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Phenotype changes of the vascular smooth muscle cell regulate P2 receptor expression as measured by quantitative RT-PCR

Erlinge, David LU ; Hou, M; Webb, T E; Barnard, E A and Moller, S (1998) In Biochemical and Biophysical Research Communications 248(3). p.864-870
Abstract
Studies using selective agonists have suggested that the contractile effect of extracellular nucleotides, such as ATP and UTP, in blood vessels is mediated mainly by P2X1 receptors with a smaller contribution of P2Y receptors while the mitogenic effect is mediated by P2Y (P2Y1, P2Y2, P2Y4, and P2Y6) receptors with no effect of P2X1 receptors. This indicates a difference in P2 receptor expression between the contractile and the synthetic phenotype of the SMC. To measure the expression of mRNA for these receptors a competitive RT-PCR assay was developed that utilised synthetic RNA-competitors allowing determination of the number of mRNA copies for each receptor in the samples. In the synthetic phenotype the mitogenic P2Y1 and P2Y2 receptor... (More)
Studies using selective agonists have suggested that the contractile effect of extracellular nucleotides, such as ATP and UTP, in blood vessels is mediated mainly by P2X1 receptors with a smaller contribution of P2Y receptors while the mitogenic effect is mediated by P2Y (P2Y1, P2Y2, P2Y4, and P2Y6) receptors with no effect of P2X1 receptors. This indicates a difference in P2 receptor expression between the contractile and the synthetic phenotype of the SMC. To measure the expression of mRNA for these receptors a competitive RT-PCR assay was developed that utilised synthetic RNA-competitors allowing determination of the number of mRNA copies for each receptor in the samples. In the synthetic phenotype the mitogenic P2Y1 and P2Y2 receptor transcripts were upregulated by 342- and 8-fold, respectively, while the contractile P2X1 receptor is totally downregulated and the P2Y4 and P2Y6 receptors were unchanged. This plasticity of the receptor expression may be important in the transition from the contractile to the synthetic SMC phenotype. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
UTP, ATP, competitive RT-PCR, P2 receptors, mRNA quantification, synthetic phenotype
in
Biochemical and Biophysical Research Communications
volume
248
issue
3
pages
864 - 870
publisher
Elsevier
external identifiers
  • pmid:9704019
  • scopus:0032581365
ISSN
1090-2104
DOI
10.1006/bbrc.1998.9083
language
English
LU publication?
yes
id
24fa723b-44ee-4368-8f8b-91ee8d47c1ae (old id 1113103)
date added to LUP
2008-07-14 11:55:53
date last changed
2017-08-06 04:37:09
@article{24fa723b-44ee-4368-8f8b-91ee8d47c1ae,
  abstract     = {Studies using selective agonists have suggested that the contractile effect of extracellular nucleotides, such as ATP and UTP, in blood vessels is mediated mainly by P2X1 receptors with a smaller contribution of P2Y receptors while the mitogenic effect is mediated by P2Y (P2Y1, P2Y2, P2Y4, and P2Y6) receptors with no effect of P2X1 receptors. This indicates a difference in P2 receptor expression between the contractile and the synthetic phenotype of the SMC. To measure the expression of mRNA for these receptors a competitive RT-PCR assay was developed that utilised synthetic RNA-competitors allowing determination of the number of mRNA copies for each receptor in the samples. In the synthetic phenotype the mitogenic P2Y1 and P2Y2 receptor transcripts were upregulated by 342- and 8-fold, respectively, while the contractile P2X1 receptor is totally downregulated and the P2Y4 and P2Y6 receptors were unchanged. This plasticity of the receptor expression may be important in the transition from the contractile to the synthetic SMC phenotype.},
  author       = {Erlinge, David and Hou, M and Webb, T E and Barnard, E A and Moller, S},
  issn         = {1090-2104},
  keyword      = {UTP,ATP,competitive RT-PCR,P2 receptors,mRNA quantification,synthetic phenotype},
  language     = {eng},
  number       = {3},
  pages        = {864--870},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Phenotype changes of the vascular smooth muscle cell regulate P2 receptor expression as measured by quantitative RT-PCR},
  url          = {http://dx.doi.org/10.1006/bbrc.1998.9083},
  volume       = {248},
  year         = {1998},
}