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IS1294, a DNA element that transposes by RC transposition

Tavakoli, N; Comanducci, A; Dodd, H M; Lett, M C; Albiger, Barbara LU and Bennett, P (2000) In Plasmid 44(1). p.66-84
Abstract
IS1294, found on the ColD-like resistance plasmid pUB2380, is IS91-like. It is an active 1.7-kb insertion sequence that lacks terminal inverted repeats, displays insertion-site specificity, and does not generate direct repeats of the target site. The element has one large open reading frame, tnp(1294), encoding a transposase of 351 amino acids, related to members of the REP family of replication proteins used by RC-plasmids of gram-positive bacteria. IS1294 transposes using rolling-circle replication, initiated at one end of the element, oriIS, and terminated at the other, terIS. oriIS and terIS are highly conserved among like IS elements. oriIS resembles the leading strand replication origins of RC-plasmids; terIS resembles a... (More)
IS1294, found on the ColD-like resistance plasmid pUB2380, is IS91-like. It is an active 1.7-kb insertion sequence that lacks terminal inverted repeats, displays insertion-site specificity, and does not generate direct repeats of the target site. The element has one large open reading frame, tnp(1294), encoding a transposase of 351 amino acids, related to members of the REP family of replication proteins used by RC-plasmids of gram-positive bacteria. IS1294 transposes using rolling-circle replication, initiated at one end of the element, oriIS, and terminated at the other, terIS. oriIS and terIS are highly conserved among like IS elements. oriIS resembles the leading strand replication origins of RC-plasmids; terIS resembles a rho-independent transcription terminator. IS1294 mediates not only its own transposition, but also sequences adjacent to terIS. A transposition model for IS1294 and related elements, involving rolling-circle replication and single-strand DNA intermediates, is presented. (Less)
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Plasmid
volume
44
issue
1
pages
66 - 84
publisher
Academic Press
external identifiers
  • pmid:10873528
  • scopus:0033912362
ISSN
0147-619X
DOI
10.1006/plas.1999.1460
language
English
LU publication?
no
id
013bd037-2823-456f-8521-3564ec23570d (old id 1116300)
date added to LUP
2008-07-01 11:26:10
date last changed
2017-03-26 03:42:35
@article{013bd037-2823-456f-8521-3564ec23570d,
  abstract     = {IS1294, found on the ColD-like resistance plasmid pUB2380, is IS91-like. It is an active 1.7-kb insertion sequence that lacks terminal inverted repeats, displays insertion-site specificity, and does not generate direct repeats of the target site. The element has one large open reading frame, tnp(1294), encoding a transposase of 351 amino acids, related to members of the REP family of replication proteins used by RC-plasmids of gram-positive bacteria. IS1294 transposes using rolling-circle replication, initiated at one end of the element, oriIS, and terminated at the other, terIS. oriIS and terIS are highly conserved among like IS elements. oriIS resembles the leading strand replication origins of RC-plasmids; terIS resembles a rho-independent transcription terminator. IS1294 mediates not only its own transposition, but also sequences adjacent to terIS. A transposition model for IS1294 and related elements, involving rolling-circle replication and single-strand DNA intermediates, is presented.},
  author       = {Tavakoli, N and Comanducci, A and Dodd, H M and Lett, M C and Albiger, Barbara and Bennett, P},
  issn         = {0147-619X},
  language     = {eng},
  number       = {1},
  pages        = {66--84},
  publisher    = {Academic Press},
  series       = {Plasmid},
  title        = {IS1294, a DNA element that transposes by RC transposition},
  url          = {http://dx.doi.org/10.1006/plas.1999.1460},
  volume       = {44},
  year         = {2000},
}