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P2Y receptors contribute to ATP-induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells

Arslan, G; Filipeanu, C M; Irenius, E; Kull, B; Clementi, E; Allgaier, C; Erlinge, David LU and Fredholm, B B (2000) In Neuropharmacology 39(3). p.482-496
Abstract
ATP-induced Ca2+ transients were examined in individual PC12 cells of a well defined clone, before and after treatment with nerve growth factor (NGF) to induce a neurone-like phenotype. Using reverse transcriptase PCR these cells were found to express mRNA for several P2 receptors. In undifferentiated cells the ATP-induced Ca2+ response was entirely dependent on Ca2+ influx, could not be mimicked by UTP, alpha,beta-methylene ATP or dibenzoyl ATP or be blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS). ATP had no significant effect on levels of cyclic AMP or inositol 1,4,5-trisphosphate (InsP3). These results suggest that in undifferentiated PC12 cells ATP mainly acts on a P2X receptor, possibly the P2X4 subtype.... (More)
ATP-induced Ca2+ transients were examined in individual PC12 cells of a well defined clone, before and after treatment with nerve growth factor (NGF) to induce a neurone-like phenotype. Using reverse transcriptase PCR these cells were found to express mRNA for several P2 receptors. In undifferentiated cells the ATP-induced Ca2+ response was entirely dependent on Ca2+ influx, could not be mimicked by UTP, alpha,beta-methylene ATP or dibenzoyl ATP or be blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS). ATP had no significant effect on levels of cyclic AMP or inositol 1,4,5-trisphosphate (InsP3). These results suggest that in undifferentiated PC12 cells ATP mainly acts on a P2X receptor, possibly the P2X4 subtype. After treatment with NGF for 7 days the ATP response was increased and partially sensitive to PPADS. A component of the ATP-induced Ca2+ increase was due to mobilisation of intracellular Ca2+ stores and another to capacitative Ca2+ entry. UTP caused an increase in intracellular Ca2+, and InsP3 formation could be stimulated by ATP and UTP. ATP also caused a small increase in cyclic AMP, but this was abolished in the presence of indomethacin. Thus, after NGF treatment ATP acts partially via a P2Y receptor, possibly the P2Y2 subtype. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Nerve Growth Factor, P2X receptors, Purines, Inositol phosphate, Cyclic AMP, UTP
in
Neuropharmacology
volume
39
issue
3
pages
482 - 496
publisher
Elsevier
external identifiers
  • pmid:10698014
  • scopus:0033966635
ISSN
1873-7064
DOI
10.1016/S0028-3908(99)00141-0
language
English
LU publication?
yes
id
252ee673-3dbb-49c6-88cb-c2711345b51a (old id 1117336)
date added to LUP
2008-07-03 11:18:16
date last changed
2017-01-01 04:24:15
@article{252ee673-3dbb-49c6-88cb-c2711345b51a,
  abstract     = {ATP-induced Ca2+ transients were examined in individual PC12 cells of a well defined clone, before and after treatment with nerve growth factor (NGF) to induce a neurone-like phenotype. Using reverse transcriptase PCR these cells were found to express mRNA for several P2 receptors. In undifferentiated cells the ATP-induced Ca2+ response was entirely dependent on Ca2+ influx, could not be mimicked by UTP, alpha,beta-methylene ATP or dibenzoyl ATP or be blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS). ATP had no significant effect on levels of cyclic AMP or inositol 1,4,5-trisphosphate (InsP3). These results suggest that in undifferentiated PC12 cells ATP mainly acts on a P2X receptor, possibly the P2X4 subtype. After treatment with NGF for 7 days the ATP response was increased and partially sensitive to PPADS. A component of the ATP-induced Ca2+ increase was due to mobilisation of intracellular Ca2+ stores and another to capacitative Ca2+ entry. UTP caused an increase in intracellular Ca2+, and InsP3 formation could be stimulated by ATP and UTP. ATP also caused a small increase in cyclic AMP, but this was abolished in the presence of indomethacin. Thus, after NGF treatment ATP acts partially via a P2Y receptor, possibly the P2Y2 subtype.},
  author       = {Arslan, G and Filipeanu, C M and Irenius, E and Kull, B and Clementi, E and Allgaier, C and Erlinge, David and Fredholm, B B},
  issn         = {1873-7064},
  keyword      = {Nerve Growth Factor,P2X receptors,Purines,Inositol phosphate,Cyclic AMP,UTP},
  language     = {eng},
  number       = {3},
  pages        = {482--496},
  publisher    = {Elsevier},
  series       = {Neuropharmacology},
  title        = {P2Y receptors contribute to ATP-induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells},
  url          = {http://dx.doi.org/10.1016/S0028-3908(99)00141-0},
  volume       = {39},
  year         = {2000},
}