Partial purification and identification of hormone-sensitive lipase from chicken adipose tissue
(1997) In Biochemical and Biophysical Research Communications 236(1). p.94-99- Abstract
- HSL from chicken adipose tissue exhibits remarkable activation upon phosphorylation with cAMP-dependent protein kinase (cAMP-PK) compared to HSL from rat and human adipose tissue. In order to characterize the chicken HSL enzyme, it was purified 3500 fold from a chicken adipose tissue homogenate using pH 5.2 precipitation and anion-exchange chromatography. The purified chicken HSL was identified as an 86 kDa protein using Western blot analysis. The HSL diacylglycerol lipase activity was inhibited by 98% upon incubation with anti-rat HSL antiserum, and the specific activity of chicken HSL was estimated to be approximately the same as for the rat enzyme. Furthermore, the 86 kDa polypeptide was phosphorylated by cAMP-PK to about the same... (More)
- HSL from chicken adipose tissue exhibits remarkable activation upon phosphorylation with cAMP-dependent protein kinase (cAMP-PK) compared to HSL from rat and human adipose tissue. In order to characterize the chicken HSL enzyme, it was purified 3500 fold from a chicken adipose tissue homogenate using pH 5.2 precipitation and anion-exchange chromatography. The purified chicken HSL was identified as an 86 kDa protein using Western blot analysis. The HSL diacylglycerol lipase activity was inhibited by 98% upon incubation with anti-rat HSL antiserum, and the specific activity of chicken HSL was estimated to be approximately the same as for the rat enzyme. Furthermore, the 86 kDa polypeptide was phosphorylated by cAMP-PK to about the same stoichiometry as for the recombinant rat enzyme. Hence, our results demonstrate that HSL from chicken adipose tissue is comparable in size and specific activity to HSL from mammalian species, and not a smaller 42 kDa polypeptide with 1000-fold lower specific activity as previously reported. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111255
- author
- Anthonsen, Marit W ; Degerman, Eva LU and Holm, Cecilia LU
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochemical and Biophysical Research Communications
- volume
- 236
- issue
- 1
- pages
- 94 - 99
- publisher
- Elsevier
- external identifiers
-
- pmid:9223433
- scopus:0031561103
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.1997.6923
- language
- English
- LU publication?
- yes
- id
- b010ed73-cca9-468a-9129-b7eb2be49a25 (old id 1111255)
- date added to LUP
- 2016-04-01 16:23:41
- date last changed
- 2022-05-27 19:30:53
@article{b010ed73-cca9-468a-9129-b7eb2be49a25, abstract = {{HSL from chicken adipose tissue exhibits remarkable activation upon phosphorylation with cAMP-dependent protein kinase (cAMP-PK) compared to HSL from rat and human adipose tissue. In order to characterize the chicken HSL enzyme, it was purified 3500 fold from a chicken adipose tissue homogenate using pH 5.2 precipitation and anion-exchange chromatography. The purified chicken HSL was identified as an 86 kDa protein using Western blot analysis. The HSL diacylglycerol lipase activity was inhibited by 98% upon incubation with anti-rat HSL antiserum, and the specific activity of chicken HSL was estimated to be approximately the same as for the rat enzyme. Furthermore, the 86 kDa polypeptide was phosphorylated by cAMP-PK to about the same stoichiometry as for the recombinant rat enzyme. Hence, our results demonstrate that HSL from chicken adipose tissue is comparable in size and specific activity to HSL from mammalian species, and not a smaller 42 kDa polypeptide with 1000-fold lower specific activity as previously reported.}}, author = {{Anthonsen, Marit W and Degerman, Eva and Holm, Cecilia}}, issn = {{1090-2104}}, language = {{eng}}, number = {{1}}, pages = {{94--99}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Partial purification and identification of hormone-sensitive lipase from chicken adipose tissue}}, url = {{http://dx.doi.org/10.1006/bbrc.1997.6923}}, doi = {{10.1006/bbrc.1997.6923}}, volume = {{236}}, year = {{1997}}, }