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Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein

Collin, Mattias LU and Olsén, Arne LU (2000) In Molecular Microbiology 36(6). p.1306-1318
Abstract
In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We also show that MC25 secretes a 40 kDa zymogen, having a conformation different from that secreted by wild-type bacteria. We provide data showing that the cleavage site is not blocked but, presumably, the active site is. This suggests that M protein, when anchored to the cell wall, is involved in the unfolding of the zymogen and generation of a... (More)
In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We also show that MC25 secretes a 40 kDa zymogen, having a conformation different from that secreted by wild-type bacteria. We provide data showing that the cleavage site is not blocked but, presumably, the active site is. This suggests that M protein, when anchored to the cell wall, is involved in the unfolding of the zymogen and generation of a mature cysteine proteinase that can be activated under reducing conditions. Our data add new aspects to the interaction between two important virulence factors of S. pyogenes, the streptococcal cysteine proteinase and M protein. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Microbiology
volume
36
issue
6
pages
1306 - 1318
publisher
Wiley-Blackwell
external identifiers
  • pmid:10931281
  • scopus:0033923852
ISSN
1365-2958
DOI
10.1046/j.1365-2958.2000.01942.x
language
English
LU publication?
yes
id
8d9cb058-d78e-4a27-b029-7e7ed9b943c7 (old id 1116172)
date added to LUP
2008-07-01 09:18:55
date last changed
2017-10-01 03:37:23
@article{8d9cb058-d78e-4a27-b029-7e7ed9b943c7,
  abstract     = {In the present study, we have generated a mutant strain of Streptococcus pyogenes, MC25, which lacks M protein on its surface, and we demonstrate that this strain is unable to generate a mature 28 kDa cysteine proteinase. Furthermore, we show that S. pyogenes bacteria of M1 serotype are dependent on cell wall-anchored M protein to cleave the secreted zymogen into a mature cysteine proteinase. We also show that MC25 secretes a 40 kDa zymogen, having a conformation different from that secreted by wild-type bacteria. We provide data showing that the cleavage site is not blocked but, presumably, the active site is. This suggests that M protein, when anchored to the cell wall, is involved in the unfolding of the zymogen and generation of a mature cysteine proteinase that can be activated under reducing conditions. Our data add new aspects to the interaction between two important virulence factors of S. pyogenes, the streptococcal cysteine proteinase and M protein.},
  author       = {Collin, Mattias and Olsén, Arne},
  issn         = {1365-2958},
  language     = {eng},
  number       = {6},
  pages        = {1306--1318},
  publisher    = {Wiley-Blackwell},
  series       = {Molecular Microbiology},
  title        = {Generation of a mature streptococcal cysteine proteinase is dependent on cell wall-anchored M1 protein},
  url          = {http://dx.doi.org/10.1046/j.1365-2958.2000.01942.x},
  volume       = {36},
  year         = {2000},
}