Advanced

Effects of transforming growth factor beta1 expression in a rat colon carcinoma : growth inhibition, leukocyte infiltration and production of interleukin-10 and tumor necrosis factor alpha

Schiött, A LU ; Widegren, B LU ; Sjögren, H O LU ; Lindvall, M LU and C Johansson, Anna LU (2000) In Cancer Immunology and Immunotherapy 48(10). p.579-587
Abstract

The cytokine transforming growth factor beta-1 (TGFbeta1), was transfected into a TGFbeta1-negative rat colon carcinoma. The growth of isografts of TGFbeta1-expressing tumors was compared to that of vector control transfectants. The TGFbeta1 transfectant grew significantly more slowly after intrahepatic isografting than did vector control and wild-type tumors. The TGFbeta1-transfected tumor tissue had significantly greater infiltration of both CD4+ and CD8+ T lymphocytes than did the vector control tumor. The tumor-infiltrating leukocytes (TIL) from TGFbeta1-transfected tumor secreted significantly more of the cytokines interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFalpha) than did TIL from the vector control tumor. The... (More)

The cytokine transforming growth factor beta-1 (TGFbeta1), was transfected into a TGFbeta1-negative rat colon carcinoma. The growth of isografts of TGFbeta1-expressing tumors was compared to that of vector control transfectants. The TGFbeta1 transfectant grew significantly more slowly after intrahepatic isografting than did vector control and wild-type tumors. The TGFbeta1-transfected tumor tissue had significantly greater infiltration of both CD4+ and CD8+ T lymphocytes than did the vector control tumor. The tumor-infiltrating leukocytes (TIL) from TGFbeta1-transfected tumor secreted significantly more of the cytokines interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFalpha) than did TIL from the vector control tumor. The TGFbeta1 transfectant also demonstrated a significantly slower outgrowth in immunodeficient SCID mice, supporting a non-T-lymphocyte-dependent mechanism for the tumor retardation. In SCID mice, the TGFbeta1-transfected tumor demonstrated significantly greater infiltration of both granulocytes and macrophages than did the vector control transfectant. We also demonstrated a direct inhibitory effect of rat TNFalpha on tumor proliferation in vitro. These results suggest that TGFbeta1 induces a local secretion of immunomodulating cytokines and that this may influence monocytes, lymphocytes and granulocytes to retard tumor outgrowth.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Carcinoma, Cell Movement, Chimera, Colonic Neoplasms, Cytokines, Growth Inhibitors, Interleukin-10, Lymphocytes, Tumor-Infiltrating, Mice, Mice, SCID, Rats, Rats, Inbred F344, Rats, Inbred WF, Recombinant Proteins, Transforming Growth Factor beta, Tumor Necrosis Factor-alpha, Journal Article, Research Support, Non-U.S. Gov't
in
Cancer Immunology and Immunotherapy
volume
48
issue
10
pages
9 pages
publisher
Springer
external identifiers
  • pmid:10630310
  • scopus:0033988217
ISSN
1432-0851
DOI
10.1007/PL00006676
language
English
LU publication?
yes
id
dd2ec217-33b4-4e48-aa2e-a6fb0847f6e8 (old id 1117668)
date added to LUP
2008-06-27 11:16:35
date last changed
2017-01-03 07:45:16
@article{dd2ec217-33b4-4e48-aa2e-a6fb0847f6e8,
  abstract     = {<p>The cytokine transforming growth factor beta-1 (TGFbeta1), was transfected into a TGFbeta1-negative rat colon carcinoma. The growth of isografts of TGFbeta1-expressing tumors was compared to that of vector control transfectants. The TGFbeta1 transfectant grew significantly more slowly after intrahepatic isografting than did vector control and wild-type tumors. The TGFbeta1-transfected tumor tissue had significantly greater infiltration of both CD4+ and CD8+ T lymphocytes than did the vector control tumor. The tumor-infiltrating leukocytes (TIL) from TGFbeta1-transfected tumor secreted significantly more of the cytokines interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFalpha) than did TIL from the vector control tumor. The TGFbeta1 transfectant also demonstrated a significantly slower outgrowth in immunodeficient SCID mice, supporting a non-T-lymphocyte-dependent mechanism for the tumor retardation. In SCID mice, the TGFbeta1-transfected tumor demonstrated significantly greater infiltration of both granulocytes and macrophages than did the vector control transfectant. We also demonstrated a direct inhibitory effect of rat TNFalpha on tumor proliferation in vitro. These results suggest that TGFbeta1 induces a local secretion of immunomodulating cytokines and that this may influence monocytes, lymphocytes and granulocytes to retard tumor outgrowth.</p>},
  author       = {Schiött, A and Widegren, B and Sjögren, H O and Lindvall, M and C Johansson, Anna},
  issn         = {1432-0851},
  keyword      = {Animals,Carcinoma,Cell Movement,Chimera,Colonic Neoplasms,Cytokines,Growth Inhibitors,Interleukin-10,Lymphocytes, Tumor-Infiltrating,Mice,Mice, SCID,Rats,Rats, Inbred F344,Rats, Inbred WF,Recombinant Proteins,Transforming Growth Factor beta,Tumor Necrosis Factor-alpha,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {10},
  pages        = {579--587},
  publisher    = {Springer},
  series       = {Cancer Immunology and Immunotherapy},
  title        = {Effects of transforming growth factor beta1 expression in a rat colon carcinoma : growth inhibition, leukocyte infiltration and production of interleukin-10 and tumor necrosis factor alpha},
  url          = {http://dx.doi.org/10.1007/PL00006676},
  volume       = {48},
  year         = {2000},
}