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Release of endothelin-1 into rat airways following Sephadex-induced inflammation; modulation by enzyme inhibitors and budesonide

Andersson, Sven LU ; Hemsen, A; Zackrisson, C and Lundberg, J M (1996) In Respiration 63(2). p.111-116
Abstract
The intratracheal (i.t.) instillation of Sephadex beads into rat induced inflammation and a 30-fold increase in the endothelin-1-like immunoreactivity (ET-1-LI) of broncho-alveolar lavage fluid. The levels were highest 24 h after the instillation and had declined significantly after 48 h. At a dose of 1 mg kg-1 i.t., the glucocorticosteroid budesonide almost abolished this response. Phosphoramidon, which inhibits neutral endopeptidase, an enzyme reported to degrade ET-1 and also to inhibit the endothelin-converting enzyme, potentiated the Sephadex-induced rise in ET-1-LI. Chymostatin and heparin, which are reported to reduce the formation of ET-1, did not affect the increase in ET-1-LI. The present model represents a very reactive system... (More)
The intratracheal (i.t.) instillation of Sephadex beads into rat induced inflammation and a 30-fold increase in the endothelin-1-like immunoreactivity (ET-1-LI) of broncho-alveolar lavage fluid. The levels were highest 24 h after the instillation and had declined significantly after 48 h. At a dose of 1 mg kg-1 i.t., the glucocorticosteroid budesonide almost abolished this response. Phosphoramidon, which inhibits neutral endopeptidase, an enzyme reported to degrade ET-1 and also to inhibit the endothelin-converting enzyme, potentiated the Sephadex-induced rise in ET-1-LI. Chymostatin and heparin, which are reported to reduce the formation of ET-1, did not affect the increase in ET-1-LI. The present model represents a very reactive system for analyzing the changes in ET-1 levels during inflammation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Respiration
volume
63
issue
2
pages
111 - 116
publisher
Karger
external identifiers
  • pmid:8966364
  • scopus:0029931775
ISSN
1423-0356
language
English
LU publication?
yes
id
2b2e5f37-0f36-4ef3-b1d6-30c57eafb13c (old id 1111108)
date added to LUP
2008-07-21 10:07:25
date last changed
2017-01-01 07:12:23
@article{2b2e5f37-0f36-4ef3-b1d6-30c57eafb13c,
  abstract     = {The intratracheal (i.t.) instillation of Sephadex beads into rat induced inflammation and a 30-fold increase in the endothelin-1-like immunoreactivity (ET-1-LI) of broncho-alveolar lavage fluid. The levels were highest 24 h after the instillation and had declined significantly after 48 h. At a dose of 1 mg kg-1 i.t., the glucocorticosteroid budesonide almost abolished this response. Phosphoramidon, which inhibits neutral endopeptidase, an enzyme reported to degrade ET-1 and also to inhibit the endothelin-converting enzyme, potentiated the Sephadex-induced rise in ET-1-LI. Chymostatin and heparin, which are reported to reduce the formation of ET-1, did not affect the increase in ET-1-LI. The present model represents a very reactive system for analyzing the changes in ET-1 levels during inflammation.},
  author       = {Andersson, Sven and Hemsen, A and Zackrisson, C and Lundberg, J M},
  issn         = {1423-0356},
  language     = {eng},
  number       = {2},
  pages        = {111--116},
  publisher    = {Karger},
  series       = {Respiration},
  title        = {Release of endothelin-1 into rat airways following Sephadex-induced inflammation; modulation by enzyme inhibitors and budesonide},
  volume       = {63},
  year         = {1996},
}