Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein
Collin, Mattias LU
and Olsén, Arne
LU
(2000)
In Molecular Microbiology
36(6).
p.1306-1318
- Abstract
- In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We also show that MC25 secretes a 40 kDa zymogen, having a conformation different from that secreted by wild-type bacteria. We provide data showing that the cleavage site is not blocked but, presumably, the active site is. This suggests that M protein, when anchored to the cell wall, is involved in the unfolding of the zymogen and generation of a... (More)
- In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We also show that MC25 secretes a 40 kDa zymogen, having a conformation different from that secreted by wild-type bacteria. We provide data showing that the cleavage site is not blocked but, presumably, the active site is. This suggests that M protein, when anchored to the cell wall, is involved in the unfolding of the zymogen and generation of a mature cysteine proteinase that can be activated under reducing conditions. Our data add new aspects to the interaction between two important virulence factors of S. pyogenes, the streptococcal cysteine proteinase and M protein. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1116172
- author
- Collin, Mattias
LU
and Olsén, Arne
LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Microbiology
- volume
- 36
- issue
- 6
- pages
- 1306 - 1318
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:10931281
- scopus:0033923852
- ISSN
- 1365-2958
- DOI
- 10.1046/j.1365-2958.2000.01942.x
- language
- English
- LU publication?
- yes
- id
- 8d9cb058-d78e-4a27-b029-7e7ed9b943c7 (old id 1116172)
- date added to LUP
- 2016-04-01 11:40:52
- date last changed
- 2022-01-26 08:39:19
@article{8d9cb058-d78e-4a27-b029-7e7ed9b943c7,
abstract = {{In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We also show that MC25 secretes a 40 kDa zymogen, having a conformation different from that secreted by wild-type bacteria. We provide data showing that the cleavage site is not blocked but, presumably, the active site is. This suggests that M protein, when anchored to the cell wall, is involved in the unfolding of the zymogen and generation of a mature cysteine proteinase that can be activated under reducing conditions. Our data add new aspects to the interaction between two important virulence factors of S. pyogenes, the streptococcal cysteine proteinase and M protein.}},
author = {{Collin, Mattias and Olsén, Arne}},
issn = {{1365-2958}},
language = {{eng}},
number = {{6}},
pages = {{1306--1318}},
publisher = {{Wiley-Blackwell}},
series = {{Molecular Microbiology}},
title = {{Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein}},
url = {{http://dx.doi.org/10.1046/j.1365-2958.2000.01942.x}},
doi = {{10.1046/j.1365-2958.2000.01942.x}},
volume = {{36}},
year = {{2000}},
}