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Acute ethanol exposure attenuates expression of junD in human neuroblastoma cells

Ding, W Q; Fried, Ulrik LU ; Larsson, Christer LU and Alling, Christer LU (1998) In Neurochemistry International 33(6). p.525-530
Abstract
This study describes the effects of acute ethanol exposure on the mRNA levels of c-jun,junB and junD in the human neuroblastoma SH-SY5Y cell line. An acute exposure to 100 mM ethanol did not influence the basal and phorbol ester-induced expression of c-jun and junB, whereas the basal mRNA level of junD was attenuated by 30%. This effect was dose- and time-dependent with maximal inhibition being detected 2 h after 100 mM ethanol treatment and the mRNA levels gradually returned towards normal afterwards. Ethanol also inhibited phorbol ester-induced expression of junD. The fact that ethanol did not influence degradation of the junD mRNA suggests that acute ethanol suppresses the transcription of the gene. These results indicate that acute... (More)
This study describes the effects of acute ethanol exposure on the mRNA levels of c-jun,junB and junD in the human neuroblastoma SH-SY5Y cell line. An acute exposure to 100 mM ethanol did not influence the basal and phorbol ester-induced expression of c-jun and junB, whereas the basal mRNA level of junD was attenuated by 30%. This effect was dose- and time-dependent with maximal inhibition being detected 2 h after 100 mM ethanol treatment and the mRNA levels gradually returned towards normal afterwards. Ethanol also inhibited phorbol ester-induced expression of junD. The fact that ethanol did not influence degradation of the junD mRNA suggests that acute ethanol suppresses the transcription of the gene. These results indicate that acute ethanol exerts different effects on expression of Jun transcription factors, suggesting that as compared to c-jun and junB, the junD gene may be more sensitive to acute ethanol treatment in neuronal cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ethanol, jun gene family, SH-SY5Y, AP-1
in
Neurochemistry International
volume
33
issue
6
pages
525 - 530
publisher
Elsevier
external identifiers
  • pmid:10098722
  • scopus:0032465934
ISSN
0197-0186
DOI
10.1016/S0197-0186(98)00060-6
language
English
LU publication?
yes
id
71f23ecb-54ea-4a1f-a5d9-293efa60cccb (old id 1112623)
date added to LUP
2008-07-10 10:36:15
date last changed
2017-01-01 04:54:23
@article{71f23ecb-54ea-4a1f-a5d9-293efa60cccb,
  abstract     = {This study describes the effects of acute ethanol exposure on the mRNA levels of c-jun,junB and junD in the human neuroblastoma SH-SY5Y cell line. An acute exposure to 100 mM ethanol did not influence the basal and phorbol ester-induced expression of c-jun and junB, whereas the basal mRNA level of junD was attenuated by 30%. This effect was dose- and time-dependent with maximal inhibition being detected 2 h after 100 mM ethanol treatment and the mRNA levels gradually returned towards normal afterwards. Ethanol also inhibited phorbol ester-induced expression of junD. The fact that ethanol did not influence degradation of the junD mRNA suggests that acute ethanol suppresses the transcription of the gene. These results indicate that acute ethanol exerts different effects on expression of Jun transcription factors, suggesting that as compared to c-jun and junB, the junD gene may be more sensitive to acute ethanol treatment in neuronal cells.},
  author       = {Ding, W Q and Fried, Ulrik and Larsson, Christer and Alling, Christer},
  issn         = {0197-0186},
  keyword      = {Ethanol,jun gene family,SH-SY5Y,AP-1},
  language     = {eng},
  number       = {6},
  pages        = {525--530},
  publisher    = {Elsevier},
  series       = {Neurochemistry International},
  title        = {Acute ethanol exposure attenuates expression of junD in human neuroblastoma cells},
  url          = {http://dx.doi.org/10.1016/S0197-0186(98)00060-6},
  volume       = {33},
  year         = {1998},
}