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Are neuronal markers and neocortical graft-host interface influenced by housing conditions in rats with cortical infarct cavity?

Zeng, J; Zhao, L R; Nordborg, C; Mattsson, Bengt LU and Johansson, Barbro LU (1999) In Brain Research Bulletin 48(2). p.165-171
Abstract
The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of... (More)
The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of the infarct cavity. In 8 of 18 transplants, 4 in each experimental group, part of the transplants protruded through the thin glial membrane that delineated the transplant-host interface into the adjacent host brain tissue. Microtubule associated protein 2 immunostained sections indicated bridging of dendrites in the host-transplant interface. Synaptophysin immunoreactivity was significantly higher in grafts than in contralateral cortex. However, graft morphology and neuronal marker immunoreactivity did not differ between rats housed in standard and activity stimulating cages. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cerebral infarction, GFAP, MAP2, Synaptophysin, Spontaneously hypertensive rats
in
Brain Research Bulletin
volume
48
issue
2
pages
165 - 171
publisher
Elsevier
external identifiers
  • pmid:10230707
  • scopus:0033555719
ISSN
0361-9230
DOI
10.1016/S0361-9230(98)00161-0
language
English
LU publication?
yes
id
b8b6469b-44ef-4b57-b761-3549e111e6ee (old id 1115192)
date added to LUP
2008-07-07 15:31:27
date last changed
2017-01-01 05:06:23
@article{b8b6469b-44ef-4b57-b761-3549e111e6ee,
  abstract     = {The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of the infarct cavity. In 8 of 18 transplants, 4 in each experimental group, part of the transplants protruded through the thin glial membrane that delineated the transplant-host interface into the adjacent host brain tissue. Microtubule associated protein 2 immunostained sections indicated bridging of dendrites in the host-transplant interface. Synaptophysin immunoreactivity was significantly higher in grafts than in contralateral cortex. However, graft morphology and neuronal marker immunoreactivity did not differ between rats housed in standard and activity stimulating cages.},
  author       = {Zeng, J and Zhao, L R and Nordborg, C and Mattsson, Bengt and Johansson, Barbro},
  issn         = {0361-9230},
  keyword      = {Cerebral infarction,GFAP,MAP2,Synaptophysin,Spontaneously hypertensive rats},
  language     = {eng},
  number       = {2},
  pages        = {165--171},
  publisher    = {Elsevier},
  series       = {Brain Research Bulletin},
  title        = {Are neuronal markers and neocortical graft-host interface influenced by housing conditions in rats with cortical infarct cavity?},
  url          = {http://dx.doi.org/10.1016/S0361-9230(98)00161-0},
  volume       = {48},
  year         = {1999},
}