doi.org/10.3109/00365519609088594},
  volume       = {56},
  year         = {1996},
}