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Chromosomal organization of amplified chromosome 12 sequences in mesenchymal tumors detected by fluorescence in situ hybridization

Gisselsson, D LU ; Höglund, M LU ; Mertens, F LU ; Mitelman, F LU and Mandahl, N LU (1998) In Genes, Chromosomes and Cancer 23(3). p.203-212
Abstract

The chromosomal organization of amplified chromosome 12 sequences was studied with fluorescence in situ hybridization in six mesenchymal tumors: two osteosarcomas, one lipoma, two liposarcomas, and one fibrosarcoma. All except the fibrosarcoma contained ring and/or giant marker chromosomes. Amplification of chromosome 12 sequences, demonstrated with whole-chromosome paint in all cases, was confined to ring and giant marker chromosomes in four tumors. In one of the osteosarcomas and in the fibrosarcoma, amplified sequences were added to chromosome 12 and to chromosomes 10, 12, 18, and the Y chromosome, respectively. Hybridizations with single-copy probes demonstrated considerable inter- and intracellular variation in the arrangement of... (More)

The chromosomal organization of amplified chromosome 12 sequences was studied with fluorescence in situ hybridization in six mesenchymal tumors: two osteosarcomas, one lipoma, two liposarcomas, and one fibrosarcoma. All except the fibrosarcoma contained ring and/or giant marker chromosomes. Amplification of chromosome 12 sequences, demonstrated with whole-chromosome paint in all cases, was confined to ring and giant marker chromosomes in four tumors. In one of the osteosarcomas and in the fibrosarcoma, amplified sequences were added to chromosome 12 and to chromosomes 10, 12, 18, and the Y chromosome, respectively. Hybridizations with single-copy probes demonstrated considerable inter- and intracellular variation in the arrangement of chromosome 12 sequences in ring and marker chromosomes. Amplification of 12q13-15 sequences, predominantly from the HMGIC-MDM2 region, was detected in all cases, but the two osteosarcomas also contained amplification of 12p material. This finding, combined with results from previous studies, indicates that 12p amplification is a feature distinguishing osteosarcomas from adipose tissue tumors. A novel finding was the presence of positive signals for chromosome 12 alpha-satellite sequences in ring and marker chromosomes in four cases. Rod chromosomes carrying amplified material, in particular those that were relatively stable, frequently exhibited chromosome 12 negative terminal segments; two of these, present in two separate cases, were shown by C-banding to contain constitutive heterochromatin. The significant intercellular heterogeneity in the number and structure of rings and giant markers in a subset of mesenchymal tumors could be explained by continuous recombination through breakage-fusion-bridge cycles. If so, this process will continue until broken ends become stabilized, for example by acquisition of telomeric segments from other chromosomes.

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keywords
Adolescent, Adult, Chromosome Banding, Chromosome Painting, Chromosomes, Human, Pair 12, DNA, Satellite, Female, Fibrosarcoma, Gene Amplification, Humans, In Situ Hybridization, Fluorescence, Male, Middle Aged, Neoplasms, Adipose Tissue, Osteosarcoma, Physical Chromosome Mapping, Ring Chromosomes, Journal Article, Research Support, Non-U.S. Gov't
in
Genes, Chromosomes and Cancer
volume
23
issue
3
pages
10 pages
publisher
John Wiley & Sons
external identifiers
  • pmid:9790500
  • scopus:0345676507
ISSN
1045-2257
DOI
10.1002/(SICI)1098-2264(199811)23:3<203::AID-GCC1>3.0.CO;2-5
language
English
LU publication?
yes
id
e4b63be8-8957-4ba7-a9a3-4194e593aa9e (old id 1112894)
date added to LUP
2008-07-11 13:56:06
date last changed
2017-04-09 03:31:13
@article{e4b63be8-8957-4ba7-a9a3-4194e593aa9e,
  abstract     = {<p>The chromosomal organization of amplified chromosome 12 sequences was studied with fluorescence in situ hybridization in six mesenchymal tumors: two osteosarcomas, one lipoma, two liposarcomas, and one fibrosarcoma. All except the fibrosarcoma contained ring and/or giant marker chromosomes. Amplification of chromosome 12 sequences, demonstrated with whole-chromosome paint in all cases, was confined to ring and giant marker chromosomes in four tumors. In one of the osteosarcomas and in the fibrosarcoma, amplified sequences were added to chromosome 12 and to chromosomes 10, 12, 18, and the Y chromosome, respectively. Hybridizations with single-copy probes demonstrated considerable inter- and intracellular variation in the arrangement of chromosome 12 sequences in ring and marker chromosomes. Amplification of 12q13-15 sequences, predominantly from the HMGIC-MDM2 region, was detected in all cases, but the two osteosarcomas also contained amplification of 12p material. This finding, combined with results from previous studies, indicates that 12p amplification is a feature distinguishing osteosarcomas from adipose tissue tumors. A novel finding was the presence of positive signals for chromosome 12 alpha-satellite sequences in ring and marker chromosomes in four cases. Rod chromosomes carrying amplified material, in particular those that were relatively stable, frequently exhibited chromosome 12 negative terminal segments; two of these, present in two separate cases, were shown by C-banding to contain constitutive heterochromatin. The significant intercellular heterogeneity in the number and structure of rings and giant markers in a subset of mesenchymal tumors could be explained by continuous recombination through breakage-fusion-bridge cycles. If so, this process will continue until broken ends become stabilized, for example by acquisition of telomeric segments from other chromosomes.</p>},
  author       = {Gisselsson, D and Höglund, M and Mertens, F and Mitelman, F and Mandahl, N},
  issn         = {1045-2257},
  keyword      = {Adolescent,Adult,Chromosome Banding,Chromosome Painting,Chromosomes, Human, Pair 12,DNA, Satellite,Female,Fibrosarcoma,Gene Amplification,Humans,In Situ Hybridization, Fluorescence,Male,Middle Aged,Neoplasms, Adipose Tissue,Osteosarcoma,Physical Chromosome Mapping,Ring Chromosomes,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {3},
  pages        = {203--212},
  publisher    = {John Wiley & Sons},
  series       = {Genes, Chromosomes and Cancer},
  title        = {Chromosomal organization of amplified chromosome 12 sequences in mesenchymal tumors detected by fluorescence in situ hybridization},
  url          = {http://dx.doi.org/10.1002/(SICI)1098-2264(199811)23:3<203::AID-GCC1>3.0.CO;2-5},
  volume       = {23},
  year         = {1998},
}