Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C.

Carlsson, Robert LU orcid ; Hjalmarsson, Anna ; Liberg, David LU ; Persson, Christine LU and Leanderson, Tomas LU (2002) In Gene 299(1-2). p.271-278
Abstract
Erythroblast transformation-specific domain (ETS) transcription factors regulate some of the critical molecular mechanisms controlling the differentiation of multipotent haematopoietic progenitor cells into effector B-lymphocytes. The SPI-group ETS-protein transcription factors PU.1 and SPI-B play essential and, although coexpressed and binding to similar DNA sequences, unique roles in B-cell differentiation in mice. Mouse SPI-C is an SPI-group ETS protein expressed temporarily during B-cell development and in macrophages. Here we present the genomic organization of the mouse SPI-C gene, and show by rapid amplification of cDNA ends (5′-RACE) analysis that transcription of the mouse SPI-C mRNA starts at a single site producing a single... (More)
Erythroblast transformation-specific domain (ETS) transcription factors regulate some of the critical molecular mechanisms controlling the differentiation of multipotent haematopoietic progenitor cells into effector B-lymphocytes. The SPI-group ETS-protein transcription factors PU.1 and SPI-B play essential and, although coexpressed and binding to similar DNA sequences, unique roles in B-cell differentiation in mice. Mouse SPI-C is an SPI-group ETS protein expressed temporarily during B-cell development and in macrophages. Here we present the genomic organization of the mouse SPI-C gene, and show by rapid amplification of cDNA ends (5′-RACE) analysis that transcription of the mouse SPI-C mRNA starts at a single site producing a single processed transcript. We have also isolated a cDNA clone encoding the human SPI-C homologue, which displays 65% amino acid identity to the murine protein. In addition, we show that the genomic structure of the human and mouse genes are similar, containing a 5′ non-coding exon followed by five coding exons. Human SPI-C mRNA is preferentially detected in foetal and adult spleen, lymph nodes and at lower levels in bone marrow and foetal liver. Finally a phylogenetic prediction analysis of SPI-group protein sequences suggest that the SPI-C proteins form a distinct subgroup, with human SPI-C being closest related to the mouse SPI-C protein. (Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Evolution, Erythroblast transformation specific domain, PU.1, Transcription, B cell, Exon
in
Gene
volume
299
issue
1-2
pages
271 - 278
publisher
Elsevier
external identifiers
  • pmid:12459275
  • wos:000179841600026
  • scopus:0037121053
ISSN
1879-0038
DOI
10.1016/S0378-1119(02)01078-8
language
English
LU publication?
yes
id
999d53fe-8cf8-4aa7-a047-3b67f96be41a (old id 111677)
date added to LUP
2016-04-01 16:03:43
date last changed
2024-01-11 00:42:55
@article{999d53fe-8cf8-4aa7-a047-3b67f96be41a,
  abstract     = {{Erythroblast transformation-specific domain (ETS) transcription factors regulate some of the critical molecular mechanisms controlling the differentiation of multipotent haematopoietic progenitor cells into effector B-lymphocytes. The SPI-group ETS-protein transcription factors PU.1 and SPI-B play essential and, although coexpressed and binding to similar DNA sequences, unique roles in B-cell differentiation in mice. Mouse SPI-C is an SPI-group ETS protein expressed temporarily during B-cell development and in macrophages. Here we present the genomic organization of the mouse SPI-C gene, and show by rapid amplification of cDNA ends (5′-RACE) analysis that transcription of the mouse SPI-C mRNA starts at a single site producing a single processed transcript. We have also isolated a cDNA clone encoding the human SPI-C homologue, which displays 65% amino acid identity to the murine protein. In addition, we show that the genomic structure of the human and mouse genes are similar, containing a 5′ non-coding exon followed by five coding exons. Human SPI-C mRNA is preferentially detected in foetal and adult spleen, lymph nodes and at lower levels in bone marrow and foetal liver. Finally a phylogenetic prediction analysis of SPI-group protein sequences suggest that the SPI-C proteins form a distinct subgroup, with human SPI-C being closest related to the mouse SPI-C protein.}},
  author       = {{Carlsson, Robert and Hjalmarsson, Anna and Liberg, David and Persson, Christine and Leanderson, Tomas}},
  issn         = {{1879-0038}},
  keywords     = {{Evolution; Erythroblast transformation specific domain; PU.1; Transcription; B cell; Exon}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{271--278}},
  publisher    = {{Elsevier}},
  series       = {{Gene}},
  title        = {{Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C.}},
  url          = {{http://dx.doi.org/10.1016/S0378-1119(02)01078-8}},
  doi          = {{10.1016/S0378-1119(02)01078-8}},
  volume       = {{299}},
  year         = {{2002}},
}