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Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor

Tryselius, Ylva LU ; Nilsson, Niclas LU ; Kotarsky, Knut LU ; Olde, Björn LU and Owman, Christer LU (2000) In Biochemical and Biophysical Research Communications 274(2). p.377-382
Abstract
By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa... (More)
By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
leukotriene B4, BLTR2, BLTR, calcium assay, 7TM receptor
in
Biochemical and Biophysical Research Communications
volume
274
issue
2
pages
377 - 382
publisher
Elsevier
external identifiers
  • pmid:10913346
  • scopus:0034596213
ISSN
1090-2104
DOI
10.1006/bbrc.2000.3152
language
English
LU publication?
yes
id
91df6b61-d735-453a-9c3b-e35c088ebe9f (old id 1116321)
date added to LUP
2016-04-01 15:18:32
date last changed
2022-03-22 03:47:31
@article{91df6b61-d735-453a-9c3b-e35c088ebe9f,
  abstract     = {{By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2.}},
  author       = {{Tryselius, Ylva and Nilsson, Niclas and Kotarsky, Knut and Olde, Björn and Owman, Christer}},
  issn         = {{1090-2104}},
  keywords     = {{leukotriene B4; BLTR2; BLTR; calcium assay; 7TM receptor}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{377--382}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor}},
  url          = {{http://dx.doi.org/10.1006/bbrc.2000.3152}},
  doi          = {{10.1006/bbrc.2000.3152}},
  volume       = {{274}},
  year         = {{2000}},
}