Advanced

Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells

Ding, W Q; Larsson, Christer LU ; Simonsson, Per LU and Alling, Christer LU (1997) In Brain Research. Molecular Brain Research 46(1-2). p.77-84
Abstract
The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol... (More)
The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muscarinic acetylcholine receptor, Ethanol, c-fos expression, Protein kinase C, Neuroblastoma SH-SY5Y cell
in
Brain Research. Molecular Brain Research
volume
46
issue
1-2
pages
77 - 84
publisher
Elsevier
external identifiers
  • pmid:9191080
  • scopus:0030911415
ISSN
0169-328X
DOI
10.1016/S0169-328X(96)00273-2
language
English
LU publication?
yes
id
962d2d5d-8b3d-4304-a616-596ad4d14622 (old id 1111179)
date added to LUP
2008-07-16 16:52:32
date last changed
2017-01-01 06:53:26
@article{962d2d5d-8b3d-4304-a616-596ad4d14622,
  abstract     = {The effect of ethanol exposure on muscarinic receptor-stimulated expression of c-fos was investigated in SH-SY5Y cells. Four days of ethanol exposure enhanced carbachol-stimulated c-fos mRNA expression, analyzed with Northern blot, and Fos/AP-1 binding activity, measured with gel mobility super shift assay. Pre-incubation with muscarinic antagonists or the protein kinase C inhibitor GF109203X demonstrated that, in both control and ethanol-treated cells, carbachol-induced c-fos expression was mediated via muscarinic M1 receptors and to a large extent through protein kinase C. However, phorbol ester-induced c-fos expression was unaffected in ethanol-treated cells. Acute exposure to ethanol caused a suppression of both carbachol- and phorbol ester-stimulated c-fos expression. These results demonstrate that muscarinic receptor-stimulated gene expression is sensitive to both acute and long-term ethanol exposure.},
  author       = {Ding, W Q and Larsson, Christer and Simonsson, Per and Alling, Christer},
  issn         = {0169-328X},
  keyword      = {Muscarinic acetylcholine receptor,Ethanol,c-fos expression,Protein kinase C,Neuroblastoma SH-SY5Y cell},
  language     = {eng},
  number       = {1-2},
  pages        = {77--84},
  publisher    = {Elsevier},
  series       = {Brain Research. Molecular Brain Research},
  title        = {Effects of ethanol on muscarinic receptor-stimulated c-fos expression in human neuroblastoma cells},
  url          = {http://dx.doi.org/10.1016/S0169-328X(96)00273-2},
  volume       = {46},
  year         = {1997},
}