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In vivo protection of nigral dopamine neurons by lentiviral gene transfer of the novel GDNF-family member neublastin/artemin

Rosenblad, Carl; Gronborg, M; Hansen, C; Blom, N; Meyer, M; Johansen, J; Dago, L; Kirik, Deniz LU ; Patel, U A and Lundberg, Cecilia LU , et al. (2000) In Molecular and Cellular Neuroscience 15(2). p.199-214
Abstract
The glial cell line-derived neurotrophic factor (GDNF)-family of neurotrophic factors consisted until recently of three members, GDNF, neurturin, and persephin. We describe here the cloning of a new GDNF-family member, neublastin (NBN), identical to artemin (ART), recently published (Baloh et al., 1998). Addition of NBN/ART to cultures of fetal mesencephalic dopamine (DA) neurons increased the number of surviving tyrosine hydroxylase (TH)-immunoreactive neurons by approximately 70%, similar to the maximal effect obtained with GDNF. To investigate the neuroprotective effects in vivo, lentiviral vectors carrying the cDNA for NBN/ART or GDNF were injected into the striatum and ventral midbrain. Three weeks after an intrastriatal... (More)
The glial cell line-derived neurotrophic factor (GDNF)-family of neurotrophic factors consisted until recently of three members, GDNF, neurturin, and persephin. We describe here the cloning of a new GDNF-family member, neublastin (NBN), identical to artemin (ART), recently published (Baloh et al., 1998). Addition of NBN/ART to cultures of fetal mesencephalic dopamine (DA) neurons increased the number of surviving tyrosine hydroxylase (TH)-immunoreactive neurons by approximately 70%, similar to the maximal effect obtained with GDNF. To investigate the neuroprotective effects in vivo, lentiviral vectors carrying the cDNA for NBN/ART or GDNF were injected into the striatum and ventral midbrain. Three weeks after an intrastriatal 6-hydroxydopamine lesion only about 20% of the nigral DA neurons were left in the control group, while 80-90% of the DA neurons remained in the NBN/ART and GDNF treatment groups, and the striatal TH-immunoreactive innervation was partly spared. We conclude that NBN/ART, similarly to GDNF, is a potent neuroprotective factor for the nigrostriatal DA neurons in vivo. (Less)
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publication status
published
subject
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Molecular and Cellular Neuroscience
volume
15
issue
2
pages
199 - 214
publisher
Elsevier
external identifiers
  • pmid:10673327
  • scopus:0033978389
ISSN
1044-7431
DOI
10.1006/mcne.1999.0817
language
English
LU publication?
yes
id
44e66185-9cdf-49de-aff5-4ee7e4b6ff73 (old id 1117182)
date added to LUP
2008-07-03 09:17:01
date last changed
2017-11-19 03:28:31
@article{44e66185-9cdf-49de-aff5-4ee7e4b6ff73,
  abstract     = {The glial cell line-derived neurotrophic factor (GDNF)-family of neurotrophic factors consisted until recently of three members, GDNF, neurturin, and persephin. We describe here the cloning of a new GDNF-family member, neublastin (NBN), identical to artemin (ART), recently published (Baloh et al., 1998). Addition of NBN/ART to cultures of fetal mesencephalic dopamine (DA) neurons increased the number of surviving tyrosine hydroxylase (TH)-immunoreactive neurons by approximately 70%, similar to the maximal effect obtained with GDNF. To investigate the neuroprotective effects in vivo, lentiviral vectors carrying the cDNA for NBN/ART or GDNF were injected into the striatum and ventral midbrain. Three weeks after an intrastriatal 6-hydroxydopamine lesion only about 20% of the nigral DA neurons were left in the control group, while 80-90% of the DA neurons remained in the NBN/ART and GDNF treatment groups, and the striatal TH-immunoreactive innervation was partly spared. We conclude that NBN/ART, similarly to GDNF, is a potent neuroprotective factor for the nigrostriatal DA neurons in vivo.},
  author       = {Rosenblad, Carl and Gronborg, M and Hansen, C and Blom, N and Meyer, M and Johansen, J and Dago, L and Kirik, Deniz and Patel, U A and Lundberg, Cecilia and Trono, D and Bjorklund, A and Johansen, T E},
  issn         = {1044-7431},
  language     = {eng},
  number       = {2},
  pages        = {199--214},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {In vivo protection of nigral dopamine neurons by lentiviral gene transfer of the novel GDNF-family member neublastin/artemin},
  url          = {http://dx.doi.org/10.1006/mcne.1999.0817},
  volume       = {15},
  year         = {2000},
}