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Stimulation of muscarinic receptors induces expression of individual fos and jun genes through different transduction pathways

Ding, Wei-Qun; Larsson, Christer LU and Alling, Christer LU (1998) In Journal of Neurochemistry 70(4). p.1722-1729
Abstract
The transduction pathways coupling muscarinic receptors to induction of fos and jun genes were investigated in neuroblastoma SH-SY5Y cells. Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. This effect was abolished by pretreatment with atropine, indicating an involvement of muscarinic receptors. These genes were also induced by activation of protein kinase C with phorbol ester or by elevating the intracellular Ca2+ concentration with a Ca2+ ionophore. The Ca2+ effect was inhibited by KN-62, suggesting an induction through Ca2+/calmodulin-dependent kinase II. Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately... (More)
The transduction pathways coupling muscarinic receptors to induction of fos and jun genes were investigated in neuroblastoma SH-SY5Y cells. Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. This effect was abolished by pretreatment with atropine, indicating an involvement of muscarinic receptors. These genes were also induced by activation of protein kinase C with phorbol ester or by elevating the intracellular Ca2+ concentration with a Ca2+ ionophore. The Ca2+ effect was inhibited by KN-62, suggesting an induction through Ca2+/calmodulin-dependent kinase II. Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. On the other hand, preincubation with KN-62 attenuated the carbachol-induced increase in c-jun and junD expression by 70% but had no effect on c-fos, fosB, and junB mRNA levels. Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Comparison of the inhibitory effects of GF109203X and Go 6976 suggests the involvement of classical protein kinase C isozymes in muscarinic receptor-stimulated expression of fos and jun genes. These results demonstrate that the muscarinic receptor-induced expression of individual fos and jun genes is regulated via different pathways, primarily protein kinase C or Ca2+/calmodulin-dependent kinase II. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Muscarinic receptor, Protein kinase C, Immediate-early genes, SH-SY5Y neuroblastoma cells, Ca2+/calmodulin-dependent kinase II
in
Journal of Neurochemistry
volume
70
issue
4
pages
1722 - 1729
publisher
Wiley-Blackwell
external identifiers
  • pmid:9523591
  • scopus:0031881692
ISSN
1471-4159
DOI
10.1046/j.1471-4159.1998.70041722.x
language
English
LU publication?
yes
id
daf2abd2-2c23-4560-a0bf-93275d780437 (old id 1112630)
date added to LUP
2008-07-10 10:45:50
date last changed
2017-01-01 07:01:59
@article{daf2abd2-2c23-4560-a0bf-93275d780437,
  abstract     = {The transduction pathways coupling muscarinic receptors to induction of fos and jun genes were investigated in neuroblastoma SH-SY5Y cells. Stimulation with carbachol induced expression of c-fos, fosB, c-jun, junB, and junD. This effect was abolished by pretreatment with atropine, indicating an involvement of muscarinic receptors. These genes were also induced by activation of protein kinase C with phorbol ester or by elevating the intracellular Ca2+ concentration with a Ca2+ ionophore. The Ca2+ effect was inhibited by KN-62, suggesting an induction through Ca2+/calmodulin-dependent kinase II. Inhibition of protein kinase C with GF109203X suppressed the carbachol-stimulated increase in mRNA levels of c-fos, fosB, and junB by approximately 70% but had only minor effects on the expression of c-jun and junD. On the other hand, preincubation with KN-62 attenuated the carbachol-induced increase in c-jun and junD expression by 70% but had no effect on c-fos, fosB, and junB mRNA levels. Simultaneous inhibition of both protein kinase C and Ca2+/calmodulin-dependent kinase II completely abolished the carbachol-stimulated expression of c-jun and junD, but c-fos, fosB, and junB were still expressed to a certain extent under this condition. Comparison of the inhibitory effects of GF109203X and Go 6976 suggests the involvement of classical protein kinase C isozymes in muscarinic receptor-stimulated expression of fos and jun genes. These results demonstrate that the muscarinic receptor-induced expression of individual fos and jun genes is regulated via different pathways, primarily protein kinase C or Ca2+/calmodulin-dependent kinase II.},
  author       = {Ding, Wei-Qun and Larsson, Christer and Alling, Christer},
  issn         = {1471-4159},
  keyword      = {Muscarinic receptor,Protein kinase C,Immediate-early genes,SH-SY5Y neuroblastoma cells,Ca2+/calmodulin-dependent kinase II},
  language     = {eng},
  number       = {4},
  pages        = {1722--1729},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Stimulation of muscarinic receptors induces expression of individual fos and jun genes through different transduction pathways},
  url          = {http://dx.doi.org/10.1046/j.1471-4159.1998.70041722.x},
  volume       = {70},
  year         = {1998},
}