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Protein kinase C beta1 is implicated in the regulation of neuroblastoma cell growth and proliferation

Svensson, Karin; Zeidman, Ruth LU ; Trollér, Ulrika LU ; Schultz, Anna LU and Larsson, Christer LU (2000) In Cell Growth & Differentiation 11(12). p.641-648
Abstract
To investigate a putative involvement of protein kinase C (PKC) isoforms in supporting neuroblastoma cell proliferation, SK-N-BE(2) neuroblastoma cells were transfected with expression vectors coding for the C2 and V5 regions from different PKC isoforms. These structures have been suggested to inhibit the activity of their corresponding PKC isoform. The PKC fragments were fused to enhanced green fluorescent protein to facilitate the detection of transfected cells. Expression of the C2 domain from a classical PKC isoform (PKCalpha), but not of C2 domains from novel PKCdelta or PKCepsilon, suppressed the number of neuroblastoma cells positive for cyclin A and bromodeoxyuridine incorporation. This indicates a role for a classical isoform in... (More)
To investigate a putative involvement of protein kinase C (PKC) isoforms in supporting neuroblastoma cell proliferation, SK-N-BE(2) neuroblastoma cells were transfected with expression vectors coding for the C2 and V5 regions from different PKC isoforms. These structures have been suggested to inhibit the activity of their corresponding PKC isoform. The PKC fragments were fused to enhanced green fluorescent protein to facilitate the detection of transfected cells. Expression of the C2 domain from a classical PKC isoform (PKCalpha), but not of C2 domains from novel PKCdelta or PKCepsilon, suppressed the number of neuroblastoma cells positive for cyclin A and bromodeoxyuridine incorporation. This indicates a role for a classical isoform in regulating proliferation of these cells. Among the V5 fragments from PKCalpha, PKCbetaI, and PKCbetaII, the PKCbetaI V5 had the most suppressive effect on proliferation markers, and this fragment also displaced PKCbetaI from the nucleus. Furthermore, a PKCbeta-specific inhibitor, LY379196, suppressed the phorbol ester- and serum-supported growth of neuroblastoma cells. There was a marked enhancement by LY379196 of the growth-suppressive and/or cytotoxic effects of paclitaxel and vincristine. These results indicate that PKCbetaI has a positive effect on the growth and proliferation of neuroblastoma cells and demonstrate that inhibition of PKCbeta may be used to enhance the effect of microtubule-interacting anticancer agents on neuroblastoma cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell Growth & Differentiation
volume
11
issue
12
pages
641 - 648
publisher
American Association for Cancer Research
external identifiers
  • pmid:11149599
ISSN
1044-9523
language
English
LU publication?
yes
id
e4f5d1d6-242a-4e7a-b722-7911e786e485 (old id 1116174)
alternative location
http://cgd.aacrjournals.org/cgi/content/full/11/12/641
date added to LUP
2008-07-01 09:22:07
date last changed
2016-04-16 04:07:53
@article{e4f5d1d6-242a-4e7a-b722-7911e786e485,
  abstract     = {To investigate a putative involvement of protein kinase C (PKC) isoforms in supporting neuroblastoma cell proliferation, SK-N-BE(2) neuroblastoma cells were transfected with expression vectors coding for the C2 and V5 regions from different PKC isoforms. These structures have been suggested to inhibit the activity of their corresponding PKC isoform. The PKC fragments were fused to enhanced green fluorescent protein to facilitate the detection of transfected cells. Expression of the C2 domain from a classical PKC isoform (PKCalpha), but not of C2 domains from novel PKCdelta or PKCepsilon, suppressed the number of neuroblastoma cells positive for cyclin A and bromodeoxyuridine incorporation. This indicates a role for a classical isoform in regulating proliferation of these cells. Among the V5 fragments from PKCalpha, PKCbetaI, and PKCbetaII, the PKCbetaI V5 had the most suppressive effect on proliferation markers, and this fragment also displaced PKCbetaI from the nucleus. Furthermore, a PKCbeta-specific inhibitor, LY379196, suppressed the phorbol ester- and serum-supported growth of neuroblastoma cells. There was a marked enhancement by LY379196 of the growth-suppressive and/or cytotoxic effects of paclitaxel and vincristine. These results indicate that PKCbetaI has a positive effect on the growth and proliferation of neuroblastoma cells and demonstrate that inhibition of PKCbeta may be used to enhance the effect of microtubule-interacting anticancer agents on neuroblastoma cells.},
  author       = {Svensson, Karin and Zeidman, Ruth and Trollér, Ulrika and Schultz, Anna and Larsson, Christer},
  issn         = {1044-9523},
  language     = {eng},
  number       = {12},
  pages        = {641--648},
  publisher    = {American Association for Cancer Research},
  series       = {Cell Growth & Differentiation},
  title        = {Protein kinase C beta1 is implicated in the regulation of neuroblastoma cell growth and proliferation},
  volume       = {11},
  year         = {2000},
}