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Endothelial P2Y receptors induce hyperpolarisation of vascular smooth muscle by release of endothelium-derived hyperpolarising factor

Malmsjö, Malin LU ; Erlinge, David LU ; Högestätt, Edward LU and Zygmunt, Peter LU (1999) In European Journal of Pharmacology 364(2-3). p.169-173
Abstract
The effects of P2Y receptor agonists on smooth muscle membrane potential in isolated ring segments of rat mesenteric artery were examined by intracellular microelectrodes. In the presence of inhibitors of nitric oxide-synthase and cyclo-oxygenase, the selective P2Y1 receptor agonist adenosine 5'-O-thiodiphosphate (ADPbetaS) induced endothelium-dependent membrane hyperpolarisations, which were abolished by a combination of the K+ channel inhibitors charybdotoxin and apamin, providing direct evidence that ADPbetaS releases endothelium-derived hyperpolarising factor (EDHF). 2-MethylthioATP and ATP, each which stimulates both endothelial P2Y receptors and P2X receptors on the smooth muscle cells, also elicited hyperpolarisation, but only after... (More)
The effects of P2Y receptor agonists on smooth muscle membrane potential in isolated ring segments of rat mesenteric artery were examined by intracellular microelectrodes. In the presence of inhibitors of nitric oxide-synthase and cyclo-oxygenase, the selective P2Y1 receptor agonist adenosine 5'-O-thiodiphosphate (ADPbetaS) induced endothelium-dependent membrane hyperpolarisations, which were abolished by a combination of the K+ channel inhibitors charybdotoxin and apamin, providing direct evidence that ADPbetaS releases endothelium-derived hyperpolarising factor (EDHF). 2-MethylthioATP and ATP, each which stimulates both endothelial P2Y receptors and P2X receptors on the smooth muscle cells, also elicited hyperpolarisation, but only after desensitisation of P2X receptors with alphabeta-methylATP indicating that simultaneous activation of P2X receptors may counteract the action of EDHF. In conclusion, activation of endothelial P2Y receptors induce release of EDHF. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Membrane potential, K+ channel, Purinoceptor, Vascular endothelium
in
European Journal of Pharmacology
volume
364
issue
2-3
pages
169 - 173
publisher
Elsevier
external identifiers
  • pmid:9932720
  • scopus:0032953729
ISSN
1879-0712
DOI
10.1016/S0014-2999(98)00848-6
language
English
LU publication?
yes
id
6b0ae8d7-dce7-45e1-8b78-9015e6907925 (old id 1114833)
date added to LUP
2008-07-07 09:11:01
date last changed
2017-01-01 05:18:18
@article{6b0ae8d7-dce7-45e1-8b78-9015e6907925,
  abstract     = {The effects of P2Y receptor agonists on smooth muscle membrane potential in isolated ring segments of rat mesenteric artery were examined by intracellular microelectrodes. In the presence of inhibitors of nitric oxide-synthase and cyclo-oxygenase, the selective P2Y1 receptor agonist adenosine 5'-O-thiodiphosphate (ADPbetaS) induced endothelium-dependent membrane hyperpolarisations, which were abolished by a combination of the K+ channel inhibitors charybdotoxin and apamin, providing direct evidence that ADPbetaS releases endothelium-derived hyperpolarising factor (EDHF). 2-MethylthioATP and ATP, each which stimulates both endothelial P2Y receptors and P2X receptors on the smooth muscle cells, also elicited hyperpolarisation, but only after desensitisation of P2X receptors with alphabeta-methylATP indicating that simultaneous activation of P2X receptors may counteract the action of EDHF. In conclusion, activation of endothelial P2Y receptors induce release of EDHF.},
  author       = {Malmsjö, Malin and Erlinge, David and Högestätt, Edward and Zygmunt, Peter},
  issn         = {1879-0712},
  keyword      = {Membrane potential,K+ channel,Purinoceptor,Vascular endothelium},
  language     = {eng},
  number       = {2-3},
  pages        = {169--173},
  publisher    = {Elsevier},
  series       = {European Journal of Pharmacology},
  title        = {Endothelial P2Y receptors induce hyperpolarisation of vascular smooth muscle by release of endothelium-derived hyperpolarising factor},
  url          = {http://dx.doi.org/10.1016/S0014-2999(98)00848-6},
  volume       = {364},
  year         = {1999},
}