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Expression profiling in cancer using cDNA microarrays

Khan, J; Saal, Lao LU ; Bittner, M L; Chen, Y; Trent, J M and Meltzer, P S (1999) In Electrophoresis 20(2). p.223-229
Abstract
Currently there are over 1,000,000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future... (More)
Currently there are over 1,000,000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future application of this powerful technique. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Electrophoresis
volume
20
issue
2
pages
223 - 229
publisher
John Wiley & Sons
external identifiers
  • pmid:10197427
  • scopus:0033012697
ISSN
0173-0835
DOI
10.1002/(SICI)1522-2683(19990201)20:2<223::AID-ELPS223>3.0.CO;2-A
language
English
LU publication?
yes
id
ce3f6ed8-03f9-4d1a-a46a-359201bd2022 (old id 1114488)
date added to LUP
2008-07-04 11:32:55
date last changed
2017-01-01 04:27:39
@article{ce3f6ed8-03f9-4d1a-a46a-359201bd2022,
  abstract     = {Currently there are over 1,000,000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future application of this powerful technique.},
  author       = {Khan, J and Saal, Lao and Bittner, M L and Chen, Y and Trent, J M and Meltzer, P S},
  issn         = {0173-0835},
  language     = {eng},
  number       = {2},
  pages        = {223--229},
  publisher    = {John Wiley & Sons},
  series       = {Electrophoresis},
  title        = {Expression profiling in cancer using cDNA microarrays},
  url          = {http://dx.doi.org/10.1002/(SICI)1522-2683(19990201)20:2<223::AID-ELPS223>3.0.CO;2-A},
  volume       = {20},
  year         = {1999},
}