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Inhibitory innervation of the guinea-pig urethra; roles of CO, NO and VIP

Werkström, Viktoria LU ; Alm, Per LU ; Persson, Katarina LU and Andersson, Karl-Erik LU (1998) In Journal of the Autonomic Nervous System 74(1). p.33-42
Abstract
The inhibitory innervation of guinea-pig urethral smooth muscle was investigated histochemically and functionally. The distribution of immunoreactivities to haem oxygenases (HO), neuronal NO synthase (nNOS), and vasoactive intestinal polypeptide (VIP) was studied, and the functional effects of the corresponding putative transmitters, CO, NO, and VIP, were assessed. HO-2 immunoreactivity was found in all nerve cell bodies of intramural ganglia, localized between smooth muscle bundles in the detrusor, bladder base and proximal urethra. About 70% of the ganglionic cell bodies were also NOS-immunoreactive (IR), whereas a minor part was VIP-IR. Some ganglion cells exhibiting tyrosine hydroxylase (TH) activity were demonstrated. Rich numbers of... (More)
The inhibitory innervation of guinea-pig urethral smooth muscle was investigated histochemically and functionally. The distribution of immunoreactivities to haem oxygenases (HO), neuronal NO synthase (nNOS), and vasoactive intestinal polypeptide (VIP) was studied, and the functional effects of the corresponding putative transmitters, CO, NO, and VIP, were assessed. HO-2 immunoreactivity was found in all nerve cell bodies of intramural ganglia, localized between smooth muscle bundles in the detrusor, bladder base and proximal urethra. About 70% of the ganglionic cell bodies were also NOS-immunoreactive (IR), whereas a minor part was VIP-IR. Some ganglion cells exhibiting tyrosine hydroxylase (TH) activity were demonstrated. Rich numbers of NOS-IR varicose nerve terminals could be found innervating the smooth muscle of the urethra, whereas VIP-IR terminals were less numerous. A rich number of TH-IR terminals were observed. The bladder showed a similar distribution of nerves, although only a few number of TH-IR nerves could be found. In bladder preparations exposed to sodium nitroprusside, cGMP-IR cells could be seen, forming an interconnecting network with long spindle-shaped processes. The cGMP-IR cells were especially abundant in the outer smooth muscle layers of the bladder, but less numerous in the urethra. In urethral strip preparations, electrical field stimulation evoked long-lasting frequency-dependent relaxations. The relaxations were not inhibited by the NO-synthesis inhibitor, L-NOARG, or enhanced by the NO-precursor, L-arginine. The haem precursor, 5-aminolevulinic acid (5-ALA), or the inhibitor of guanylate cyclase, ODQ, did not affect the urethral relaxations. Exogenously applied NO, SIN-1, and VIP relaxed the preparations by approximately 50%, whereas the relaxation evoked by exogenous CO was minor. These results suggest that CO probably is not involved in non-adrenergic, non-cholinergic inhibitory control of the guinea-pig urethra, where a non-NO/cGMP mediated relaxation seems to be predominant. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Carbon monoxide, Cyclic GMP, Haem oxygenase, Neurotransmission, Non-adrenergic non-cholinergic, Nitric oxide, Smooth muscle, Vasoactive intestinal polypeptide
in
Journal of the Autonomic Nervous System
volume
74
issue
1
pages
33 - 42
publisher
Elsevier
external identifiers
  • pmid:9858122
  • scopus:0032567131
ISSN
0165-1838
DOI
10.1016/S0165-1838(98)00135-0
language
English
LU publication?
yes
id
b6f74129-f2b6-404a-ac3c-c1bc57c9d860 (old id 1113099)
date added to LUP
2008-07-14 11:53:07
date last changed
2017-01-01 06:58:10
@article{b6f74129-f2b6-404a-ac3c-c1bc57c9d860,
  abstract     = {The inhibitory innervation of guinea-pig urethral smooth muscle was investigated histochemically and functionally. The distribution of immunoreactivities to haem oxygenases (HO), neuronal NO synthase (nNOS), and vasoactive intestinal polypeptide (VIP) was studied, and the functional effects of the corresponding putative transmitters, CO, NO, and VIP, were assessed. HO-2 immunoreactivity was found in all nerve cell bodies of intramural ganglia, localized between smooth muscle bundles in the detrusor, bladder base and proximal urethra. About 70% of the ganglionic cell bodies were also NOS-immunoreactive (IR), whereas a minor part was VIP-IR. Some ganglion cells exhibiting tyrosine hydroxylase (TH) activity were demonstrated. Rich numbers of NOS-IR varicose nerve terminals could be found innervating the smooth muscle of the urethra, whereas VIP-IR terminals were less numerous. A rich number of TH-IR terminals were observed. The bladder showed a similar distribution of nerves, although only a few number of TH-IR nerves could be found. In bladder preparations exposed to sodium nitroprusside, cGMP-IR cells could be seen, forming an interconnecting network with long spindle-shaped processes. The cGMP-IR cells were especially abundant in the outer smooth muscle layers of the bladder, but less numerous in the urethra. In urethral strip preparations, electrical field stimulation evoked long-lasting frequency-dependent relaxations. The relaxations were not inhibited by the NO-synthesis inhibitor, L-NOARG, or enhanced by the NO-precursor, L-arginine. The haem precursor, 5-aminolevulinic acid (5-ALA), or the inhibitor of guanylate cyclase, ODQ, did not affect the urethral relaxations. Exogenously applied NO, SIN-1, and VIP relaxed the preparations by approximately 50%, whereas the relaxation evoked by exogenous CO was minor. These results suggest that CO probably is not involved in non-adrenergic, non-cholinergic inhibitory control of the guinea-pig urethra, where a non-NO/cGMP mediated relaxation seems to be predominant.},
  author       = {Werkström, Viktoria and Alm, Per and Persson, Katarina and Andersson, Karl-Erik},
  issn         = {0165-1838},
  keyword      = {Carbon monoxide,Cyclic GMP,Haem oxygenase,Neurotransmission,Non-adrenergic non-cholinergic,Nitric oxide,Smooth muscle,Vasoactive intestinal polypeptide},
  language     = {eng},
  number       = {1},
  pages        = {33--42},
  publisher    = {Elsevier},
  series       = {Journal of the Autonomic Nervous System},
  title        = {Inhibitory innervation of the guinea-pig urethra; roles of CO, NO and VIP},
  url          = {http://dx.doi.org/10.1016/S0165-1838(98)00135-0},
  volume       = {74},
  year         = {1998},
}