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S-100beta stimulates neurite outgrowth in the rat sciatic nerve grafted with acellular muscle transplants

Haglid, Kenneth G; Yang, Qiner; Hamberger, Anders; Bergman, Stina; Widerberg, Annika LU and Danielsen, Nils LU (1997) In Brain Research 753(2). p.196-201
Abstract
S-100beta promotes neurite extension in vitro and motoneuron survival in the chicken embryo. We demonstrate here that local administration of S-100beta stimulates the sciatic nerve regeneration into acellular muscle grafts. Normally there is a 8-10 day delay in the regeneration of axons into such grafts. Local administration of S-100beta (0.5-1.0 microg/h) significantly stimulated regeneration into the grafts. In S-100beta treated grafts, the regeneration distance was increased with a factor of about 2.3 times as compared to vehicle treated grafts. The distance of regeneration was monitored with pinch test which detects sensory axons. Regenerating axons were growing outside the necrotic muscle cells as revealed with immunohistochemistry... (More)
S-100beta promotes neurite extension in vitro and motoneuron survival in the chicken embryo. We demonstrate here that local administration of S-100beta stimulates the sciatic nerve regeneration into acellular muscle grafts. Normally there is a 8-10 day delay in the regeneration of axons into such grafts. Local administration of S-100beta (0.5-1.0 microg/h) significantly stimulated regeneration into the grafts. In S-100beta treated grafts, the regeneration distance was increased with a factor of about 2.3 times as compared to vehicle treated grafts. The distance of regeneration was monitored with pinch test which detects sensory axons. Regenerating axons were growing outside the necrotic muscle cells as revealed with immunohistochemistry for the neurofilament light weight polypeptide. S-100beta was demonstrated immunocytochemically in motor neurons of the rat lumbar spinal cord and in large and medium sized neurons of the dorsal root ganglia. The results suggest that S-100beta is a physiological growth factor for peripheral nerve axons. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Axotomy, Nerve regeneration, Neurotrophic effect, Sensory test
in
Brain Research
volume
753
issue
2
pages
196 - 201
publisher
Elsevier
external identifiers
  • pmid:9125403
  • scopus:0031564528
ISSN
1872-6240
DOI
10.1016/S0006-8993(96)01463-1
language
English
LU publication?
yes
id
e46bfeed-749c-41d7-91ba-a0454f2141ac (old id 1111979)
date added to LUP
2008-07-21 11:07:32
date last changed
2017-02-26 03:36:24
@article{e46bfeed-749c-41d7-91ba-a0454f2141ac,
  abstract     = {S-100beta promotes neurite extension in vitro and motoneuron survival in the chicken embryo. We demonstrate here that local administration of S-100beta stimulates the sciatic nerve regeneration into acellular muscle grafts. Normally there is a 8-10 day delay in the regeneration of axons into such grafts. Local administration of S-100beta (0.5-1.0 microg/h) significantly stimulated regeneration into the grafts. In S-100beta treated grafts, the regeneration distance was increased with a factor of about 2.3 times as compared to vehicle treated grafts. The distance of regeneration was monitored with pinch test which detects sensory axons. Regenerating axons were growing outside the necrotic muscle cells as revealed with immunohistochemistry for the neurofilament light weight polypeptide. S-100beta was demonstrated immunocytochemically in motor neurons of the rat lumbar spinal cord and in large and medium sized neurons of the dorsal root ganglia. The results suggest that S-100beta is a physiological growth factor for peripheral nerve axons.},
  author       = {Haglid, Kenneth G and Yang, Qiner and Hamberger, Anders and Bergman, Stina and Widerberg, Annika and Danielsen, Nils},
  issn         = {1872-6240},
  keyword      = {Axotomy,Nerve regeneration,Neurotrophic effect,Sensory test},
  language     = {eng},
  number       = {2},
  pages        = {196--201},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {S-100beta stimulates neurite outgrowth in the rat sciatic nerve grafted with acellular muscle transplants},
  url          = {http://dx.doi.org/10.1016/S0006-8993(96)01463-1},
  volume       = {753},
  year         = {1997},
}