P2Y receptors contribute to ATP-induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells
Arslan, G ; Filipeanu, C M ; Irenius, E ; Kull, B ; Clementi, E ; Allgaier, C ; Erlinge, David LU
and Fredholm, B B
(2000)
In Neuropharmacology
39(3).
p.482-496
- Abstract
- ATP-induced Ca2+ transients were examined in individual PC12 cells of a well defined clone, before and after treatment with nerve growth factor (NGF) to induce a neurone-like phenotype. Using reverse transcriptase PCR these cells were found to express mRNA for several P2 receptors. In undifferentiated cells the ATP-induced Ca2+ response was entirely dependent on Ca2+ influx, could not be mimicked by UTP, alpha,beta-methylene ATP or dibenzoyl ATP or be blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS). ATP had no significant effect on levels of cyclic AMP or inositol 1,4,5-trisphosphate (InsP3). These results suggest that in undifferentiated PC12 cells ATP mainly acts on a P2X receptor, possibly the P2X4 subtype.... (More)
- ATP-induced Ca2+ transients were examined in individual PC12 cells of a well defined clone, before and after treatment with nerve growth factor (NGF) to induce a neurone-like phenotype. Using reverse transcriptase PCR these cells were found to express mRNA for several P2 receptors. In undifferentiated cells the ATP-induced Ca2+ response was entirely dependent on Ca2+ influx, could not be mimicked by UTP, alpha,beta-methylene ATP or dibenzoyl ATP or be blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS). ATP had no significant effect on levels of cyclic AMP or inositol 1,4,5-trisphosphate (InsP3). These results suggest that in undifferentiated PC12 cells ATP mainly acts on a P2X receptor, possibly the P2X4 subtype. After treatment with NGF for 7 days the ATP response was increased and partially sensitive to PPADS. A component of the ATP-induced Ca2+ increase was due to mobilisation of intracellular Ca2+ stores and another to capacitative Ca2+ entry. UTP caused an increase in intracellular Ca2+, and InsP3 formation could be stimulated by ATP and UTP. ATP also caused a small increase in cyclic AMP, but this was abolished in the presence of indomethacin. Thus, after NGF treatment ATP acts partially via a P2Y receptor, possibly the P2Y2 subtype. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1117336
- author
- Arslan, G
; Filipeanu, C M
; Irenius, E
; Kull, B
; Clementi, E
; Allgaier, C
; Erlinge, David
LU
and Fredholm, B B
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Nerve Growth Factor, P2X receptors, Purines, Inositol phosphate, Cyclic AMP, UTP
- in
- Neuropharmacology
- volume
- 39
- issue
- 3
- pages
- 482 - 496
- publisher
- Elsevier
- external identifiers
-
- pmid:10698014
- scopus:0033966635
- ISSN
- 1873-7064
- DOI
- 10.1016/S0028-3908(99)00141-0
- language
- English
- LU publication?
- yes
- id
- 252ee673-3dbb-49c6-88cb-c2711345b51a (old id 1117336)
- date added to LUP
- 2016-04-01 11:38:50
- date last changed
- 2022-01-26 08:05:37
@article{252ee673-3dbb-49c6-88cb-c2711345b51a,
abstract = {{ATP-induced Ca2+ transients were examined in individual PC12 cells of a well defined clone, before and after treatment with nerve growth factor (NGF) to induce a neurone-like phenotype. Using reverse transcriptase PCR these cells were found to express mRNA for several P2 receptors. In undifferentiated cells the ATP-induced Ca2+ response was entirely dependent on Ca2+ influx, could not be mimicked by UTP, alpha,beta-methylene ATP or dibenzoyl ATP or be blocked by pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS). ATP had no significant effect on levels of cyclic AMP or inositol 1,4,5-trisphosphate (InsP3). These results suggest that in undifferentiated PC12 cells ATP mainly acts on a P2X receptor, possibly the P2X4 subtype. After treatment with NGF for 7 days the ATP response was increased and partially sensitive to PPADS. A component of the ATP-induced Ca2+ increase was due to mobilisation of intracellular Ca2+ stores and another to capacitative Ca2+ entry. UTP caused an increase in intracellular Ca2+, and InsP3 formation could be stimulated by ATP and UTP. ATP also caused a small increase in cyclic AMP, but this was abolished in the presence of indomethacin. Thus, after NGF treatment ATP acts partially via a P2Y receptor, possibly the P2Y2 subtype.}},
author = {{Arslan, G and Filipeanu, C M and Irenius, E and Kull, B and Clementi, E and Allgaier, C and Erlinge, David and Fredholm, B B}},
issn = {{1873-7064}},
keywords = {{Nerve Growth Factor; P2X receptors; Purines; Inositol phosphate; Cyclic AMP; UTP}},
language = {{eng}},
number = {{3}},
pages = {{482--496}},
publisher = {{Elsevier}},
series = {{Neuropharmacology}},
title = {{P2Y receptors contribute to ATP-induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells}},
url = {{http://dx.doi.org/10.1016/S0028-3908(99)00141-0}},
doi = {{10.1016/S0028-3908(99)00141-0}},
volume = {{39}},
year = {{2000}},
}