The nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester potentiates insulin secretion stimulated by glucose and L-arginine independently of its action on ATP-sensitive K+ channels
(1998) In Bioscience Reports 18(1). p.19-28- Abstract
- The nature of the action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on hormone release from isolated islets was investigated. We found that glucose-induced insulin release was potentiated by L-NAME in the absence or presence of diazoxide, a potent K+ATP channel opener, as well as in the presence of diazoxide plus a depolarizing concentration of K+. At a low, physiological glucose concentration L-NAME did not influence insulin secretion induced by K+ but inhibited glucagon secretion. L-arginine-induced insulin release was potentiated by L-NAME. This potentiation was observed also in the presence of K+ plus diazoxide. Further, glucagon release induced by L-arginine as well as by L-arginine plus K+... (More)
- The nature of the action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on hormone release from isolated islets was investigated. We found that glucose-induced insulin release was potentiated by L-NAME in the absence or presence of diazoxide, a potent K+ATP channel opener, as well as in the presence of diazoxide plus a depolarizing concentration of K+. At a low, physiological glucose concentration L-NAME did not influence insulin secretion induced by K+ but inhibited glucagon secretion. L-arginine-induced insulin release was potentiated by L-NAME. This potentiation was observed also in the presence of K+ plus diazoxide. Further, glucagon release induced by L-arginine as well as by L-arginine plus K+ and diazoxide was suppressed by L-NAME. The results strongly suggest that the L-NAME-induced potentiation of insulin secretion in response to glucose or L-arginine as well as the inhibitory effects on glucagon secretion are largely mediated by L-NAME directly suppressing islet NOS activity. Hence NO apparently affects insulin and glucagon secretion independently of membrane depolarization events. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1113492
- author
- Salehi, S Albert
LU
; Parandeh, Fariborz and Lundquist, Ingmar LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Insulin and glucagon secretion, isolated mouse islets, nitric oxide synthase, NG-nitro-L-arginine methyl ester, L-arginine, diazoxide, glucose, high K+
- in
- Bioscience Reports
- volume
- 18
- issue
- 1
- pages
- 19 - 28
- publisher
- Portland Press
- external identifiers
-
- pmid:9653515
- scopus:0031817046
- ISSN
- 0144-8463
- DOI
- 10.1023/A:1022288600348
- language
- English
- LU publication?
- yes
- id
- 4ff9cfc3-0f84-4f42-8fff-9983b27ecf51 (old id 1113492)
- date added to LUP
- 2016-04-01 15:18:01
- date last changed
- 2025-04-04 14:48:34
@article{4ff9cfc3-0f84-4f42-8fff-9983b27ecf51, abstract = {{The nature of the action of the nitric oxide synthase (NOS) inhibitor NG-nitro-L-arginine methyl ester (L-NAME) on hormone release from isolated islets was investigated. We found that glucose-induced insulin release was potentiated by L-NAME in the absence or presence of diazoxide, a potent K+ATP channel opener, as well as in the presence of diazoxide plus a depolarizing concentration of K+. At a low, physiological glucose concentration L-NAME did not influence insulin secretion induced by K+ but inhibited glucagon secretion. L-arginine-induced insulin release was potentiated by L-NAME. This potentiation was observed also in the presence of K+ plus diazoxide. Further, glucagon release induced by L-arginine as well as by L-arginine plus K+ and diazoxide was suppressed by L-NAME. The results strongly suggest that the L-NAME-induced potentiation of insulin secretion in response to glucose or L-arginine as well as the inhibitory effects on glucagon secretion are largely mediated by L-NAME directly suppressing islet NOS activity. Hence NO apparently affects insulin and glucagon secretion independently of membrane depolarization events.}}, author = {{Salehi, S Albert and Parandeh, Fariborz and Lundquist, Ingmar}}, issn = {{0144-8463}}, keywords = {{Insulin and glucagon secretion; isolated mouse islets; nitric oxide synthase; NG-nitro-L-arginine methyl ester; L-arginine; diazoxide; glucose; high K+}}, language = {{eng}}, number = {{1}}, pages = {{19--28}}, publisher = {{Portland Press}}, series = {{Bioscience Reports}}, title = {{The nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester potentiates insulin secretion stimulated by glucose and L-arginine independently of its action on ATP-sensitive K+ channels}}, url = {{http://dx.doi.org/10.1023/A:1022288600348}}, doi = {{10.1023/A:1022288600348}}, volume = {{18}}, year = {{1998}}, }