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Novel and classical protein kinase C isoforms have different functions in proliferation, survival and differentiation of neuroblastoma cells

Zeidman, Ruth LU ; Pettersson, Linda; Sailaja, P Ranga; Truedsson, Emma; Fagerstrom, Sofia; Påhlman, Sven LU and Larsson, Christer LU (1999) In International Journal of Cancer 81(3). p.494-501
Abstract
To elucidate the possibility of utilizing protein kinase C (PKC) isoforms as target genes in neuroblastoma therapy, 5 neuroblastoma cell lines and neuroblastoma tumor specimens were examined for PKC isoform expression pattern and the cell lines were analyzed for sensitivity to PKC inhibition. All cell lines [IMR-32, LAN-2, LAN-5, SH-SY5Y and SK-N-BE(2)] expressed alpha, betaII, delta and epsilon isoforms of PKC, while no PKCeta or theta protein was detected in any cell line. PKCgamma was found only in LAN-2 cells. PKCalpha, betaII and delta were detected in 5 neuroblastoma tumors and PKCepsilon in 4 out of 5 tumors. Exposure to the PKC inhibitors GF109203X, Go 6976 or Go 6983 caused a decrease whereas activation of PKC with... (More)
To elucidate the possibility of utilizing protein kinase C (PKC) isoforms as target genes in neuroblastoma therapy, 5 neuroblastoma cell lines and neuroblastoma tumor specimens were examined for PKC isoform expression pattern and the cell lines were analyzed for sensitivity to PKC inhibition. All cell lines [IMR-32, LAN-2, LAN-5, SH-SY5Y and SK-N-BE(2)] expressed alpha, betaII, delta and epsilon isoforms of PKC, while no PKCeta or theta protein was detected in any cell line. PKCgamma was found only in LAN-2 cells. PKCalpha, betaII and delta were detected in 5 neuroblastoma tumors and PKCepsilon in 4 out of 5 tumors. Exposure to the PKC inhibitors GF109203X, Go 6976 or Go 6983 caused a decrease whereas activation of PKC with 12-O-tetradecanoyl phorbol 13-acetate caused an increase in the number of neuroblastoma cells. The effect of Go 6976 was due to both inhibited proliferation and to increased apoptosis. While GF109203X suppressed neurite outgrowth induced by a growth factor combination, Go 6976 potentiated neurite outgrowth. Our data suggest a role for classical PKC isoforms in neuroblastoma growth and survival and for novel isoforms in neurite outgrowth. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Cancer
volume
81
issue
3
pages
494 - 501
publisher
John Wiley & Sons
external identifiers
  • pmid:10209967
  • scopus:0032891196
ISSN
0020-7136
DOI
10.1002/(SICI)1097-0215(19990505)81:3<494::AID-IJC26>3.0.CO;2-L
language
English
LU publication?
yes
id
b4d4c812-c67e-413f-8fd5-3654de74da1c (old id 1114114)
date added to LUP
2008-07-03 13:34:29
date last changed
2017-01-01 05:09:13
@article{b4d4c812-c67e-413f-8fd5-3654de74da1c,
  abstract     = {To elucidate the possibility of utilizing protein kinase C (PKC) isoforms as target genes in neuroblastoma therapy, 5 neuroblastoma cell lines and neuroblastoma tumor specimens were examined for PKC isoform expression pattern and the cell lines were analyzed for sensitivity to PKC inhibition. All cell lines [IMR-32, LAN-2, LAN-5, SH-SY5Y and SK-N-BE(2)] expressed alpha, betaII, delta and epsilon isoforms of PKC, while no PKCeta or theta protein was detected in any cell line. PKCgamma was found only in LAN-2 cells. PKCalpha, betaII and delta were detected in 5 neuroblastoma tumors and PKCepsilon in 4 out of 5 tumors. Exposure to the PKC inhibitors GF109203X, Go 6976 or Go 6983 caused a decrease whereas activation of PKC with 12-O-tetradecanoyl phorbol 13-acetate caused an increase in the number of neuroblastoma cells. The effect of Go 6976 was due to both inhibited proliferation and to increased apoptosis. While GF109203X suppressed neurite outgrowth induced by a growth factor combination, Go 6976 potentiated neurite outgrowth. Our data suggest a role for classical PKC isoforms in neuroblastoma growth and survival and for novel isoforms in neurite outgrowth.},
  author       = {Zeidman, Ruth and Pettersson, Linda and Sailaja, P Ranga and Truedsson, Emma and Fagerstrom, Sofia and Påhlman, Sven and Larsson, Christer},
  issn         = {0020-7136},
  language     = {eng},
  number       = {3},
  pages        = {494--501},
  publisher    = {John Wiley & Sons},
  series       = {International Journal of Cancer},
  title        = {Novel and classical protein kinase C isoforms have different functions in proliferation, survival and differentiation of neuroblastoma cells},
  url          = {http://dx.doi.org/10.1002/(SICI)1097-0215(19990505)81:3<494::AID-IJC26>3.0.CO;2-L},
  volume       = {81},
  year         = {1999},
}