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Addition of calcium to Euro-Collins solution is essential for 24-hour preservation of the vasculature

Ingemansson, Richard LU ; Bolys, Ramunas; Budrikis, Algimantas; Lindgren, Anna; Sjöberg, Trygve LU and Steen, Stig LU (1997) In Annals of Thoracic Surgery 63(2). p.408-413
Abstract
BACKGROUND: Genuine Euro-Collins solution is calcium free. The aim of this study was to investigate whether the addition of calcium would improve its capacity to preserve the vasculature. METHODS: The infrarenal aorta of Sprague-Dawley rats was investigated in organ baths: as fresh controls, after 24 hours of cold (4 degrees C) storage in Euro-Collins solution, or in Euro-Collins solution with the addition of calcium in amounts ranging from 0.05 to 1.5 mmol/L. The thromboxane analogue U-46619 was used to investigate contractility. Endothelium-dependent relaxation was tested by cumulative addition of acetylcholine. Papaverine was used to elicit endothelium-independent relaxation. Investigation by transmission electron microscopy was also... (More)
BACKGROUND: Genuine Euro-Collins solution is calcium free. The aim of this study was to investigate whether the addition of calcium would improve its capacity to preserve the vasculature. METHODS: The infrarenal aorta of Sprague-Dawley rats was investigated in organ baths: as fresh controls, after 24 hours of cold (4 degrees C) storage in Euro-Collins solution, or in Euro-Collins solution with the addition of calcium in amounts ranging from 0.05 to 1.5 mmol/L. The thromboxane analogue U-46619 was used to investigate contractility. Endothelium-dependent relaxation was tested by cumulative addition of acetylcholine. Papaverine was used to elicit endothelium-independent relaxation. Investigation by transmission electron microscopy was also performed. RESULTS: Storage of rat aorta for 24 hours in genuine Euro-Collins solution almost abolished smooth muscle function, and severe edema was found in the endothelial cells. However, if calcium was added, the rat aorta could be stored for 24 hours without affecting smooth muscle function, and endothelium-dependent relaxation was only slightly reduced. Furthermore, only slight edema could be demonstrated in the endothelial cells. CONCLUSIONS: If calcium is added to Euro-Collins solution in amounts ranging from 0.4 to 1.5 mmol/L, it allows good preservation of rat aorta for 24 hours. Without calcium, this solution destroys both the function and morphology of the vessels. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Annals of Thoracic Surgery
volume
63
issue
2
pages
408 - 413
publisher
Elsevier
external identifiers
  • pmid:9033310
  • scopus:0031045710
ISSN
1552-6259
language
English
LU publication?
yes
id
c99a1c12-89ac-4d8a-a696-959485d3cdd3 (old id 1112377)
alternative location
http://ats.ctsnetjournals.org/cgi/content/full/63/2/408
date added to LUP
2008-07-22 10:24:00
date last changed
2017-01-01 05:18:40
@article{c99a1c12-89ac-4d8a-a696-959485d3cdd3,
  abstract     = {BACKGROUND: Genuine Euro-Collins solution is calcium free. The aim of this study was to investigate whether the addition of calcium would improve its capacity to preserve the vasculature. METHODS: The infrarenal aorta of Sprague-Dawley rats was investigated in organ baths: as fresh controls, after 24 hours of cold (4 degrees C) storage in Euro-Collins solution, or in Euro-Collins solution with the addition of calcium in amounts ranging from 0.05 to 1.5 mmol/L. The thromboxane analogue U-46619 was used to investigate contractility. Endothelium-dependent relaxation was tested by cumulative addition of acetylcholine. Papaverine was used to elicit endothelium-independent relaxation. Investigation by transmission electron microscopy was also performed. RESULTS: Storage of rat aorta for 24 hours in genuine Euro-Collins solution almost abolished smooth muscle function, and severe edema was found in the endothelial cells. However, if calcium was added, the rat aorta could be stored for 24 hours without affecting smooth muscle function, and endothelium-dependent relaxation was only slightly reduced. Furthermore, only slight edema could be demonstrated in the endothelial cells. CONCLUSIONS: If calcium is added to Euro-Collins solution in amounts ranging from 0.4 to 1.5 mmol/L, it allows good preservation of rat aorta for 24 hours. Without calcium, this solution destroys both the function and morphology of the vessels.},
  author       = {Ingemansson, Richard and Bolys, Ramunas and Budrikis, Algimantas and Lindgren, Anna and Sjöberg, Trygve and Steen, Stig},
  issn         = {1552-6259},
  language     = {eng},
  number       = {2},
  pages        = {408--413},
  publisher    = {Elsevier},
  series       = {Annals of Thoracic Surgery},
  title        = {Addition of calcium to Euro-Collins solution is essential for 24-hour preservation of the vasculature},
  volume       = {63},
  year         = {1997},
}