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The effect of endothelin receptor blockade on the development of the Sephadex-induced inflammation in the rat lung

Andersson, Sven LU ; Hemsen, A and Lundberg, J M (1996) In Acta Physiologica Scandinavica 158(2). p.189-193
Abstract
The non-peptide ET-receptor antagonist Bosentan was used to investigate the role of endogenous endothelin-1 (ET-1) in the development of the Sephadex-induced lung inflammation in the rat. Intratracheal instillation of Sephadex caused a 60-fold rise in endothelin-1-like-immunoreactivity (ET-1-LI) in bronchoalveolar lavage fluid (BALF) concomitant with development of lung oedema, an influx of inflammatory cells into the airways and a rise in the protein content in BALF. The ET-1-LI level in lung homogenate was not significantly affected. Pre-treatment with Bosentan reduced ET-1-LI content in the lung parenchyma but increased ET-1-LI levels in BALF, possibly indicating an effective displacement of ET-1 from its receptors. In Bosentan-treated... (More)
The non-peptide ET-receptor antagonist Bosentan was used to investigate the role of endogenous endothelin-1 (ET-1) in the development of the Sephadex-induced lung inflammation in the rat. Intratracheal instillation of Sephadex caused a 60-fold rise in endothelin-1-like-immunoreactivity (ET-1-LI) in bronchoalveolar lavage fluid (BALF) concomitant with development of lung oedema, an influx of inflammatory cells into the airways and a rise in the protein content in BALF. The ET-1-LI level in lung homogenate was not significantly affected. Pre-treatment with Bosentan reduced ET-1-LI content in the lung parenchyma but increased ET-1-LI levels in BALF, possibly indicating an effective displacement of ET-1 from its receptors. In Bosentan-treated animals there was an enhancement of the lung oedema formation following Sephadex instillation, but no significant change in the number of leucocytes or protein concentration in BALF. The present data thus do not support the hypothesis that endogenous ET-1 mediates oedema formation or leucocyte influx in this model. If anything, Bosentan enhanced the oedema formation in parallel with increased ET-1-LI in BALF. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bronchoalveolar lavage, Bosentan, endothelin, leucocyte recruitment, oedema, rat lung, receptor antagonism, Sephadex
in
Acta Physiologica Scandinavica
volume
158
issue
2
pages
189 - 193
publisher
Wiley-Blackwell
external identifiers
  • pmid:8899066
  • scopus:0029819837
ISSN
0001-6772
DOI
10.1046/j.1365-201X.1996.543303000.x
language
English
LU publication?
yes
id
6b23956a-7b03-4e50-a4c0-6ed38f32edc7 (old id 1111105)
date added to LUP
2008-07-21 10:12:27
date last changed
2017-01-01 07:08:51
@article{6b23956a-7b03-4e50-a4c0-6ed38f32edc7,
  abstract     = {The non-peptide ET-receptor antagonist Bosentan was used to investigate the role of endogenous endothelin-1 (ET-1) in the development of the Sephadex-induced lung inflammation in the rat. Intratracheal instillation of Sephadex caused a 60-fold rise in endothelin-1-like-immunoreactivity (ET-1-LI) in bronchoalveolar lavage fluid (BALF) concomitant with development of lung oedema, an influx of inflammatory cells into the airways and a rise in the protein content in BALF. The ET-1-LI level in lung homogenate was not significantly affected. Pre-treatment with Bosentan reduced ET-1-LI content in the lung parenchyma but increased ET-1-LI levels in BALF, possibly indicating an effective displacement of ET-1 from its receptors. In Bosentan-treated animals there was an enhancement of the lung oedema formation following Sephadex instillation, but no significant change in the number of leucocytes or protein concentration in BALF. The present data thus do not support the hypothesis that endogenous ET-1 mediates oedema formation or leucocyte influx in this model. If anything, Bosentan enhanced the oedema formation in parallel with increased ET-1-LI in BALF.},
  author       = {Andersson, Sven and Hemsen, A and Lundberg, J M},
  issn         = {0001-6772},
  keyword      = {Bronchoalveolar lavage,Bosentan,endothelin,leucocyte recruitment,oedema,rat lung,receptor antagonism,Sephadex},
  language     = {eng},
  number       = {2},
  pages        = {189--193},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Physiologica Scandinavica},
  title        = {The effect of endothelin receptor blockade on the development of the Sephadex-induced inflammation in the rat lung},
  url          = {http://dx.doi.org/10.1046/j.1365-201X.1996.543303000.x},
  volume       = {158},
  year         = {1996},
}