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Purification of rabbit lacrimal gland plasma membranes by aqueous two-phase affinity partitioning

Ekblad, Lars LU ; Jergil, Bengt LU and Gierow, J Peter (2000) In Journal of Chromatography. B 743(1-2). p.397-401
Abstract
We describe the purification of lacrimal gland plasma membranes by affinity partitioning using a two-phase system containing polyethylene glycol and dextran in which wheat germ agglutinin conjugated to dextran is used as affinity ligand. When partitioning a microsomal fraction, the plasma membrane marker 5'-nucleotidase was obtained in the affinity ligand-containing bottom phase, whereas the endoplasmic reticulum marker NADH-ferricyanide reductase remained in the top phase. The affinity partitioning behaviour of components involved in exocytosis and cellular signalling was also examined.
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phosphatidylinositol 4-kinase
in
Journal of Chromatography. B
volume
743
issue
1-2
pages
397 - 401
publisher
Elsevier
external identifiers
  • pmid:10942310
  • scopus:0034705689
ISSN
1387-2273
DOI
10.1016/S0378-4347(00)00152-3
language
English
LU publication?
yes
id
3399b16f-5d44-4011-9650-0ae9859f51d8 (old id 1116765)
date added to LUP
2016-04-01 16:49:47
date last changed
2020-09-09 10:16:35
@article{3399b16f-5d44-4011-9650-0ae9859f51d8,
  abstract     = {We describe the purification of lacrimal gland plasma membranes by affinity partitioning using a two-phase system containing polyethylene glycol and dextran in which wheat germ agglutinin conjugated to dextran is used as affinity ligand. When partitioning a microsomal fraction, the plasma membrane marker 5'-nucleotidase was obtained in the affinity ligand-containing bottom phase, whereas the endoplasmic reticulum marker NADH-ferricyanide reductase remained in the top phase. The affinity partitioning behaviour of components involved in exocytosis and cellular signalling was also examined.},
  author       = {Ekblad, Lars and Jergil, Bengt and Gierow, J Peter},
  issn         = {1387-2273},
  language     = {eng},
  number       = {1-2},
  pages        = {397--401},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography. B},
  title        = {Purification of rabbit lacrimal gland plasma membranes by aqueous two-phase affinity partitioning},
  url          = {http://dx.doi.org/10.1016/S0378-4347(00)00152-3},
  doi          = {10.1016/S0378-4347(00)00152-3},
  volume       = {743},
  year         = {2000},
}