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Cerebral excitatory amino acids and Na+,K+-ATPase activity during resuscitation of severely hypoxic newborn piglets

Feet, B A; Gilland, E; Groenendaal, F; Brun, N C; Hellström-Westas, Lena LU ; Hagberg, H and Saugstad, O D (1998) In Acta Pædiatrica 87(8). p.889-895
Abstract
We tested the hypothesis that early brain recovery in hypoxic newborn piglets is improved by resuscitating with an O2 supply close to the minimum level required by the newborn piglet brain. Severely hypoxic 2-5-d-old anaesthetized piglets were randomly divided into three resuscitation groups: hypoxaemic (n = 8), 21% O2 (n = 8), and 100% O2 groups (n = 8). The hypoxaemic group was mechanically ventilated with 12-18% O2 adjusted to achieve a cerebral venous O2 saturation of 17-23% (baseline; 45 +/- 1%, mean +/- SEM). During the 2h resuscitation period, extracellular aspartate and glutamate concentrations in the cerebral striatum were higher during hypoxaemic resuscitation (p = 0.044 and p = 0.055, respectively) than during resuscitation with... (More)
We tested the hypothesis that early brain recovery in hypoxic newborn piglets is improved by resuscitating with an O2 supply close to the minimum level required by the newborn piglet brain. Severely hypoxic 2-5-d-old anaesthetized piglets were randomly divided into three resuscitation groups: hypoxaemic (n = 8), 21% O2 (n = 8), and 100% O2 groups (n = 8). The hypoxaemic group was mechanically ventilated with 12-18% O2 adjusted to achieve a cerebral venous O2 saturation of 17-23% (baseline; 45 +/- 1%, mean +/- SEM). During the 2h resuscitation period, extracellular aspartate and glutamate concentrations in the cerebral striatum were higher during hypoxaemic resuscitation (p = 0.044 and p = 0.055, respectively) than during resuscitation with 21% O2 or 100% O2, suggesting an unfavourable accumulation of potent excitotoxins during hypoxaemic resuscitation. The cell membrane Na+,K+-ATPase activity of cerebral cortical tissue after 2 h resuscitation was similar in the three groups (p = 0.30). In conclusion, hypoxaemic resuscitation did not normalize early cerebral metabolic recovery as efficiently as resuscitation with 21% O2 or 100% O2. Resuscitation with 21% O2 was as efficient as resuscitation with 100% O2 in this newborn piglet hypoxia model. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hypoxanthine, in vivo microdialysis, Asphyxia, oxygen, reoxygenation
in
Acta Pædiatrica
volume
87
issue
8
pages
889 - 895
publisher
Wiley-Blackwell Publishing Ltd
external identifiers
  • pmid:9736239
  • scopus:0031683655
ISSN
1651-2227
DOI
10.1111/j.1651-2227.1998.tb01557.x
language
English
LU publication?
yes
id
3fc4a31f-4cfa-4e41-86a3-c8256a8bb2c4 (old id 1112708)
alternative location
http://www3.interscience.wiley.com/cgi-bin/fulltext/119942684/PDFSTART
date added to LUP
2008-07-11 09:23:12
date last changed
2017-08-06 04:26:24
@article{3fc4a31f-4cfa-4e41-86a3-c8256a8bb2c4,
  abstract     = {We tested the hypothesis that early brain recovery in hypoxic newborn piglets is improved by resuscitating with an O2 supply close to the minimum level required by the newborn piglet brain. Severely hypoxic 2-5-d-old anaesthetized piglets were randomly divided into three resuscitation groups: hypoxaemic (n = 8), 21% O2 (n = 8), and 100% O2 groups (n = 8). The hypoxaemic group was mechanically ventilated with 12-18% O2 adjusted to achieve a cerebral venous O2 saturation of 17-23% (baseline; 45 +/- 1%, mean +/- SEM). During the 2h resuscitation period, extracellular aspartate and glutamate concentrations in the cerebral striatum were higher during hypoxaemic resuscitation (p = 0.044 and p = 0.055, respectively) than during resuscitation with 21% O2 or 100% O2, suggesting an unfavourable accumulation of potent excitotoxins during hypoxaemic resuscitation. The cell membrane Na+,K+-ATPase activity of cerebral cortical tissue after 2 h resuscitation was similar in the three groups (p = 0.30). In conclusion, hypoxaemic resuscitation did not normalize early cerebral metabolic recovery as efficiently as resuscitation with 21% O2 or 100% O2. Resuscitation with 21% O2 was as efficient as resuscitation with 100% O2 in this newborn piglet hypoxia model.},
  author       = {Feet, B A and Gilland, E and Groenendaal, F and Brun, N C and Hellström-Westas, Lena and Hagberg, H and Saugstad, O D},
  issn         = {1651-2227},
  keyword      = {hypoxanthine,in vivo microdialysis,Asphyxia,oxygen,reoxygenation},
  language     = {eng},
  number       = {8},
  pages        = {889--895},
  publisher    = {Wiley-Blackwell Publishing Ltd},
  series       = {Acta Pædiatrica},
  title        = {Cerebral excitatory amino acids and Na+,K+-ATPase activity during resuscitation of severely hypoxic newborn piglets},
  url          = {http://dx.doi.org/10.1111/j.1651-2227.1998.tb01557.x},
  volume       = {87},
  year         = {1998},
}