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The effect of selective phosphodiesterase inhibitors on mucociliary activity in the upper and lower airways in vitro

Cervin, Anders LU and Lindgren, Sam (1998) In Auris, Nasus, Larynx 25(3). p.269-276
Abstract
Bronchodilatory substances such as the phosphodisterase inhibitor (PDE-I) theophylline stimulate mucociliary activity. With the introduction of selective PDE-Is it has become possible to study the functional importance of each phosphodiesterase enzyme (PDE) concerning the regulation of the ciliary beat. The effects of rolipram (inhibiting a cAMP specific PDE (PDE4), milrinone (inhibiting a cGMP inhibited PDE (PDE3)) and zaprinast (inhibiting a cGMP specific PDE (PDE5)) were investigated in in vitro preparations from the rabbit maxillary sinus and trachea. Ciliary beat frequency (CBF) was measured with a photoelectrical method. In sinus mucosa all three compounds accelerated CBF. Milrinone (10(-5) M) by 22.6 +/- 5.3% (n = 6; P < 0.01),... (More)
Bronchodilatory substances such as the phosphodisterase inhibitor (PDE-I) theophylline stimulate mucociliary activity. With the introduction of selective PDE-Is it has become possible to study the functional importance of each phosphodiesterase enzyme (PDE) concerning the regulation of the ciliary beat. The effects of rolipram (inhibiting a cAMP specific PDE (PDE4), milrinone (inhibiting a cGMP inhibited PDE (PDE3)) and zaprinast (inhibiting a cGMP specific PDE (PDE5)) were investigated in in vitro preparations from the rabbit maxillary sinus and trachea. Ciliary beat frequency (CBF) was measured with a photoelectrical method. In sinus mucosa all three compounds accelerated CBF. Milrinone (10(-5) M) by 22.6 +/- 5.3% (n = 6; P < 0.01), rolipram (10(-5) M) by 29.7 +/- 5.7% (n = 7; P < 0.01), and zaprinast (10(-5) M) by 19.4 +/- 6.3% (n = 6; P < 0.05). In the tracheal specimens at a concentration of 10(-5) M, milrinone accelerated CBF by 27.5 +/- 9.0% (n = 7; < 0.05), rolipram by 11.6 +/- 2.8% (n = 6; P < 0.05) and zaprinast by 24.3 +/- 5.3% (n = 7; P < 0.01). Comparison of the effects in the upper and lower airways showed that at concentrations of 10(-5) and 10(-4) M rolipram was more effective in the upper than in the lower airways. The reverse was true of milrinone which concentrations of 10(-7) and 10(-6) M had a significant effect in tracheal specimens but not in sinus specimens. Zaprinast was equally effective in both the upper and lower airways. It is concluded that in both the upper and lower airways selective PDE-Is have an accelerating effect on the CBF that may be beneficial in the treatment of airway diseases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phosphodiesterase inhibitors, Rolipram, Milrinone, Zaprinast, Rabbit, Airway, Ciliary activity
in
Auris, Nasus, Larynx
volume
25
issue
3
pages
269 - 276
publisher
Elsevier
external identifiers
  • pmid:9799993
  • scopus:0031661087
ISSN
0385-8146
DOI
10.1016/S0385-8146(98)00010-8
language
English
LU publication?
yes
id
2d75ce62-9589-43c7-b4c1-74360541ac5d (old id 1113774)
date added to LUP
2008-07-16 11:12:43
date last changed
2017-01-01 04:31:38
@article{2d75ce62-9589-43c7-b4c1-74360541ac5d,
  abstract     = {Bronchodilatory substances such as the phosphodisterase inhibitor (PDE-I) theophylline stimulate mucociliary activity. With the introduction of selective PDE-Is it has become possible to study the functional importance of each phosphodiesterase enzyme (PDE) concerning the regulation of the ciliary beat. The effects of rolipram (inhibiting a cAMP specific PDE (PDE4), milrinone (inhibiting a cGMP inhibited PDE (PDE3)) and zaprinast (inhibiting a cGMP specific PDE (PDE5)) were investigated in in vitro preparations from the rabbit maxillary sinus and trachea. Ciliary beat frequency (CBF) was measured with a photoelectrical method. In sinus mucosa all three compounds accelerated CBF. Milrinone (10(-5) M) by 22.6 +/- 5.3% (n = 6; P &lt; 0.01), rolipram (10(-5) M) by 29.7 +/- 5.7% (n = 7; P &lt; 0.01), and zaprinast (10(-5) M) by 19.4 +/- 6.3% (n = 6; P &lt; 0.05). In the tracheal specimens at a concentration of 10(-5) M, milrinone accelerated CBF by 27.5 +/- 9.0% (n = 7; &lt; 0.05), rolipram by 11.6 +/- 2.8% (n = 6; P &lt; 0.05) and zaprinast by 24.3 +/- 5.3% (n = 7; P &lt; 0.01). Comparison of the effects in the upper and lower airways showed that at concentrations of 10(-5) and 10(-4) M rolipram was more effective in the upper than in the lower airways. The reverse was true of milrinone which concentrations of 10(-7) and 10(-6) M had a significant effect in tracheal specimens but not in sinus specimens. Zaprinast was equally effective in both the upper and lower airways. It is concluded that in both the upper and lower airways selective PDE-Is have an accelerating effect on the CBF that may be beneficial in the treatment of airway diseases.},
  author       = {Cervin, Anders and Lindgren, Sam},
  issn         = {0385-8146},
  keyword      = {Phosphodiesterase inhibitors,Rolipram,Milrinone,Zaprinast,Rabbit,Airway,Ciliary activity},
  language     = {eng},
  number       = {3},
  pages        = {269--276},
  publisher    = {Elsevier},
  series       = {Auris, Nasus, Larynx},
  title        = {The effect of selective phosphodiesterase inhibitors on mucociliary activity in the upper and lower airways in vitro},
  url          = {http://dx.doi.org/10.1016/S0385-8146(98)00010-8},
  volume       = {25},
  year         = {1998},
}