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Chronic ulcers: a method for sampling and analysis of wound fluid

Schmidtchen, Artur LU (1999) In Acta Dermato-Venereologica 79(4). p.291-295
Abstract
There is growing evidence that chronic wounds show specific temporospatial patterns with respect to the expression of various molecules such as proteinases and their inhibitors. A careful analysis of molecules in the exudate of chronic wounds can be expected to provide useful information regarding events in the underlying tissue. In this connection there is a need for a method applicable to large amounts of patient material for collecting and analysing wound fluid from leg ulcers. A method of this sort is described. Wound fluid is accumulated for a defined period of time in Whatman GF/D filters. The filters are then extracted with isotonic buffer. Cells and other debris are centrifuged down and the supernatant is analysed further. The... (More)
There is growing evidence that chronic wounds show specific temporospatial patterns with respect to the expression of various molecules such as proteinases and their inhibitors. A careful analysis of molecules in the exudate of chronic wounds can be expected to provide useful information regarding events in the underlying tissue. In this connection there is a need for a method applicable to large amounts of patient material for collecting and analysing wound fluid from leg ulcers. A method of this sort is described. Wound fluid is accumulated for a defined period of time in Whatman GF/D filters. The filters are then extracted with isotonic buffer. Cells and other debris are centrifuged down and the supernatant is analysed further. The analysis can include electrophoresis on polyacrylamide gels, HPLC ion exchange chromatography and Western blotting techniques. The method will be useful for the sampling and analysis of wound fluid from patients with wounds of different aetiologies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Acta Dermato-Venereologica
volume
79
issue
4
pages
291 - 295
publisher
Medical Journals Limited
external identifiers
  • pmid:10429987
  • scopus:0032967744
ISSN
1651-2057
DOI
10.1080/000155599750010698
language
English
LU publication?
yes
id
5842b1a4-43f7-4c70-aa63-fbd44860e265 (old id 1115678)
date added to LUP
2008-07-08 11:28:57
date last changed
2017-07-02 04:29:55
@article{5842b1a4-43f7-4c70-aa63-fbd44860e265,
  abstract     = {There is growing evidence that chronic wounds show specific temporospatial patterns with respect to the expression of various molecules such as proteinases and their inhibitors. A careful analysis of molecules in the exudate of chronic wounds can be expected to provide useful information regarding events in the underlying tissue. In this connection there is a need for a method applicable to large amounts of patient material for collecting and analysing wound fluid from leg ulcers. A method of this sort is described. Wound fluid is accumulated for a defined period of time in Whatman GF/D filters. The filters are then extracted with isotonic buffer. Cells and other debris are centrifuged down and the supernatant is analysed further. The analysis can include electrophoresis on polyacrylamide gels, HPLC ion exchange chromatography and Western blotting techniques. The method will be useful for the sampling and analysis of wound fluid from patients with wounds of different aetiologies.},
  author       = {Schmidtchen, Artur},
  issn         = {1651-2057},
  language     = {eng},
  number       = {4},
  pages        = {291--295},
  publisher    = {Medical Journals Limited},
  series       = {Acta Dermato-Venereologica},
  title        = {Chronic ulcers: a method for sampling and analysis of wound fluid},
  url          = {http://dx.doi.org/10.1080/000155599750010698},
  volume       = {79},
  year         = {1999},
}