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Interleukin 8 receptor deficiency confers susceptibility to acute experimental pyelonephritis and may have a human counterpart

Frendeus, Björn; Godaly, Gabriela LU ; Hang, Long; Karpman, Diana LU ; Lundstedt, Ann-Charlotte LU and Svanborg, Catharina LU (2000) In Journal of Experimental Medicine 192(6). p.881-890
Abstract
Neutrophils migrate to infected mucosal sites that they protect against invading pathogens. Their interaction with the epithelial barrier is controlled by CXC chemokines and by their receptors. This study examined the change in susceptibility to urinary tract infection (UTI) after deletion of the murine interleukin 8 receptor homologue (mIL-8Rh). Experimental UTIs in control mice stimulated an epithelial chemokine response and increased chemokine receptor expression. Neutrophils migrated through the tissues to the epithelial barrier that they crossed into the lumen, and the mice developed pyuria. In mIL-8Rh knockout (KO) mice, the chemokine response was intact, but the epithelial cells failed to express IL-8R, and neutrophils accumulated... (More)
Neutrophils migrate to infected mucosal sites that they protect against invading pathogens. Their interaction with the epithelial barrier is controlled by CXC chemokines and by their receptors. This study examined the change in susceptibility to urinary tract infection (UTI) after deletion of the murine interleukin 8 receptor homologue (mIL-8Rh). Experimental UTIs in control mice stimulated an epithelial chemokine response and increased chemokine receptor expression. Neutrophils migrated through the tissues to the epithelial barrier that they crossed into the lumen, and the mice developed pyuria. In mIL-8Rh knockout (KO) mice, the chemokine response was intact, but the epithelial cells failed to express IL-8R, and neutrophils accumulated in the tissues. The KO mice were unable to clear bacteria from kidneys and bladders and developed bacteremia and symptoms of systemic disease, but control mice were fully resistant to infection. The experimental UTI model demonstrated that IL-8R-dependent mechanisms control the urinary tract defense, and that neutrophils are essential host effector cells. Patients prone to acute pyelonephritis also showed low CXC chemokine receptor 1 expression compared with age-matched controls, suggesting that chemokine receptor expression may also influence the susceptibility to UTIs in humans. The results provide a first molecular clue to disease susceptibility of patients prone to acute pyelonephritis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chemokine receptor, urinary tract infection, knockout mice, mucosal immunity, lipopolysaccharide
in
Journal of Experimental Medicine
volume
192
issue
6
pages
881 - 890
publisher
Rockefeller University Press
external identifiers
  • pmid:10993918
  • scopus:0033802983
ISSN
1540-9538
language
English
LU publication?
yes
id
b7b9fb81-9584-4e44-aa48-ccbeadc116fa (old id 1117092)
alternative location
http://www.jem.org/cgi/content/full/192/6/881
date added to LUP
2008-07-02 16:24:34
date last changed
2017-06-18 04:24:54
@article{b7b9fb81-9584-4e44-aa48-ccbeadc116fa,
  abstract     = {Neutrophils migrate to infected mucosal sites that they protect against invading pathogens. Their interaction with the epithelial barrier is controlled by CXC chemokines and by their receptors. This study examined the change in susceptibility to urinary tract infection (UTI) after deletion of the murine interleukin 8 receptor homologue (mIL-8Rh). Experimental UTIs in control mice stimulated an epithelial chemokine response and increased chemokine receptor expression. Neutrophils migrated through the tissues to the epithelial barrier that they crossed into the lumen, and the mice developed pyuria. In mIL-8Rh knockout (KO) mice, the chemokine response was intact, but the epithelial cells failed to express IL-8R, and neutrophils accumulated in the tissues. The KO mice were unable to clear bacteria from kidneys and bladders and developed bacteremia and symptoms of systemic disease, but control mice were fully resistant to infection. The experimental UTI model demonstrated that IL-8R-dependent mechanisms control the urinary tract defense, and that neutrophils are essential host effector cells. Patients prone to acute pyelonephritis also showed low CXC chemokine receptor 1 expression compared with age-matched controls, suggesting that chemokine receptor expression may also influence the susceptibility to UTIs in humans. The results provide a first molecular clue to disease susceptibility of patients prone to acute pyelonephritis.},
  author       = {Frendeus, Björn and Godaly, Gabriela and Hang, Long and Karpman, Diana and Lundstedt, Ann-Charlotte and Svanborg, Catharina},
  issn         = {1540-9538},
  keyword      = {chemokine receptor,urinary tract infection,knockout mice,mucosal immunity,lipopolysaccharide},
  language     = {eng},
  number       = {6},
  pages        = {881--890},
  publisher    = {Rockefeller University Press},
  series       = {Journal of Experimental Medicine},
  title        = {Interleukin 8 receptor deficiency confers susceptibility to acute experimental pyelonephritis and may have a human counterpart},
  volume       = {192},
  year         = {2000},
}