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Effects of cardioplegic flushing, storage, and reperfusion on coronary circulation in the pig

Budrikis, Algimantas; Liao, Qiuming LU ; Bolys, Ramunas; Westerlaken, Boris and Steen, Stig LU (1999) In Annals of Thoracic Surgery 67(5). p.1345-1349
Abstract
BACKGROUND: The aim of the study was to investigate how flush-perfusion of the heart with cold cardioplegic solution, 2 or 12 hours of cold ischemic storage, and 24 hours of reperfusion affect coronary endothelial function and coronary vascular resistance. METHODS: Porcine coronary arterial endothelial and smooth muscle function was studied in organ baths. An adult porcine working heart model was used to investigate coronary vascular resistance after 24 hours of reperfusion. RESULTS: Flushing the heart with 1 L of St. Thomas' cardioplegic solution, using a perfusion pressure of 60 to 65 mm Hg, significantly reduced endothelium-dependent relaxation. Flushing followed by 12 hours of storage gravely impaired endothelium-dependent relaxation,... (More)
BACKGROUND: The aim of the study was to investigate how flush-perfusion of the heart with cold cardioplegic solution, 2 or 12 hours of cold ischemic storage, and 24 hours of reperfusion affect coronary endothelial function and coronary vascular resistance. METHODS: Porcine coronary arterial endothelial and smooth muscle function was studied in organ baths. An adult porcine working heart model was used to investigate coronary vascular resistance after 24 hours of reperfusion. RESULTS: Flushing the heart with 1 L of St. Thomas' cardioplegic solution, using a perfusion pressure of 60 to 65 mm Hg, significantly reduced endothelium-dependent relaxation. Flushing followed by 12 hours of storage gravely impaired endothelium-dependent relaxation, and 24 hours of reperfusion worsened it still more. CONCLUSIONS: Flushing the heart with cold cardioplegic solution impairs endothelium-dependent relaxation, as does prolonged cold ischemic storage. Reperfusion of injured coronary endothelium may injure it still more. A correlation was found (p < 0.001) between high coronary vascular resistance and low endothelium-dependent relaxation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Annals of Thoracic Surgery
volume
67
issue
5
pages
1345 - 1349
publisher
Elsevier
external identifiers
  • pmid:10355409
  • scopus:0032989969
ISSN
1552-6259
language
English
LU publication?
yes
id
05010d01-888d-4263-87c9-7f4d0f5dbb2e (old id 1115974)
alternative location
http://ats.ctsnetjournals.org/cgi/content/abstract/67/5/1345
date added to LUP
2008-07-09 13:37:27
date last changed
2017-01-01 05:16:03
@article{05010d01-888d-4263-87c9-7f4d0f5dbb2e,
  abstract     = {BACKGROUND: The aim of the study was to investigate how flush-perfusion of the heart with cold cardioplegic solution, 2 or 12 hours of cold ischemic storage, and 24 hours of reperfusion affect coronary endothelial function and coronary vascular resistance. METHODS: Porcine coronary arterial endothelial and smooth muscle function was studied in organ baths. An adult porcine working heart model was used to investigate coronary vascular resistance after 24 hours of reperfusion. RESULTS: Flushing the heart with 1 L of St. Thomas' cardioplegic solution, using a perfusion pressure of 60 to 65 mm Hg, significantly reduced endothelium-dependent relaxation. Flushing followed by 12 hours of storage gravely impaired endothelium-dependent relaxation, and 24 hours of reperfusion worsened it still more. CONCLUSIONS: Flushing the heart with cold cardioplegic solution impairs endothelium-dependent relaxation, as does prolonged cold ischemic storage. Reperfusion of injured coronary endothelium may injure it still more. A correlation was found (p &lt; 0.001) between high coronary vascular resistance and low endothelium-dependent relaxation.},
  author       = {Budrikis, Algimantas and Liao, Qiuming and Bolys, Ramunas and Westerlaken, Boris and Steen, Stig},
  issn         = {1552-6259},
  language     = {eng},
  number       = {5},
  pages        = {1345--1349},
  publisher    = {Elsevier},
  series       = {Annals of Thoracic Surgery},
  title        = {Effects of cardioplegic flushing, storage, and reperfusion on coronary circulation in the pig},
  volume       = {67},
  year         = {1999},
}