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Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure

Hou, Mingyan; Malmsjö, Malin LU ; Moller, Sebastian; Pantev, Emil LU ; Bergdahl, Anders; Zhao, X H; Sun, X Y; Hedner, T; Edvinsson, Lars LU and Erlinge, David LU (1999) In Life Sciences 65(11). p.1195-1206
Abstract
We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A... (More)
We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A prominent change was seen for the P2X1- and P2Y2-receptor mRNA levels which were increased 2.7-fold and 4.7-fold respectively in the myocardium from the left ventricle of CHF-rats. In contrast, the P2Y1-, P2Y4- and P2Y6-receptor mRNA levels were not significantly altered in CHF rats. In human myocard the P2X1-, P2Y1-, P2Y2-, P2Y6- and P2Y11-receptors were detected by RT-PCR in both right and left atria and ventricles, while the P2Y4-receptor band was weak or absent. In conclusion, most of the studied P2-receptors were expressed in both rat and human hearts. Furthermore, the P2X1- and P2Y2-receptor mRNA were upregulated in CHF, suggesting a pathophysiological role for these receptors in the development of heart failure. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
P2Y, P2X, receptor, quantitative RT-PCR, congestive heart failure
in
Life Sciences
volume
65
issue
11
pages
1195 - 1206
publisher
Elsevier
external identifiers
  • pmid:10503935
  • scopus:0033529419
ISSN
1879-0631
DOI
10.1016/S0024-3205(99)00353-7
language
English
LU publication?
yes
id
6912f71d-035f-40e5-a295-a74d687ae913 (old id 1114807)
date added to LUP
2008-07-04 16:44:38
date last changed
2017-01-15 03:37:35
@article{6912f71d-035f-40e5-a295-a74d687ae913,
  abstract     = {We wanted to study the expression of P2-receptors at the mRNA-level in the heart and if it is affected by congestive heart failure (CHF). To quantify the P2 receptor mRNA-expression we used a competitive RT-PCR protocol which is based on an internal RNA standard. The P2 receptor mRNA-expression was quantified in hearts from CHF rats and compared to sham-operated rats. Furthermore, the presence of receptor mRNA was studied in the myocardium from patients with heart failure. In the sham operated rats the G-protein coupled P2Y-receptors were expressed at a higher level than the ligand gated ion-channel receptor (P2X1). Among the P2Y-receptors the P2Y6-receptor was most abundantly expressed (P2Y6 > P2Y1 > P2Y2 = P2Y4 > P2X1). A prominent change was seen for the P2X1- and P2Y2-receptor mRNA levels which were increased 2.7-fold and 4.7-fold respectively in the myocardium from the left ventricle of CHF-rats. In contrast, the P2Y1-, P2Y4- and P2Y6-receptor mRNA levels were not significantly altered in CHF rats. In human myocard the P2X1-, P2Y1-, P2Y2-, P2Y6- and P2Y11-receptors were detected by RT-PCR in both right and left atria and ventricles, while the P2Y4-receptor band was weak or absent. In conclusion, most of the studied P2-receptors were expressed in both rat and human hearts. Furthermore, the P2X1- and P2Y2-receptor mRNA were upregulated in CHF, suggesting a pathophysiological role for these receptors in the development of heart failure.},
  author       = {Hou, Mingyan and Malmsjö, Malin and Moller, Sebastian and Pantev, Emil and Bergdahl, Anders and Zhao, X H and Sun, X Y and Hedner, T and Edvinsson, Lars and Erlinge, David},
  issn         = {1879-0631},
  keyword      = {P2Y,P2X,receptor,quantitative RT-PCR,congestive heart failure},
  language     = {eng},
  number       = {11},
  pages        = {1195--1206},
  publisher    = {Elsevier},
  series       = {Life Sciences},
  title        = {Increase in cardiac P2X1-and P2Y2-receptor mRNA levels in congestive heart failure},
  url          = {http://dx.doi.org/10.1016/S0024-3205(99)00353-7},
  volume       = {65},
  year         = {1999},
}