Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Purification of rabbit lacrimal gland plasma membranes by aqueous two-phase affinity partitioning

Ekblad, Lars LU orcid ; Jergil, Bengt LU and Gierow, J Peter (2000) In Journal of Chromatography. B 743(1-2). p.397-401
Abstract
We describe the purification of lacrimal gland plasma membranes by affinity partitioning using a two-phase system containing polyethylene glycol and dextran in which wheat germ agglutinin conjugated to dextran is used as affinity ligand. When partitioning a microsomal fraction, the plasma membrane marker 5'-nucleotidase was obtained in the affinity ligand-containing bottom phase, whereas the endoplasmic reticulum marker NADH-ferricyanide reductase remained in the top phase. The affinity partitioning behaviour of components involved in exocytosis and cellular signalling was also examined.
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phosphatidylinositol 4-kinase
in
Journal of Chromatography. B
volume
743
issue
1-2
pages
397 - 401
publisher
Elsevier
external identifiers
  • pmid:10942310
  • scopus:0034705689
ISSN
1387-2273
DOI
10.1016/S0378-4347(00)00152-3
language
English
LU publication?
yes
id
3399b16f-5d44-4011-9650-0ae9859f51d8 (old id 1116765)
date added to LUP
2016-04-01 16:49:47
date last changed
2022-01-28 22:28:33
@article{3399b16f-5d44-4011-9650-0ae9859f51d8,
  abstract     = {{We describe the purification of lacrimal gland plasma membranes by affinity partitioning using a two-phase system containing polyethylene glycol and dextran in which wheat germ agglutinin conjugated to dextran is used as affinity ligand. When partitioning a microsomal fraction, the plasma membrane marker 5'-nucleotidase was obtained in the affinity ligand-containing bottom phase, whereas the endoplasmic reticulum marker NADH-ferricyanide reductase remained in the top phase. The affinity partitioning behaviour of components involved in exocytosis and cellular signalling was also examined.}},
  author       = {{Ekblad, Lars and Jergil, Bengt and Gierow, J Peter}},
  issn         = {{1387-2273}},
  keywords     = {{Phosphatidylinositol 4-kinase}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{397--401}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography. B}},
  title        = {{Purification of rabbit lacrimal gland plasma membranes by aqueous two-phase affinity partitioning}},
  url          = {{http://dx.doi.org/10.1016/S0378-4347(00)00152-3}},
  doi          = {{10.1016/S0378-4347(00)00152-3}},
  volume       = {{743}},
  year         = {{2000}},
}