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Antibody responses of rats after immunization with organic acid anhydrides as a model of predictive testing

Zhang, X D; Welinder, Hans LU ; Jönsson, Bo A LU and Skerfving, Staffan LU (1998) In Scandinavian Journal of Work, Environment and Health 24(3). p.220-227
Abstract
OBJECTIVES: The sensitizing properties of organic acid anhydrides (OAA) were evaluated in a rat model. METHODS: The development of specific immunoglobulin (Ig)E and Ig G in serum was investigated after immunization with 14 OAA and 3 OAA conjugates. Brown Norway rats were injected intradermally with 0.1 ml of 0.2 M OAA in liquid paraffin or 1.4 mg of rat serum albumin conjugate in saline. Serum samples were collected after 4 weeks. Antibodies were analyzed with enzyme-linked immunosorbent assay. RESULTS: The serum titers of specific Ig E after immunization with the different free OAA varied from <50 to 6400. The rats immunized with 4-methylphthalic anhydride exhibited the highest titers. The specificity of Ig E was demonstrated by... (More)
OBJECTIVES: The sensitizing properties of organic acid anhydrides (OAA) were evaluated in a rat model. METHODS: The development of specific immunoglobulin (Ig)E and Ig G in serum was investigated after immunization with 14 OAA and 3 OAA conjugates. Brown Norway rats were injected intradermally with 0.1 ml of 0.2 M OAA in liquid paraffin or 1.4 mg of rat serum albumin conjugate in saline. Serum samples were collected after 4 weeks. Antibodies were analyzed with enzyme-linked immunosorbent assay. RESULTS: The serum titers of specific Ig E after immunization with the different free OAA varied from <50 to 6400. The rats immunized with 4-methylphthalic anhydride exhibited the highest titers. The specificity of Ig E was demonstrated by enzyme-linked immunosorbent assay inhibition tests. A good correlation was observed between the Ig E and Ig G titers. Immunization with OAA conjugates showed results parallel to the findings for the free compounds. Importantly, the Ig E titers for the OAA agreed well with findings from guinea pigs and with literature data from epidemiologic studies of exposed workers. CONCLUSIONS: The present animal model may be a valuable tool for predicting the sensitizing potential of OAA and possibly the sensitizing potential of low-molecular-weight compounds in general. Furthermore, the antibody specificity of the haptens and the variations in the magnitude of the antibody titers indicate a valuable approach for studies of quantitative structure-activity relationships. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
occupational allergy, immunoglobulin G, animal model, immunoglobulin E, structure-activity relationship
in
Scandinavian Journal of Work, Environment and Health
volume
24
issue
3
pages
220 - 227
publisher
Finnish Institute of Occupational Health
external identifiers
  • pmid:9710375
  • scopus:0031871016
ISSN
0355-3140
language
English
LU publication?
yes
id
ae711adf-87eb-454b-b7de-9ac84ad4f1fb (old id 1112937)
date added to LUP
2008-07-11 15:19:03
date last changed
2017-01-01 05:02:43
@article{ae711adf-87eb-454b-b7de-9ac84ad4f1fb,
  abstract     = {OBJECTIVES: The sensitizing properties of organic acid anhydrides (OAA) were evaluated in a rat model. METHODS: The development of specific immunoglobulin (Ig)E and Ig G in serum was investigated after immunization with 14 OAA and 3 OAA conjugates. Brown Norway rats were injected intradermally with 0.1 ml of 0.2 M OAA in liquid paraffin or 1.4 mg of rat serum albumin conjugate in saline. Serum samples were collected after 4 weeks. Antibodies were analyzed with enzyme-linked immunosorbent assay. RESULTS: The serum titers of specific Ig E after immunization with the different free OAA varied from &lt;50 to 6400. The rats immunized with 4-methylphthalic anhydride exhibited the highest titers. The specificity of Ig E was demonstrated by enzyme-linked immunosorbent assay inhibition tests. A good correlation was observed between the Ig E and Ig G titers. Immunization with OAA conjugates showed results parallel to the findings for the free compounds. Importantly, the Ig E titers for the OAA agreed well with findings from guinea pigs and with literature data from epidemiologic studies of exposed workers. CONCLUSIONS: The present animal model may be a valuable tool for predicting the sensitizing potential of OAA and possibly the sensitizing potential of low-molecular-weight compounds in general. Furthermore, the antibody specificity of the haptens and the variations in the magnitude of the antibody titers indicate a valuable approach for studies of quantitative structure-activity relationships.},
  author       = {Zhang, X D and Welinder, Hans and Jönsson, Bo A and Skerfving, Staffan},
  issn         = {0355-3140},
  keyword      = {occupational allergy,immunoglobulin G,animal model,immunoglobulin E,structure-activity relationship},
  language     = {eng},
  number       = {3},
  pages        = {220--227},
  publisher    = {Finnish Institute of Occupational Health},
  series       = {Scandinavian Journal of Work, Environment and Health},
  title        = {Antibody responses of rats after immunization with organic acid anhydrides as a model of predictive testing},
  volume       = {24},
  year         = {1998},
}