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Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor

Tryselius, Ylva LU ; Nilsson, Niclas LU ; Kotarsky, Knut LU ; Olde, Björn LU and Owman, Christer LU (2000) In Biochemical and Biophysical Research Communications 274(2). p.377-382
Abstract
By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa... (More)
By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
leukotriene B4, BLTR2, BLTR, calcium assay, 7TM receptor
in
Biochemical and Biophysical Research Communications
volume
274
issue
2
pages
377 - 382
publisher
Elsevier
external identifiers
  • pmid:10913346
  • scopus:0034596213
ISSN
1090-2104
DOI
10.1006/bbrc.2000.3152
language
English
LU publication?
yes
id
91df6b61-d735-453a-9c3b-e35c088ebe9f (old id 1116321)
date added to LUP
2008-07-01 11:32:10
date last changed
2017-07-30 04:25:31
@article{91df6b61-d735-453a-9c3b-e35c088ebe9f,
  abstract     = {By homology screening using BLAST searches of expressed sequence tags (ESTs), we have found a previously unidentified cDNA encoding a putative seven-transmembrane receptor with highest similarity to the leukotriene B(4) receptor, BLTR. Analysis of calcium flow in transfected cells, along with sequence analysis, revealed that the EST encoded a functionally inactive protein, lacking the segment corresponding to the C-terminal part of the putative receptor protein. The missing segment was obtained by PCR amplification of a human leukocyte cDNA library and ligated to the truncated EST cDNA. The novel cDNA encodes a full-length receptor with 39% identity to the previously cloned BLTR. Studies of intracellular calcium flow of transfected HeLa cells exposed to various leukotrienes showed that also the novel BLTR-like receptor can be activated by leukotriene B(4), and it is therefore tentatively named BLTR2.},
  author       = {Tryselius, Ylva and Nilsson, Niclas and Kotarsky, Knut and Olde, Björn and Owman, Christer},
  issn         = {1090-2104},
  keyword      = {leukotriene B4,BLTR2,BLTR,calcium assay,7TM receptor},
  language     = {eng},
  number       = {2},
  pages        = {377--382},
  publisher    = {Elsevier},
  series       = {Biochemical and Biophysical Research Communications},
  title        = {Cloning and characterization of cDNA encoding a novel human leukotriene B(4) receptor},
  url          = {http://dx.doi.org/10.1006/bbrc.2000.3152},
  volume       = {274},
  year         = {2000},
}