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Ethanol exposure potentiates fosB and junB expression induced by muscarinic receptor stimulation in neuroblastoma SH-SY5Y cells

Ding, Wei-Qun; Fried, Ulrik LU ; Larsson, Christer LU and Alling, Christer LU (1998) In Alcoholism: Clinical and Experimental Research 22(1). p.225-230
Abstract
Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the... (More)
Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the expression of fosB and junB induced by activation of protein kinase C with phorbol ester. These results demonstrate that the muscarinic receptor-stimulated fosB and junB expression is sensitive to ethanol exposure in SH-SY5Y cells, suggesting that these genes participate in the regulation of neuronal function in response to chronic ethanol treatment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ethanol, fosBJunB, Muscarinic Receptor, Protein Kinase C
in
Alcoholism: Clinical and Experimental Research
volume
22
issue
1
pages
225 - 230
publisher
Wiley-Blackwell
external identifiers
  • pmid:9514311
  • scopus:0031884921
ISSN
0145-6008
DOI
10.1111/j.1530-0277.1998.tb03642.x
language
English
LU publication?
yes
id
8c0e93aa-9456-4738-97a7-41ecf90631d9 (old id 1112633)
date added to LUP
2008-07-10 10:48:37
date last changed
2017-05-14 03:25:22
@article{8c0e93aa-9456-4738-97a7-41ecf90631d9,
  abstract     = {Muscarinic receptor stimulation and activation of protein kinase C cause an increase in fosB and junB transcripts in human neuroblastoma SH-SY5Y cells. In this study, the effect of long-term ethanol exposure on these events was investigated. Carbachol-stimulated fosB and junB expression was elevated in ethanol-exposed cells compared with control cells. The potentiation was time- and dose-dependent on ethanol. Preincubation with muscarinic antagonists or protein kinase C inhibitor demonstrated that the carbachol-stimulated increase in fosB and junB mRNA levels was primarily mediated via M1 receptors and dependent on the activity of protein kinase C in both control and ethanol-exposed cells. Long-term ethanol exposure did not influence the expression of fosB and junB induced by activation of protein kinase C with phorbol ester. These results demonstrate that the muscarinic receptor-stimulated fosB and junB expression is sensitive to ethanol exposure in SH-SY5Y cells, suggesting that these genes participate in the regulation of neuronal function in response to chronic ethanol treatment.},
  author       = {Ding, Wei-Qun and Fried, Ulrik and Larsson, Christer and Alling, Christer},
  issn         = {0145-6008},
  keyword      = {Ethanol,fosBJunB,Muscarinic Receptor,Protein Kinase C},
  language     = {eng},
  number       = {1},
  pages        = {225--230},
  publisher    = {Wiley-Blackwell},
  series       = {Alcoholism: Clinical and Experimental Research},
  title        = {Ethanol exposure potentiates fosB and junB expression induced by muscarinic receptor stimulation in neuroblastoma SH-SY5Y cells},
  url          = {http://dx.doi.org/10.1111/j.1530-0277.1998.tb03642.x},
  volume       = {22},
  year         = {1998},
}