Effects of transforming growth factor beta1 expression in a rat colon carcinoma : growth inhibition, leukocyte infiltration and production of interleukin-10 and tumor necrosis factor alpha
(2000) In Cancer Immunology and Immunotherapy 48(10). p.579-587- Abstract
The cytokine transforming growth factor beta-1 (TGFbeta1), was transfected into a TGFbeta1-negative rat colon carcinoma. The growth of isografts of TGFbeta1-expressing tumors was compared to that of vector control transfectants. The TGFbeta1 transfectant grew significantly more slowly after intrahepatic isografting than did vector control and wild-type tumors. The TGFbeta1-transfected tumor tissue had significantly greater infiltration of both CD4+ and CD8+ T lymphocytes than did the vector control tumor. The tumor-infiltrating leukocytes (TIL) from TGFbeta1-transfected tumor secreted significantly more of the cytokines interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFalpha) than did TIL from the vector control tumor. The... (More)
The cytokine transforming growth factor beta-1 (TGFbeta1), was transfected into a TGFbeta1-negative rat colon carcinoma. The growth of isografts of TGFbeta1-expressing tumors was compared to that of vector control transfectants. The TGFbeta1 transfectant grew significantly more slowly after intrahepatic isografting than did vector control and wild-type tumors. The TGFbeta1-transfected tumor tissue had significantly greater infiltration of both CD4+ and CD8+ T lymphocytes than did the vector control tumor. The tumor-infiltrating leukocytes (TIL) from TGFbeta1-transfected tumor secreted significantly more of the cytokines interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFalpha) than did TIL from the vector control tumor. The TGFbeta1 transfectant also demonstrated a significantly slower outgrowth in immunodeficient SCID mice, supporting a non-T-lymphocyte-dependent mechanism for the tumor retardation. In SCID mice, the TGFbeta1-transfected tumor demonstrated significantly greater infiltration of both granulocytes and macrophages than did the vector control transfectant. We also demonstrated a direct inhibitory effect of rat TNFalpha on tumor proliferation in vitro. These results suggest that TGFbeta1 induces a local secretion of immunomodulating cytokines and that this may influence monocytes, lymphocytes and granulocytes to retard tumor outgrowth.
(Less)
- author
- Schiött, A LU ; Widegren, B LU ; Sjögren, H O LU ; Lindvall, M LU and C Johansson, Anna LU
- organization
- publishing date
- 2000-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Carcinoma, Cell Movement, Chimera, Colonic Neoplasms, Cytokines, Growth Inhibitors, Interleukin-10, Lymphocytes, Tumor-Infiltrating, Mice, Mice, SCID, Rats, Rats, Inbred F344, Rats, Inbred WF, Recombinant Proteins, Transforming Growth Factor beta, Tumor Necrosis Factor-alpha, Journal Article, Research Support, Non-U.S. Gov't
- in
- Cancer Immunology and Immunotherapy
- volume
- 48
- issue
- 10
- pages
- 9 pages
- publisher
- Springer
- external identifiers
-
- pmid:10630310
- pmid:10630310
- scopus:0033988217
- ISSN
- 1432-0851
- DOI
- 10.1007/PL00006676
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100), Neurosurgery (013026000), Faculty of Medicine (000022000), Division IV (013230800)
- id
- dd2ec217-33b4-4e48-aa2e-a6fb0847f6e8 (old id 1117668)
- date added to LUP
- 2016-04-01 15:35:02
- date last changed
- 2022-01-28 06:05:35
@article{dd2ec217-33b4-4e48-aa2e-a6fb0847f6e8,
abstract = {{<p>The cytokine transforming growth factor beta-1 (TGFbeta1), was transfected into a TGFbeta1-negative rat colon carcinoma. The growth of isografts of TGFbeta1-expressing tumors was compared to that of vector control transfectants. The TGFbeta1 transfectant grew significantly more slowly after intrahepatic isografting than did vector control and wild-type tumors. The TGFbeta1-transfected tumor tissue had significantly greater infiltration of both CD4+ and CD8+ T lymphocytes than did the vector control tumor. The tumor-infiltrating leukocytes (TIL) from TGFbeta1-transfected tumor secreted significantly more of the cytokines interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFalpha) than did TIL from the vector control tumor. The TGFbeta1 transfectant also demonstrated a significantly slower outgrowth in immunodeficient SCID mice, supporting a non-T-lymphocyte-dependent mechanism for the tumor retardation. In SCID mice, the TGFbeta1-transfected tumor demonstrated significantly greater infiltration of both granulocytes and macrophages than did the vector control transfectant. We also demonstrated a direct inhibitory effect of rat TNFalpha on tumor proliferation in vitro. These results suggest that TGFbeta1 induces a local secretion of immunomodulating cytokines and that this may influence monocytes, lymphocytes and granulocytes to retard tumor outgrowth.</p>}},
author = {{Schiött, A and Widegren, B and Sjögren, H O and Lindvall, M and C Johansson, Anna}},
issn = {{1432-0851}},
keywords = {{Animals; Carcinoma; Cell Movement; Chimera; Colonic Neoplasms; Cytokines; Growth Inhibitors; Interleukin-10; Lymphocytes, Tumor-Infiltrating; Mice; Mice, SCID; Rats; Rats, Inbred F344; Rats, Inbred WF; Recombinant Proteins; Transforming Growth Factor beta; Tumor Necrosis Factor-alpha; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
number = {{10}},
pages = {{579--587}},
publisher = {{Springer}},
series = {{Cancer Immunology and Immunotherapy}},
title = {{Effects of transforming growth factor beta1 expression in a rat colon carcinoma : growth inhibition, leukocyte infiltration and production of interleukin-10 and tumor necrosis factor alpha}},
url = {{http://dx.doi.org/10.1007/PL00006676}},
doi = {{10.1007/PL00006676}},
volume = {{48}},
year = {{2000}},
}