Advanced

Monitoring of polychlorinated biphenyls in human blood plasma: methodological developments and influence of age, lactation, and fish consumption

Grimvall, E; Rylander, Lars LU ; Nilsson-Ehle, Peter LU ; Nilsson, U; Strömberg, Ulf LU ; Hagmar, L and Östman, C (1997) In Archives of Environmental Contamination and Toxicology 32(3). p.329-336
Abstract
Human plasma samples from 50 wives of fishermen have been analyzed with respect to PCBs. The non-ortho-substituted PCB congeners CB-126 and CB-169 were determined by mass spectrometry in negative ion chemical ionization mode, which demonstrated a limit of detection of 30 fg. The recoveries of the internal standards used for determination of ortho-substituted CBs were approximately 95%. Two methods, one gravimetric and the other based on enzymatic determinations of triglycerides, cholesterol and phospholipids, were compared for the determination of total amount of lipids in the plasma samples; the correlation coefficient was 0.82 and the slope 0.98. For practical reasons, enzymatic determinations are recommended for further use. The total,... (More)
Human plasma samples from 50 wives of fishermen have been analyzed with respect to PCBs. The non-ortho-substituted PCB congeners CB-126 and CB-169 were determined by mass spectrometry in negative ion chemical ionization mode, which demonstrated a limit of detection of 30 fg. The recoveries of the internal standards used for determination of ortho-substituted CBs were approximately 95%. Two methods, one gravimetric and the other based on enzymatic determinations of triglycerides, cholesterol and phospholipids, were compared for the determination of total amount of lipids in the plasma samples; the correlation coefficient was 0.82 and the slope 0.98. For practical reasons, enzymatic determinations are recommended for further use. The total, lipid-adjusted concentrations of PCBs in plasma were influenced by age, total lactation time and consumption of fatty fish from the Baltic Sea. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Archives of Environmental Contamination and Toxicology
volume
32
issue
3
pages
329 - 336
publisher
Springer
external identifiers
  • pmid:9096084
  • scopus:0030932165
ISSN
0090-4341
DOI
10.1007/s002449900193
language
English
LU publication?
yes
id
cc118327-582d-40ad-a517-a1f26f8b70f7 (old id 1112298)
date added to LUP
2008-07-22 09:06:44
date last changed
2017-08-06 03:33:33
@article{cc118327-582d-40ad-a517-a1f26f8b70f7,
  abstract     = {Human plasma samples from 50 wives of fishermen have been analyzed with respect to PCBs. The non-ortho-substituted PCB congeners CB-126 and CB-169 were determined by mass spectrometry in negative ion chemical ionization mode, which demonstrated a limit of detection of 30 fg. The recoveries of the internal standards used for determination of ortho-substituted CBs were approximately 95%. Two methods, one gravimetric and the other based on enzymatic determinations of triglycerides, cholesterol and phospholipids, were compared for the determination of total amount of lipids in the plasma samples; the correlation coefficient was 0.82 and the slope 0.98. For practical reasons, enzymatic determinations are recommended for further use. The total, lipid-adjusted concentrations of PCBs in plasma were influenced by age, total lactation time and consumption of fatty fish from the Baltic Sea.},
  author       = {Grimvall, E and Rylander, Lars and Nilsson-Ehle, Peter and Nilsson, U and Strömberg, Ulf and Hagmar, L and Östman, C},
  issn         = {0090-4341},
  language     = {eng},
  number       = {3},
  pages        = {329--336},
  publisher    = {Springer},
  series       = {Archives of Environmental Contamination and Toxicology},
  title        = {Monitoring of polychlorinated biphenyls in human blood plasma: methodological developments and influence of age, lactation, and fish consumption},
  url          = {http://dx.doi.org/10.1007/s002449900193},
  volume       = {32},
  year         = {1997},
}