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Functional effects of neuropeptide Y receptors on blood flow and nitric oxide levels in the human nose

Cervin, Anders LU ; Önnerfält, Jenny LU ; Edvinsson, Lars LU and Grundemar, Lars LU (1999) In American Journal of Respiratory and Critical Care Medicine 160(5). p.1724-1728
Abstract
The aim of this study was to examine dose-dependent effects of intranasal application of neuropeptide Y (NPY) on nasal mucosal blood flow, blood content, and intranasal nitric oxide (NO) concentration. Blood flow was measured by laser Doppler flowmetry (LDF) and blood content by rhinomanometry. Mucosal biopsies were taken for investigation of Y1 and Y2 receptor mRNA expression, using the reverse transcriptase-polymerase chain reaction (RT-PCR). Intranasal application of NPY evoked a dose-dependent reduction of nasal mucosal blood flow. Maximal vasoconstriction, seen at 12 nmol, was -37.5 +/- 6.2%, p < 0.05 (n = 9). The vasoconstrictive effect developed within 2 to 4 min and lasted > 17 min. NPY evoked a dose-dependent reduction of... (More)
The aim of this study was to examine dose-dependent effects of intranasal application of neuropeptide Y (NPY) on nasal mucosal blood flow, blood content, and intranasal nitric oxide (NO) concentration. Blood flow was measured by laser Doppler flowmetry (LDF) and blood content by rhinomanometry. Mucosal biopsies were taken for investigation of Y1 and Y2 receptor mRNA expression, using the reverse transcriptase-polymerase chain reaction (RT-PCR). Intranasal application of NPY evoked a dose-dependent reduction of nasal mucosal blood flow. Maximal vasoconstriction, seen at 12 nmol, was -37.5 +/- 6.2%, p < 0.05 (n = 9). The vasoconstrictive effect developed within 2 to 4 min and lasted > 17 min. NPY evoked a dose-dependent reduction of nasal airway resistance (NAR) on the ipsilateral side. Maximal decrease was -24.0 +/- 10.0% at 12 nmol, p < 0.05 (n = 9). There was a decrease in nasal NO production on the ipsilateral side after application of NPY 12 nmol (-7.4 +/- 1.2%, p < 0.05, n = 8). RT-PCR products corresponding to Y1 receptor but not Y2 receptor mRNA were obtained from biopsies of the nasal mucosa. In conclusion, NPY is a potent vasoconstrictor in the human nose reducing mucosal blood flow, as well as the blood content. The effect is probably mediated via Y1 receptors. NPY receptor agonists may prove beneficial in the treatment of the congested nose in allergic or vasomotor rhinitis. (Less)
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published
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keywords
Administration, Intranasal, Adult, Airway Resistance/drug effects, Dose-Response Relationship, Drug, Female, Humans, Laser-Doppler Flowmetry, Male, Nasal Mucosa/blood supply, Neuropeptide Y/administration & dosage, Nitric Oxide/metabolism, RNA, Messenger/analysis, Receptors, Neuropeptide Y/metabolism, Regional Blood Flow/drug effects, Reverse Transcriptase Polymerase Chain Reaction, Vasoconstriction/drug effects
in
American Journal of Respiratory and Critical Care Medicine
volume
160
issue
5
pages
5 pages
publisher
Am Thoracic Soc
external identifiers
  • pmid:10556147
  • scopus:0344718675
  • pmid:10556147
ISSN
1535-4970
DOI
10.1164/ajrccm.160.5.9902102
language
English
LU publication?
yes
id
ea1fbf38-80f9-4b0e-ae22-c9685ac610b5 (old id 1114708)
alternative location
http://ajrccm.atsjournals.org/cgi/content/full/160/5/1724
date added to LUP
2016-04-01 12:31:59
date last changed
2020-01-12 10:00:21
@article{ea1fbf38-80f9-4b0e-ae22-c9685ac610b5,
  abstract     = {The aim of this study was to examine dose-dependent effects of intranasal application of neuropeptide Y (NPY) on nasal mucosal blood flow, blood content, and intranasal nitric oxide (NO) concentration. Blood flow was measured by laser Doppler flowmetry (LDF) and blood content by rhinomanometry. Mucosal biopsies were taken for investigation of Y1 and Y2 receptor mRNA expression, using the reverse transcriptase-polymerase chain reaction (RT-PCR). Intranasal application of NPY evoked a dose-dependent reduction of nasal mucosal blood flow. Maximal vasoconstriction, seen at 12 nmol, was -37.5 +/- 6.2%, p &lt; 0.05 (n = 9). The vasoconstrictive effect developed within 2 to 4 min and lasted &gt; 17 min. NPY evoked a dose-dependent reduction of nasal airway resistance (NAR) on the ipsilateral side. Maximal decrease was -24.0 +/- 10.0% at 12 nmol, p &lt; 0.05 (n = 9). There was a decrease in nasal NO production on the ipsilateral side after application of NPY 12 nmol (-7.4 +/- 1.2%, p &lt; 0.05, n = 8). RT-PCR products corresponding to Y1 receptor but not Y2 receptor mRNA were obtained from biopsies of the nasal mucosa. In conclusion, NPY is a potent vasoconstrictor in the human nose reducing mucosal blood flow, as well as the blood content. The effect is probably mediated via Y1 receptors. NPY receptor agonists may prove beneficial in the treatment of the congested nose in allergic or vasomotor rhinitis.},
  author       = {Cervin, Anders and Önnerfält, Jenny and Edvinsson, Lars and Grundemar, Lars},
  issn         = {1535-4970},
  language     = {eng},
  number       = {5},
  pages        = {1724--1728},
  publisher    = {Am Thoracic Soc},
  series       = {American Journal of Respiratory and Critical Care Medicine},
  title        = {Functional effects of neuropeptide Y receptors on blood flow and nitric oxide levels in the human nose},
  url          = {http://dx.doi.org/10.1164/ajrccm.160.5.9902102},
  doi          = {10.1164/ajrccm.160.5.9902102},
  volume       = {160},
  year         = {1999},
}