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MAPKK-dependent growth factor-induced upregulation of P2Y2 receptors in vascular smooth muscle cells

Hou, Mingyan; Moller, Sebastian; Edvinsson, Lars LU and Erlinge, David LU (1999) In Biochemical and Biophysical Research Communications 258(3). p.648-652
Abstract
The ATP- and UTP-sensitive P2Y2 receptor which mediates both contractile and mitogenic effects has recently been shown to be upregulated in the synthetic phenotype of the vascular smooth muscle cell (VSMC). Using a competitive RT-PCR we demonstrate that the P2Y2 receptor mRNA is increased by fetal calf serum and other growth factors in a MAPKK-dependent way. This was confirmed at the functional level by examining UTP-stimulated release of intracellular Ca2+. Furthermore, the P2Y2 receptor mRNA is positively autoregulated by ATP and the mRNA is rapidly degraded with only 26% remaining after 1 h in the presence of actinomycin D. Our results indicate growth factor regulation and rapid turnover of the P2Y2 receptor mRNA, which may be of... (More)
The ATP- and UTP-sensitive P2Y2 receptor which mediates both contractile and mitogenic effects has recently been shown to be upregulated in the synthetic phenotype of the vascular smooth muscle cell (VSMC). Using a competitive RT-PCR we demonstrate that the P2Y2 receptor mRNA is increased by fetal calf serum and other growth factors in a MAPKK-dependent way. This was confirmed at the functional level by examining UTP-stimulated release of intracellular Ca2+. Furthermore, the P2Y2 receptor mRNA is positively autoregulated by ATP and the mRNA is rapidly degraded with only 26% remaining after 1 h in the presence of actinomycin D. Our results indicate growth factor regulation and rapid turnover of the P2Y2 receptor mRNA, which may be of importance in atherosclerosis and neointima formation after balloon angioplasty. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adenosine triphosphate, gene expression, P2Y receptor, competitive RT-PCR, mRNA quantification
in
Biochemical and Biophysical Research Communications
volume
258
issue
3
pages
648 - 652
publisher
Elsevier
external identifiers
  • pmid:10329439
  • scopus:0345161694
ISSN
1090-2104
DOI
10.1006/bbrc.1999.0676
language
English
LU publication?
yes
id
8a70d89e-b66c-4a49-8723-b5bd34990389 (old id 1114794)
date added to LUP
2008-07-04 16:37:54
date last changed
2017-01-01 07:22:21
@article{8a70d89e-b66c-4a49-8723-b5bd34990389,
  abstract     = {The ATP- and UTP-sensitive P2Y2 receptor which mediates both contractile and mitogenic effects has recently been shown to be upregulated in the synthetic phenotype of the vascular smooth muscle cell (VSMC). Using a competitive RT-PCR we demonstrate that the P2Y2 receptor mRNA is increased by fetal calf serum and other growth factors in a MAPKK-dependent way. This was confirmed at the functional level by examining UTP-stimulated release of intracellular Ca2+. Furthermore, the P2Y2 receptor mRNA is positively autoregulated by ATP and the mRNA is rapidly degraded with only 26% remaining after 1 h in the presence of actinomycin D. Our results indicate growth factor regulation and rapid turnover of the P2Y2 receptor mRNA, which may be of importance in atherosclerosis and neointima formation after balloon angioplasty.},
  author       = {Hou, Mingyan and Moller, Sebastian and Edvinsson, Lars and Erlinge, David},
  issn         = {1090-2104},
  keyword      = {adenosine triphosphate,gene expression,P2Y receptor,competitive RT-PCR,mRNA quantification},
  language     = {eng},
  number       = {3},
  pages        = {648--652},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {MAPKK-dependent growth factor-induced upregulation of P2Y2 receptors in vascular smooth muscle cells},
  url          = {http://dx.doi.org/10.1006/bbrc.1999.0676},
  volume       = {258},
  year         = {1999},
}