Phenotype changes of the vascular smooth muscle cell regulate P2 receptor expression as measured by quantitative RT-PCR
(1998) In Biochemical and Biophysical Research Communications 248(3). p.864-870- Abstract
- Studies using selective agonists have suggested that the contractile effect of extracellular nucleotides, such as ATP and UTP, in blood vessels is mediated mainly by P2X1 receptors with a smaller contribution of P2Y receptors while the mitogenic effect is mediated by P2Y (P2Y1, P2Y2, P2Y4, and P2Y6) receptors with no effect of P2X1 receptors. This indicates a difference in P2 receptor expression between the contractile and the synthetic phenotype of the SMC. To measure the expression of mRNA for these receptors a competitive RT-PCR assay was developed that utilised synthetic RNA-competitors allowing determination of the number of mRNA copies for each receptor in the samples. In the synthetic phenotype the mitogenic P2Y1 and P2Y2 receptor... (More)
- Studies using selective agonists have suggested that the contractile effect of extracellular nucleotides, such as ATP and UTP, in blood vessels is mediated mainly by P2X1 receptors with a smaller contribution of P2Y receptors while the mitogenic effect is mediated by P2Y (P2Y1, P2Y2, P2Y4, and P2Y6) receptors with no effect of P2X1 receptors. This indicates a difference in P2 receptor expression between the contractile and the synthetic phenotype of the SMC. To measure the expression of mRNA for these receptors a competitive RT-PCR assay was developed that utilised synthetic RNA-competitors allowing determination of the number of mRNA copies for each receptor in the samples. In the synthetic phenotype the mitogenic P2Y1 and P2Y2 receptor transcripts were upregulated by 342- and 8-fold, respectively, while the contractile P2X1 receptor is totally downregulated and the P2Y4 and P2Y6 receptors were unchanged. This plasticity of the receptor expression may be important in the transition from the contractile to the synthetic SMC phenotype. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1113103
- author
- Erlinge, David
LU
; Hou, M ; Webb, T E ; Barnard, E A and Moller, S
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- UTP, ATP, competitive RT-PCR, P2 receptors, mRNA quantification, synthetic phenotype
- in
- Biochemical and Biophysical Research Communications
- volume
- 248
- issue
- 3
- pages
- 864 - 870
- publisher
- Elsevier
- external identifiers
-
- pmid:9704019
- scopus:0032581365
- ISSN
- 1090-2104
- DOI
- 10.1006/bbrc.1998.9083
- language
- English
- LU publication?
- yes
- id
- 24fa723b-44ee-4368-8f8b-91ee8d47c1ae (old id 1113103)
- date added to LUP
- 2016-04-01 16:42:45
- date last changed
- 2022-01-28 21:34:08
@article{24fa723b-44ee-4368-8f8b-91ee8d47c1ae, abstract = {{Studies using selective agonists have suggested that the contractile effect of extracellular nucleotides, such as ATP and UTP, in blood vessels is mediated mainly by P2X1 receptors with a smaller contribution of P2Y receptors while the mitogenic effect is mediated by P2Y (P2Y1, P2Y2, P2Y4, and P2Y6) receptors with no effect of P2X1 receptors. This indicates a difference in P2 receptor expression between the contractile and the synthetic phenotype of the SMC. To measure the expression of mRNA for these receptors a competitive RT-PCR assay was developed that utilised synthetic RNA-competitors allowing determination of the number of mRNA copies for each receptor in the samples. In the synthetic phenotype the mitogenic P2Y1 and P2Y2 receptor transcripts were upregulated by 342- and 8-fold, respectively, while the contractile P2X1 receptor is totally downregulated and the P2Y4 and P2Y6 receptors were unchanged. This plasticity of the receptor expression may be important in the transition from the contractile to the synthetic SMC phenotype.}}, author = {{Erlinge, David and Hou, M and Webb, T E and Barnard, E A and Moller, S}}, issn = {{1090-2104}}, keywords = {{UTP; ATP; competitive RT-PCR; P2 receptors; mRNA quantification; synthetic phenotype}}, language = {{eng}}, number = {{3}}, pages = {{864--870}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Phenotype changes of the vascular smooth muscle cell regulate P2 receptor expression as measured by quantitative RT-PCR}}, url = {{http://dx.doi.org/10.1006/bbrc.1998.9083}}, doi = {{10.1006/bbrc.1998.9083}}, volume = {{248}}, year = {{1998}}, }