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Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients

Gustafson, Pelle LU ; Fernö, Mårten LU ; Åkerman, Måns LU ; Baldetorp, Bo LU ; Willen, H; Killander, Dick LU and Rydholm, Anders LU (1997) In British Journal of Cancer 75(1). p.94-100
Abstract
We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (6-31) years. In a univariate analysis, deep tumour location, increasing tumour size and histological malignancy grade, microscopic tumour necrosis, vascular invasion, DNA non-diploidy and high SPF (>3.0%) were associated with poor metastasis-free survival. In a multivariate analysis, microscopic tumour necrosis and high SPF were independently prognostic for metastasis. Used in combination with tumour size, microscopic tumour necrosis and... (More)
We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (6-31) years. In a univariate analysis, deep tumour location, increasing tumour size and histological malignancy grade, microscopic tumour necrosis, vascular invasion, DNA non-diploidy and high SPF (>3.0%) were associated with poor metastasis-free survival. In a multivariate analysis, microscopic tumour necrosis and high SPF were independently prognostic for metastasis. Used in combination with tumour size, microscopic tumour necrosis and vascular invasion, SPF could identify a group of patients with a 5-year metastasis-free survival rate of 0.97. This group constituted one-quarter of all patients. Patients with low SPF who did recur had a prolonged clinical course both as regards metastases and local recurrence. We conclude that SPF is a valuable adjunct in prognostication in soft-tissue sarcoma. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Soft tissue, Sarcoma, S Phase, Flow cytometry, Cell cycle, DNA, Ploidy, Prognosis, Human, Malignant tumor
in
British Journal of Cancer
volume
75
issue
1
pages
94 - 100
publisher
Nature Publishing Group
external identifiers
  • pmid:9000604
  • scopus:0031012346
ISSN
1532-1827
language
English
LU publication?
yes
id
4d7ac175-e16b-4be4-8a6e-ec0c4795b635 (old id 1111408)
date added to LUP
2008-07-17 12:16:31
date last changed
2017-01-01 05:14:26
@article{4d7ac175-e16b-4be4-8a6e-ec0c4795b635,
  abstract     = {We could determine the S-phase fraction (SPF) by flow cytometric DNA analysis of paraffin archival material in 160 of 260 patients with soft-tissue sarcoma of extremity and trunk wall. The prognostic value of SPF was compared with other clinicopathological factors. The median follow-up time was 16 (6-31) years. In a univariate analysis, deep tumour location, increasing tumour size and histological malignancy grade, microscopic tumour necrosis, vascular invasion, DNA non-diploidy and high SPF (>3.0%) were associated with poor metastasis-free survival. In a multivariate analysis, microscopic tumour necrosis and high SPF were independently prognostic for metastasis. Used in combination with tumour size, microscopic tumour necrosis and vascular invasion, SPF could identify a group of patients with a 5-year metastasis-free survival rate of 0.97. This group constituted one-quarter of all patients. Patients with low SPF who did recur had a prolonged clinical course both as regards metastases and local recurrence. We conclude that SPF is a valuable adjunct in prognostication in soft-tissue sarcoma.},
  author       = {Gustafson, Pelle and Fernö, Mårten and Åkerman, Måns and Baldetorp, Bo and Willen, H and Killander, Dick and Rydholm, Anders},
  issn         = {1532-1827},
  keyword      = {Soft tissue,Sarcoma,S Phase,Flow cytometry,Cell cycle,DNA,Ploidy,Prognosis,Human,Malignant tumor},
  language     = {eng},
  number       = {1},
  pages        = {94--100},
  publisher    = {Nature Publishing Group},
  series       = {British Journal of Cancer},
  title        = {Flow cytometric S-phase fraction in soft-tissue sarcoma: prognostic importance analysed in 160 patients},
  volume       = {75},
  year         = {1997},
}